{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T03:12:36Z","timestamp":1777864356748,"version":"3.51.4"},"reference-count":44,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,4,30]],"date-time":"2026-04-30T00:00:00Z","timestamp":1777507200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Cell. Infect. Microbiol."],"abstract":"<jats:sec>\n                    <jats:title>Introduction<\/jats:title>\n                    <jats:p>\n                      <jats:italic>Myroides odoratimimus<\/jats:italic>\n                      is a Gram-negative opportunistic pathogen primarily affecting immunocompromised individuals. However, its resistance characteristics and therapeutic response in cancer patients remain poorly defined. This study investigated the antimicrobial resistance profile of a urinary\n                      <jats:italic>M. odoratimimus<\/jats:italic>\n                      isolate from a gastric cancer patient and evaluated the influence of environmental pH on antibiotic efficacy.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      The isolate was identified using the VITEK 2-Compact system, matrix-assisted laser desorption\/ionization time-of-flight mass spectrometry (MALDI-TOF MS), and 16S ribosomal ribonucleic acid (16S rRNA) sequencing. Antimicrobial susceptibility testing against 24 antibiotics was performed using the VITEK 2 system according to Clinical and Laboratory Standards Institute (CLSI) M100 (Ed33) guidelines. Resistance genes were detected by polymerase chain reaction (PCR) and sequencing. The impact of pH (5.5\u20138.5) on antibiotic activity was assessed using disk diffusion.\n                      <jats:italic>In vivo<\/jats:italic>\n                      efficacy of minocycline was evaluated in a murine urinary tract infection model with controlled urine pH modulation.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      The isolate showed 100% sequence identity with\n                      <jats:italic>M. odoratimimus<\/jats:italic>\n                      PR63069 and exhibited multidrug resistance, remaining susceptible only to minocycline. Molecular analysis confirmed the presence of the \u03b2-lactamase gene (blaMUS-1). Tigecycline and minocycline demonstrated optimal activity under neutral conditions (pH 7.3), whereas piperacillin\u2013tazobactam showed relatively improved activity at alkaline pH.\n                      <jats:italic>In vivo<\/jats:italic>\n                      , urinary alkalization (pH 8.5) significantly enhanced the antibacterial efficacy of minocycline.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>\n                      <jats:italic>M. odoratimimus<\/jats:italic>\n                      isolated from a gastric cancer patient demonstrated multidrug resistance mediated by blaMUS-1. Antibiotic activity varied with pH, and urine alkalinization improved minocycline efficacy, suggesting potential strategies for clinical management of Myroides infections.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.3389\/fcimb.2026.1647777","type":"journal-article","created":{"date-parts":[[2026,4,30]],"date-time":"2026-04-30T05:40:31Z","timestamp":1777527631000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["Unusual case of Myroides odoratimimus infection in a cancer patient: addressing its antimicrobial resistance mechanisms and review of the literature"],"prefix":"10.3389","volume":"16","author":[{"given":"Lingying","family":"Fan","sequence":"first","affiliation":[{"name":"Department of Clinical Lab, West China School of Public Health and West China Fourth Hospital, Sichuan University","place":["Chengdu, China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yiping","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Clinical Lab, West China School of Public Health and West China Fourth Hospital, Sichuan University","place":["Chengdu, China"]}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1965","published-online":{"date-parts":[[2026,4,30]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"1751","DOI":"10.2174\/1568026622666220726092034","article-title":"Omics technologies for natural compounds-based drug development","volume":"22","author":"Aborode","year":"2022","journal-title":"CTMC"},{"key":"B2","doi-asserted-by":"publisher","first-page":"67","DOI":"10.1016\/j.nmni.2015.06.007","article-title":"Mus-2, a novel variant of the chromosome-encoded \u03b2-lactamase MUS-1, from Myroides odoratimimus","volume":"7","author":"Al-Bayssari","year":"2015","journal-title":"New Microbes New Infections"},{"key":"B3","doi-asserted-by":"publisher","DOI":"10.1016\/j.virusres.2023.199049","article-title":"Characterization, genome analysis and antibiofilm efficacy of lytic Proteus phages RP6 and RP7 isolated from university hospital sewage","volume":"326","author":"Ali","year":"2023","journal-title":"Virus Res."},{"key":"B4","doi-asserted-by":"publisher","DOI":"10.4081\/ejtm.2022.10813","article-title":"Antibiotic use in endodontic treatment during pregnancy: a narrative review","volume":"32","author":"Aliabadi","year":"2022","journal-title":"Eur. J. Transl. Myol"},{"key":"B5","doi-asserted-by":"publisher","first-page":"100115","DOI":"10.1016\/j.simyco.2021.100115","article-title":"Aspergillus fumigatus and aspergillosis: from basics to clinics","volume":"100","author":"Arastehfar","year":"2021","journal-title":"Stud. Mycol."},{"key":"B6","doi-asserted-by":"publisher","DOI":"10.1128\/aac.00216-22","article-title":"Oxa-48-like \u03b2-lactamases: global epidemiology, treatment options, and development pipeline","volume":"66","author":"Boyd","year":"2022","journal-title":"Antimicrob. Agents Chemother."},{"key":"B7","doi-asserted-by":"publisher","first-page":"696","DOI":"10.1128\/aac.00652-07","article-title":"Prevalence of sulfonamide resistance genes in bacterial isolates from manured agricultural soils and pig slurry in the United Kingdom","volume":"53","author":"Byrne-Bailey","year":"2009","journal-title":"Antimicrob. Agents Chemother."},{"key":"B8","doi-asserted-by":"publisher","first-page":"2070","DOI":"10.1093\/jac\/dkq269","article-title":"Complete nucleotide sequence of the IncN plasmid pKOX105 encoding VIM-1, QnrS1 and SHV-12 proteins in Enterobacteriaceae from Bolzano, Italy compared with IncN plasmids encoding KPC enzymes in the USA","volume":"65","author":"Carattoli","year":"2010","journal-title":"J. Antimicrob. Chemother."},{"key":"B9","doi-asserted-by":"publisher","first-page":"944","DOI":"10.1001\/jamainternmed.2023.2986","article-title":"Efficacy of a clinical decision rule to enable direct oral challenge in patients with low-risk penicillin allergy","volume":"183","author":"Copaescu","year":"2023","journal-title":"JAMA Intern. Med."},{"key":"B10","doi-asserted-by":"publisher","DOI":"10.3390\/antibiotics12081262","article-title":"Antibiotic therapy strategies for treating gram-negative severe infections in the critically ill: a narrative review","volume":"12","author":"Corona","year":"2023","journal-title":"Antibiotics"},{"key":"B11","doi-asserted-by":"publisher","first-page":"3463","DOI":"10.1128\/aac.00543-08","article-title":"Plasmid-encoded carbapenem-hydrolyzing \u03b2-lactamase OXA-48 in an imipenem-susceptible Klebsiella pneumoniae strain from Belgium","volume":"52","author":"Cuzon","year":"2008","journal-title":"Antimicrob. Agents Chemother."},{"key":"B12","doi-asserted-by":"publisher","first-page":"490","DOI":"10.1093\/jac\/dkp498","article-title":"Development of a set of multiplex PCR assays for the detection of genes encoding important \u03b2-lactamases in Enterobacteriaceae","volume":"65","author":"Dallenne","year":"2010","journal-title":"J. Antimicrob. Chemother."},{"key":"B13","doi-asserted-by":"publisher","first-page":"1848","DOI":"10.1016\/j.jiph.2021.11.002","article-title":"Drug resistance in Candida albicans isolates and related changes in the structural domain of Mdr1 protein","volume":"14","author":"Dhasarathan","year":"2021","journal-title":"J. Infection Public Health"},{"key":"B14","doi-asserted-by":"publisher","first-page":"F615","DOI":"10.1152\/ajprenal.00174.2024","article-title":"Sex differences in renal acid-base regulation","volume":"329","author":"Dominguez Rieg","year":"2025","journal-title":"Am. J. Physiol. Renal Physiol."},{"key":"B15","doi-asserted-by":"publisher","first-page":"482","DOI":"10.1097\/inf.0000000000003489","article-title":"Relationship between COVID-19 lockdown and epidemiology of neonatal sepsis","volume":"41","author":"Dutta","year":"2022","journal-title":"Pediatr. Infect. Dis. J."},{"key":"B16","doi-asserted-by":"publisher","first-page":"125","DOI":"10.1016\/s0378-1097(03)00505-6","article-title":"In vitro conjugal transfer of tetracycline resistance from Lactobacillus isolates to other Gram-positive bacteria","volume":"225","author":"Gevers","year":"2003","journal-title":"FEMS Microbiol. Lett."},{"key":"B17","doi-asserted-by":"publisher","first-page":"1703","DOI":"10.1007\/s40265-021-01611-0","article-title":"The antifungal pipeline: fosmanogepix, ibrexafungerp, olorofim, opelconazole, and rezafungin","volume":"81","author":"Hoenigl","year":"2021","journal-title":"Drugs"},{"key":"B18","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.01805-22","article-title":"Novel clinical mNGS-based machine learning model for rapid antimicrobial susceptibility testing of Acinetobacter baumannii","volume":"61","author":"Hu","year":"2023","journal-title":"J. Clin. Microbiol."},{"key":"B19","doi-asserted-by":"publisher","first-page":"497","DOI":"10.1016\/j.xphs.2024.10.028","article-title":"Impact of dissolution medium pH and ionization state of the drug on the release performance of amorphous solid dispersions","volume":"114","author":"Indulkar","year":"2025","journal-title":"J. Pharm. Sci."},{"key":"B20","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2022.823684","article-title":"Global threat of carbapenem-resistant gram-negative bacteria","volume":"12","author":"Jean","year":"2022","journal-title":"Front. Cell. Infect. Microbiol."},{"key":"B21","doi-asserted-by":"publisher","first-page":"781","DOI":"10.1016\/j.chom.2023.04.010","article-title":"Fusobacterium nucleatum-derived succinic acid induces tumor resistance to immunotherapy in colorectal cancer","volume":"31","author":"Jiang","year":"2023","journal-title":"Cell. Host Microbe"},{"key":"B22","doi-asserted-by":"publisher","first-page":"16678","DOI":"10.1038\/s41598-023-42928-y","article-title":"Down regulation of cathepsin W is associated with poor prognosis in pancreatic cancer","volume":"13","author":"Khojasteh-Leylakoohi","year":"2023","journal-title":"Sci. Rep."},{"key":"B23","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2305465120","article-title":"Antimicrobial resistance heterogeneity among multidrug-resistant gram-negative pathogens: phenotypic, genotypic, and proteomic analysis","volume":"120","author":"Mehrotra","year":"2023","journal-title":"Proc. Natl. Acad. Sci. U.S.A."},{"key":"B24","first-page":"6","article-title":"The effect of the level of lipid peroxidation and the pH value of a liposomal suspension on its antibacterial activity","author":"Mel\u2019iantseva","year":"1995","journal-title":"Zh. Mikrobiol. Epidemiol. Immunobiol"},{"key":"B25","doi-asserted-by":"publisher","first-page":"798","DOI":"10.1128\/aac.01185-07","article-title":"Carbapenem-resistant isolates of Klebsiella pneumoniae in China and detection of a conjugative plasmid (bla KPC-2 plus qnrB4) and ablaIMP-4 gene","volume":"52","author":"Mendes","year":"2008","journal-title":"Antimicrob. Agents Chemother."},{"key":"B26","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0024552","article-title":"Plasmidic qnrA3 enhances Escherichia coli fitness in absence of antibiotic exposure","volume":"6","author":"Michon","year":"2011","journal-title":"PloS One"},{"key":"B27","doi-asserted-by":"publisher","first-page":"1029","DOI":"10.2174\/1389450116666151001105622","article-title":"B1-metallo-\u03b2-lactamases: where do we stand","volume":"17","author":"Mojica","year":"2016","journal-title":"CDT"},{"key":"B28","doi-asserted-by":"publisher","DOI":"10.3390\/diagnostics12041008","article-title":"Urine flow cytometry parameter cannot safely predict contamination of urine\u2014a cohort study of a Swiss emergency department using machine learning techniques","volume":"12","author":"M\u00fcller","year":"2022","journal-title":"Diagnostics"},{"key":"B29","first-page":"113","article-title":"Ph value and antibacterial effect of alkasite restorative materials","author":"Obaees","year":"2024","journal-title":"Georgian Med. News"},{"key":"B30","doi-asserted-by":"publisher","DOI":"10.1128\/mra.01026-21","article-title":"De novo genome assembly of stinkhorn mushroom Clathrus columnatus (Basidiomycota, Fungi) using Illumina and Nanopore sequencing data","volume":"11","author":"Ogiso-Tanaka","year":"2022","journal-title":"Microbiol. Resour. Announc"},{"key":"B31","doi-asserted-by":"publisher","first-page":"1169","DOI":"10.1128\/aac.47.3.1169-1172.2003","article-title":"A new sulfonamide resistance gene (sul3) in Escherichia coli is widespread in the pig population of Switzerland","volume":"47","author":"Perreten","year":"2003","journal-title":"Antimicrob. Agents Chemother."},{"key":"B32","doi-asserted-by":"publisher","DOI":"10.1128\/mbio.02323-21","article-title":"A prospective, open-label pilot study of concurrent male partner treatment for bacterial vaginosis","volume":"12","author":"Plummer","year":"2021","journal-title":"mBio"},{"key":"B33","doi-asserted-by":"publisher","first-page":"119","DOI":"10.1016\/j.diagmicrobio.2010.12.002","article-title":"Multiplex PCR for detection of acquired carbapenemase genes","volume":"70","author":"Poirel","year":"2011","journal-title":"Diagn. Microbiol. Infect. Dis."},{"key":"B34","doi-asserted-by":"publisher","DOI":"10.14814\/phy2.15471","article-title":"Ammonium chloride\u2010induced acidosis exacerbates cystitis and pyelonephritis caused by uropathogenic E. coli","volume":"10","author":"Purkerson","year":"2022","journal-title":"Physiol. Rep."},{"key":"B35","doi-asserted-by":"publisher","first-page":"2031","DOI":"10.1039\/c9bm01785b","article-title":"Biomembrane induced in situ self-assembly of peptide with enhanced antimicrobial activity","volume":"8","author":"Shen","year":"2020","journal-title":"Biomater. Sci."},{"key":"B36","doi-asserted-by":"publisher","first-page":"982","DOI":"10.1107\/s0907444907036955","article-title":"Structure of GES-1 at atomic resolution: insights into the evolution of carbapenamase activity in the class A extended-spectrum \u03b2-lactamases","volume":"63","author":"Smith","year":"2007","journal-title":"Acta Crystallogr. D. Biol. Crystallogr."},{"key":"B37","doi-asserted-by":"publisher","first-page":"16","DOI":"10.37201\/req\/s02.02.2022","article-title":"Mechanism of action of cefiderocol","volume":"35","author":"Soriano","year":"2022","journal-title":"Rev. Esp Quimioter"},{"key":"B38","doi-asserted-by":"publisher","first-page":"191","DOI":"10.1007\/bf00339581","article-title":"Site-specific recombination promotes linkage between trimethoprim- and sulfonamide resistance genes. Sequence characterization of dhfrV and sulI and a recombination active locus of Tn21","volume":"213","author":"Sundstr\u00f6m","year":"1988","journal-title":"Mol. Gen. Genet."},{"key":"B39","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2016.00523","article-title":"Rapid identification and multiple susceptibility testing of pathogens from positive-culture sterile body fluids by a combined MALDI-TOF mass spectrometry and Vitek susceptibility system","volume":"7","author":"Tian","year":"2016","journal-title":"Front. Microbiol."},{"key":"B40","doi-asserted-by":"publisher","first-page":"169","DOI":"10.11604\/pamj.2020.36.169.21038","article-title":"Acute urinary tract infection in patients with underlying benign prostatic hyperplasia and prostate cancer","volume":"36","author":"Tolani","year":"2020","journal-title":"Pan Afr. Med. J."},{"key":"B41","article-title":"Mushroom toxicity","volume-title":"StatPearls","author":"Tran","year":"2023"},{"key":"B42","doi-asserted-by":"publisher","first-page":"967","DOI":"10.1007\/s40265-023-01893-6","article-title":"Extended and continuous infusion of novel protected \u03b2-lactam antibiotics: a narrative review","volume":"83","author":"Venuti","year":"2023","journal-title":"Drugs"},{"key":"B43","doi-asserted-by":"publisher","first-page":"147","DOI":"10.1128\/aac.35.1.147","article-title":"Transferable imipenem resistance in Pseudomonas aeruginosa","volume":"35","author":"Watanabe","year":"1991","journal-title":"Antimicrob. Agents Chemother."},{"key":"B44","doi-asserted-by":"publisher","first-page":"61","DOI":"10.1007\/s11255-022-03387-y","article-title":"Comparison potassium sodium hydrogen citrate with sodium bicarbonate in urine alkalization: a prospective crossover-controlled trial","volume":"55","author":"Xue","year":"2022","journal-title":"Int. Urol. Nephrol."}],"container-title":["Frontiers in Cellular and Infection Microbiology"],"original-title":[],"link":[{"URL":"https:\/\/www.frontiersin.org\/articles\/10.3389\/fcimb.2026.1647777\/full","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,30]],"date-time":"2026-04-30T05:40:33Z","timestamp":1777527633000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.frontiersin.org\/articles\/10.3389\/fcimb.2026.1647777\/full"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,4,30]]},"references-count":44,"alternative-id":["10.3389\/fcimb.2026.1647777"],"URL":"https:\/\/doi.org\/10.3389\/fcimb.2026.1647777","relation":{},"ISSN":["2235-2988"],"issn-type":[{"value":"2235-2988","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,4,30]]},"article-number":"1647777"}}