{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,5]],"date-time":"2026-01-05T06:39:38Z","timestamp":1767595178217,"version":"3.48.0"},"reference-count":51,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,1,5]],"date-time":"2026-01-05T00:00:00Z","timestamp":1767571200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Cell. Infect. Microbiol."],"abstract":"<jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Alveolar echinococcosis (AE), a severe parasitic infection often likened to \"parasitic cancer\", still lacks effective treatments. Although our earlier ork on the P29 monoclonal antibody (P29 mAb) against the parasite-derived P29 antigen showed potential, its efficacy remained limited, prompting the need for improved biologic agents.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      We applied \u03b2-hydroxybutyrylation (Kbhb) modification to engineer an enhanced antibody, P29 mAb\n                      <jats:sup>Kbhb<\/jats:sup>\n                      , and comprehensively evaluated its properties using surface plasmon resonance, protease resistance assays, pharmacokinetic studies in C57BL\/6 mice, and histopathological analysis of alveolar hydatid cysts.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      The modified antibody retained high antigen-binding affinity (KD = 343 pM) and exhibited markedly increased resistance to proteolytic degradation, with a 1.75-fold improvement in serum persistence after 5 weeks. Furthermore, in a murine model of AE, P29 mAb\n                      <jats:sup>Kbhb<\/jats:sup>\n                      significantly inhibited protoscolex regeneration and induced apoptosis of cyst wall cells relative to the unmodified antibody.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Our results establish a novel connection between protein engineering and antiparasitic therapy, illustrating that Kbhb modification not only augments the efficacy of anti-AE antibodies but also offers a versatile strategy for enhancing antibody stability and half-life. This offers a potential strategy for developing new treatments against neglected zoonotic diseases via tailored post-translational modifications.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.3389\/fcimb.2025.1716047","type":"journal-article","created":{"date-parts":[[2026,1,5]],"date-time":"2026-01-05T06:36:53Z","timestamp":1767595013000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["Enhancing the structural stability of P29-targeted monoclonal antibodies via \u03b2-hydroxybutyrylation modification improves their therapeutic performance in alveolar echinococcosis"],"prefix":"10.3389","volume":"15","author":[{"given":"Shiqin","family":"Yuan","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tao","family":"Li","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lei","family":"Niu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yazhou","family":"Zhu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lang","family":"Wu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wei","family":"Zhao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ming","family":"Li","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zihua","family":"Li","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1965","published-online":{"date-parts":[[2026,1,5]]},"reference":[{"key":"B36","doi-asserted-by":"publisher","first-page":"874","DOI":"10.1038\/s41419-024-07268-3","article-title":"\u03b2-Hydroxybutyrate suppresses M1 macrophage polarization through \u03b2-hydroxybutyrylation of the STAT1 protein","volume":"15","author":"Bai","year":"2024","journal-title":"Cell. 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