{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,7]],"date-time":"2026-01-07T06:57:37Z","timestamp":1767769057768,"version":"3.48.0"},"reference-count":22,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,1,7]],"date-time":"2026-01-07T00:00:00Z","timestamp":1767744000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100018556","name":"Science and Technology Program of Suzhou","doi-asserted-by":"publisher","award":["SKY2022062, SYW2024013, SKYD2022150, SKYD2023158, SKYD2023225, SYW2025058, SYW2025151, SYW2024014"],"award-info":[{"award-number":["SKY2022062, SYW2024013, SKYD2022150, SKYD2023158, SKYD2023225, SYW2025058, SYW2025151, SYW2024014"]}],"id":[{"id":"10.13039\/501100018556","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100022944","name":"Suzhou Gusu Medical Youth Talent","doi-asserted-by":"publisher","award":["GSWS2023063, GSWS2022086, GSWS2022090"],"award-info":[{"award-number":["GSWS2023063, GSWS2022086, GSWS2022090"]}],"id":[{"id":"10.13039\/100022944","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004608","name":"Natural Science Foundation of Jiangsu Province","doi-asserted-by":"publisher","award":["Ym2023074, FLKT2024002, K2024075"],"award-info":[{"award-number":["Ym2023074, FLKT2024002, K2024075"]}],"id":[{"id":"10.13039\/501100004608","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Cell. Infect. Microbiol."],"abstract":"<jats:sec>\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>To evaluate EBUS-TBNA needle rinse fluid versus biopsy tissue for molecular diagnosis of intrathoracic lymph node tuberculosis (TB).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Retrospective analysis of 63 patients with intrathoracic lymph node TB undergoing EBUS-TBNA (2018\u20132024). Rinse fluid and biopsy tissue were tested via TB-DNA (n=32) and Xpert MTB\/RIF (n=31); positivity rates compared.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>The study cohort had a median age of 31 years (interquartile range: 25\u201350.1 years), with 57.1% (36\/63) being male. The most frequently sampled lymph node stations were subcarinal (station 7, 82.5%) and right lower mediastinal (station 4R, 66.7%). Clinically, 82.5% (52\/63) of patients had concomitant pulmonary TB, while 17.5% (11\/63) presented with isolated intrathoracic lymph node TB. For TB-DNA detection, the positivity rate of rinse fluid (71.9%, 23\/32) was significantly higher than that of biopsy tissue (46.9%, 15\/32; \u03c7\u00b2=4.146, P = 0.042). Similarly, the Xpert MTB\/RIF assay showed a higher positivity rate in rinse fluid (77.4%, 24\/31) compared to tissue (41.9%, 13\/31; \u03c7\u00b2=8.11, P = 0.004).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>EBUS-TBNA rinse fluid demonstrates higher sensitivity than biopsy tissue for intrathoracic lymph node TB via TB-DNA\/Xpert MTB\/RIF. Routine rinse fluid testing improves diagnostic yield.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.3389\/fcimb.2025.1734956","type":"journal-article","created":{"date-parts":[[2026,1,7]],"date-time":"2026-01-07T06:52:45Z","timestamp":1767768765000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["EBUS-TBNA needle rinse fluid: a superior specimen for the molecular diagnosis of intrathoracic lymph node tuberculosis"],"prefix":"10.3389","volume":"15","author":[{"given":"Yayan","family":"Niu","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaohong","family":"Li","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jin yu","family":"Yan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Huafeng","family":"Song","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lin","family":"Yao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lijuan","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Meiying","family":"Wu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peijun","family":"Tang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Junchi","family":"Xu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yanjun","family":"Feng","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1965","published-online":{"date-parts":[[2026,1,7]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"797","DOI":"10.5588\/ijtld.18.0757","article-title":"Changes in tuberculosis epidemiology, United States 1993-2017","volume":"23","author":"Armstrong","year":"2019","journal-title":"Int. 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