{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,4]],"date-time":"2026-06-04T06:04:37Z","timestamp":1780553077249,"version":"3.54.1"},"reference-count":35,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,6,4]],"date-time":"2026-06-04T00:00:00Z","timestamp":1780531200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Cell. Infect. Microbiol."],"abstract":"<jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>To investigate the antibacterial susceptibility and molecular characteristics of vancomycin resistant enterococci (VRE) isolated from 9 hospitals in Beijing from November 2021 to October 2022.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>The susceptibilities to 17 antibacteria agents were tested using two-fold agar dilution and broth microdilution. The resistance genes were characterized through whole genome sequence analysis. The bacteria typing was investigated through multilocus sequence typing (MLST), core genome MLST(cgMLST).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      A total of 251 VRE isolates including 247\n                      <jats:italic>Enterococcus faecium<\/jats:italic>\n                      (VREfm) and 4\n                      <jats:italic>Enterococcus faecalis<\/jats:italic>\n                      (VREfs) were collected in this study. More than 75% isolates were collected from urine. More than 89% isolates were resistant to teicoplanin. Besides glycopeptides, all the VRE isolates were resistant to penicillin, ampicillin and fluoroquinolones (VREfm) or erythromycin and fluoroquinolones (VREfs) and can be defined as multi-drug resistant. Among 247 VREfm, 72.9% (180\/247) carried\n                      <jats:italic>vanA<\/jats:italic>\n                      gene, 0.8% (2\/247) carried\n                      <jats:italic>vanM<\/jats:italic>\n                      gene, and 26.3% (65\/247) carried both\n                      <jats:italic>vanA<\/jats:italic>\n                      and\n                      <jats:italic>vanM<\/jats:italic>\n                      genes. A total of 22 sequence types (STs) were identified, with ST80 (24.7%, 61\/247) being the most predominant. cgMLST analysis revealed that ST80-CT8524 was the most common cluster among the VREfm isolates. All the 4 VREfs isolates harbored\n                      <jats:italic>vanA<\/jats:italic>\n                      gene and were assigned to 2 STs.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>\n                      VREfm isolates belonging to ST80 in Beijing was most common.\n                      <jats:italic>VanA<\/jats:italic>\n                      was still the predominant\n                      <jats:italic>van<\/jats:italic>\n                      gene and increased coexistence of\n                      <jats:italic>vanA<\/jats:italic>\n                      and\n                      <jats:italic>vanM<\/jats:italic>\n                      was noteworthy.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.3389\/fcimb.2026.1730782","type":"journal-article","created":{"date-parts":[[2026,6,4]],"date-time":"2026-06-04T05:35:21Z","timestamp":1780551321000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["Molecular epidemiology of vancomycin resistant enterococci collected from 9 hospitals in Beijing, China: a multicenter study"],"prefix":"10.3389","volume":"16","author":[{"given":"Lanqing","family":"Cui","sequence":"first","affiliation":[{"name":"Institute of Clinical Pharmacology, Peking University First Hospital","place":["Beijing, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yun","family":"Li","sequence":"additional","affiliation":[{"name":"Institute of Clinical Pharmacology, Peking University First Hospital","place":["Beijing, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lingyun","family":"Ma","sequence":"additional","affiliation":[{"name":"Institute of Clinical Pharmacology, Peking University First Hospital","place":["Beijing, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huimin","family":"Ma","sequence":"additional","affiliation":[{"name":"Institute of Clinical Pharmacology, Peking University First Hospital","place":["Beijing, China"]},{"name":"Department of Infectious Disease, Peking University First Hospital","place":["Beijing, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jingxian","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory, Aerospace Center Hospital","place":["Beijing, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanan","family":"Tan","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory, Beijing Haidian Hospital","place":["Beijing, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chunyue","family":"Ge","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory, Beijing Hospital, National Center for Gerontology; 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