{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,26]],"date-time":"2026-10-26T19:02:02Z","timestamp":1793041322230,"version":"4.5.0"},"reference-count":42,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,1,14]],"date-time":"2026-01-14T00:00:00Z","timestamp":1768348800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Cell. Infect. Microbiol."],"abstract":"<jats:sec>\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>\n                      To elucidate the molecular epidemiology, virulence repertoire and resistance gene characteristics of carbapenem-resistant\n                      <jats:italic>Enterobacter cloacae<\/jats:italic>\n                      complex (CRECC) in bloodstream infections (BSI), thereby providing evidence for precision therapy and infection control.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>We retrospectively collected 13 non-replicate CRECC-BSI isolates from January 2019 to December 2023 at a tertiary-care hospital in Shandong Province, China. Antimicrobial susceptibility was determined by broth microdilution; Illumina NovaSeq whole-genome sequencing was performed, and genomes were assembled with ABySS and GapCloser. ResFinder, VFDB, CGE and NCBI Pathogen Detection databases were used jointly to analyze resistance genes, virulence factors, plasmid replicons, MLST an SNP-based phylogenetic tree assessed inter-strain relatedness; while filter-mating assays determined the transferability of plasmids.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      A total of 13 CRECC isolates yielded five sequence types (STs), with ST171 predominating (46.2%, 6\/13); all carried\n                      <jats:italic>bla<\/jats:italic>\n                      <jats:sub>NDM<\/jats:sub>\n                      (\n                      <jats:italic>bla<\/jats:italic>\n                      <jats:sub>NDM-1<\/jats:sub>\n                      in 9 isolates,\n                      <jats:italic>bla<\/jats:italic>\n                      <jats:sub>NDM-5<\/jats:sub>\n                      in 4), along with AmpC, ESBLs, and aminoglycoside\/quinolone resistance genes. The IncX3 plasmid replicon was most frequent (46.2%, 6\/13), followed by IncHI2\/HI2A (38.5%, 5\/13). Each strain harbored adherence, biofilm formation, iron\/manganese transport and T6SS virulence genes. Antimicrobial susceptibility testing revealed complete resistance among all isolates to cephalosporins, carbapenems and \u03b2-lactam\/\u03b2-lactamase-inhibitor combinations, while amikacin, tigecycline and polymyxin B remained 100% susceptible. cgMLST revealed a polyclonal population structure. Conjugation assays demonstrated transfer of\n                      <jats:italic>bla<\/jats:italic>\n                      <jats:sub>NDM<\/jats:sub>\n                      -bearing plasmids to recipient\n                      <jats:italic>Escherichia coli<\/jats:italic>\n                      J53.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>\n                      Our institutional CRECC-BSI is characterized by diverse sequence types, a complex plasmid profile and a high burden of virulence genes; ST171 is the dominant clone and\n                      <jats:italic>bla<\/jats:italic>\n                      <jats:sub>NDM-1<\/jats:sub>\n                      the principal carbapenemase. Close surveillance of this high-risk lineage and of IncX3\/IncHI2-mediated horizontal gene transfer is essential, together with strengthened infection-control and antimicrobial-stewardship measures.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.3389\/fcimb.2025.1738317","type":"journal-article","created":{"date-parts":[[2026,1,14]],"date-time":"2026-01-14T06:30:48Z","timestamp":1768372248000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":4,"title":["Bloodstream infection with NDM-1\/5 Enterobacter cloacae complex in China: diverse STs, multi-virulence systems and carbapenem resistance"],"prefix":"10.3389","volume":"15","author":[{"given":"Xinying","family":"Wang","sequence":"first","affiliation":[{"name":"Department of Laboratory Medicine, The Affiliated Taian City Central Hospital of Qingdao University","place":["Taian, China"]},{"name":"Shandong Provincial Key Medical and Health Laboratory of Anti-Drug Resistant Drug Research, The Affiliated Taian City Central Hospital of Qingdao University","place":["Taian, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yujing","family":"Tian","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, The Affiliated Taian City Central Hospital of Qingdao University","place":["Taian, China"]},{"name":"Shandong Provincial Key Medical and Health Laboratory of Anti-Drug Resistant Drug Research, The Affiliated Taian City Central Hospital of Qingdao University","place":["Taian, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qun","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Blood Transfusion, The Affiliated Taian City Central Hospital of Qingdao University","place":["Taian, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan","family":"Jin","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, Shandong Provincial Hospital Affiliated to Shandong First Medical University; Shandong Provincial Hospital","place":["Jinan, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chunhong","family":"Shao","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, Shandong Provincial Hospital Affiliated to Shandong First Medical University; Shandong Provincial Hospital","place":["Jinan, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhijun","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, The Affiliated Taian City Central Hospital of Qingdao University","place":["Taian, China"]},{"name":"Shandong Provincial Key Medical and Health Laboratory of Anti-Drug Resistant Drug Research, The Affiliated Taian City Central Hospital of Qingdao University","place":["Taian, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2026,1,14]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2019.00044","article-title":"Multidrug-resistant Enterobacter cloacae complex emerging as a global, diversifying threat","volume":"10","author":"Annavajhala","year":"2019","journal-title":"Front. 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