{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,11]],"date-time":"2026-03-11T03:07:46Z","timestamp":1773198466959,"version":"3.50.1"},"reference-count":36,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,3,10]],"date-time":"2026-03-10T00:00:00Z","timestamp":1773100800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Cell. Infect. Microbiol."],"abstract":"\n Objectives<\/jats:title>\n \n This study aimed to develop a microfluidic chip for the simultaneous detection of specific antibodies against six common pathogens in pastoral areas. The specific detection targets include: visceral leishmaniasis (IgG against\n Leishmania<\/jats:italic>\n soluble antigen, IgG against recombinant K39 antigen); cystic echinococcosis (IgG against\n Echinococcus<\/jats:italic>\n granulosus antigen 5, IgG against\n Echinococcus<\/jats:italic>\n granulosus antigen B); alveolar echinococcosis (IgG against\n Echinococcus<\/jats:italic>\n multilocularis antigen 2, IgG against\n Echinococcus<\/jats:italic>\n multilocularis antigen 18); brucellosis (IgG against\n Brucella<\/jats:italic>\n lipopolysaccharide, IgM against\n Brucella<\/jats:italic>\n lipopolysaccharide); Lyme disease (IgG against\n Borrelia<\/jats:italic>\n burgdorferi, IgM against\n Borrelia<\/jats:italic>\n burgdorferi); and Xinjiang hemorrhagic fever (virus-specific IgG, virus-specific IgM).\n <\/jats:p>\n <\/jats:sec>\n \n Methods<\/jats:title>\n \n Based on magnetic particle immunofluorescence, a multi-channel disc microfluidic chip and reagents were designed. Parameters (antigen-antibody microsphere ratio, sample dilution) were optimized; fluid dynamics tests verified fluid operation feasibility. Serum samples from patients with the six diseases and healthy controls were used to detect target antibodies. The chip\u2019s accuracy, dose-response curve (R\n 2<\/jats:sup>\n ), limit of detection (LOD), precision, and specificity were evaluated. Bland-Altman analysis compared results with traditional ELISA to assess consistency.\n <\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n \n The chip exhibited normal fluidic operation. Optimized reagents\/samples enhanced utilization efficiency. For the six diseases, the detection accuracy met the requirements: the coefficient of determination (R\n 2<\/jats:sup>\n ) for all indicators was greater than 0.98, the minimum limit of detection (LOD) among the 12 detection items was 0.6 \u03bcg\/mL, the relative standard deviation (RSD) for precision was less than 10%, and no cross-reactions occurred. Bland-Altman consistency analysis showed that the differences in detection results met clinically acceptable standards, demonstrating good consistency with enzyme-linked immunosorbent assay (ELISA).\n <\/jats:p>\n <\/jats:sec>\n \n Conclusions<\/jats:title>\n This chip exhibits stable fluid flow, low sample\/reagent consumption, and excellent accuracy\/precision\/specificity. Its detection results are consistent with those of traditional methods, and it enables the simultaneous combined detection of the aforementioned six infectious diseases. Compared with the clinically used method (enzyme-linked immunosorbent assay, ELISA), this chip has greater advantages in application scenarios and comprehensive benefits; compared with existing disc-based microfluidic technologies, it holds superior advantages in terms of detection throughput and application objectives. It provides a rapid, sensitive, and specific technical tool for the acute-phase screening, disease course confirmation, infection staging, differentiation between bacterial and parasitic infections, and disease prognosis detection of multiple prevalent infectious diseases in pastoral areas.<\/jats:p>\n <\/jats:sec>","DOI":"10.3389\/fcimb.2026.1693458","type":"journal-article","created":{"date-parts":[[2026,3,10]],"date-time":"2026-03-10T06:43:01Z","timestamp":1773124981000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["Application of multi-channel magnetic particle immunofluorescent disc microfluidic chip for combined detection of antibodies against six common infectious diseases including visceral leishmaniasis in pastoral areas"],"prefix":"10.3389","volume":"16","author":[{"given":"Yiran","family":"Wang","sequence":"first","affiliation":[{"name":"School of Clinical Medicine, Xinjiang Second Medical College","place":["Karamay, China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Junhao","family":"Li","sequence":"additional","affiliation":[{"name":"School of Public Health, Xinjiang Second Medical College","place":["Karamay, China"]}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1965","published-online":{"date-parts":[[2026,3,10]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"195","DOI":"10.1007\/s10393-024-01687-6","article-title":"Pastoralism and resulting challenges for national parks in afar, Ethiopia","volume":"21","author":"Abebe","year":"2024","journal-title":"Ecohealth"},{"key":"B2","doi-asserted-by":"publisher","DOI":"10.1016\/j.actatropica.2023.106925","article-title":"Past and present of diagnosis of echinococcosis: A review (1999-2021)","volume":"243","author":"Alvi","year":"2023","journal-title":"Acta Trop."},{"key":"B3","doi-asserted-by":"publisher","first-page":"11787","DOI":"10.1021\/acs.analchem.5c01324","article-title":"Diagnosis on-demand: field evaluation of microfluidic paper device for the detection of asymptomatic malaria","volume":"97","author":"Ayesha","year":"2025","journal-title":"Anal. 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