{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,27]],"date-time":"2026-03-27T07:35:28Z","timestamp":1774596928179,"version":"3.50.1"},"reference-count":48,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,3,27]],"date-time":"2026-03-27T00:00:00Z","timestamp":1774569600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Cell. Infect. Microbiol."],"abstract":"<jats:sec>\n                    <jats:title>Introduction<\/jats:title>\n                    <jats:p>\n                      The growing prevalence of drug-resistant pathogens urgently calls for new treatment strategies. Traditional Chinese medicine (TCM) formulas, with their multi-targeted mechanisms of action, offer promising alternative options for antimicrobial therapy. This study aims to evaluate the antimicrobial activity of the TCM formula MangHuang solution (MH) after content detection of tannin, composed of\n                      <jats:italic>Rhei Radix et Rhizoma, Natrii Sulfas<\/jats:italic>\n                      , and\n                      <jats:italic>Galla Chinensis<\/jats:italic>\n                      , and to elucidate its antifungal mechanism against Candida albicans through integrated multi-omics analysis.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      MH and its individual or combined components were prepared and evaluated for their inhibitory effects on\n                      <jats:italic>Staphylococcus aureus, Escherichia coli<\/jats:italic>\n                      , and\n                      <jats:italic>Candida albicans<\/jats:italic>\n                      , and their antimicrobial activity was assessed. Transmission electron microscopy (TEM), biofilm formation experiments, and multi-omics analysis were used to investigate the antifungal mechanism of MH against\n                      <jats:italic>C. albicans<\/jats:italic>\n                      .\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>MH demonstrated potent and rapid antibacterial activity. Biofilm formation was significantly inhibited, manifested by reduced cell surface hydrophobicity, weakened initial adhesion capacity, and impaired biofilm maturation processes. Transcriptome and metabolome analyses revealed significant alterations in key metabolic pathways, particularly ABC transporters, amino acid biosynthesis, and protein-related pathways.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Discussion<\/jats:title>\n                    <jats:p>\n                      MH exhibits potent antifungal activity against\n                      <jats:italic>C. albicans<\/jats:italic>\n                      through a multi-target mechanism, primarily affecting biofilm formation and intracellular metabolic processes. The integration of multi-omics approaches provides strong evidence for the potential clinical application of MH as an effective antifungal agent.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.3389\/fcimb.2026.1737769","type":"journal-article","created":{"date-parts":[[2026,3,27]],"date-time":"2026-03-27T06:38:04Z","timestamp":1774593484000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["Integrated mechanistic and bioinformatics analysis of a traditional Chinese medicine compound MangHuang solution against Candida albicans"],"prefix":"10.3389","volume":"16","author":[{"given":"Hansheng","family":"Zhu","sequence":"first","affiliation":[{"name":"School of Pharmacy, Southwest Medical University","place":["Luzhou, China"]},{"name":"Key Laboratory of Biological Resource and Ecological Environment of Chinese Education Ministry, College of Life Sciences, Sichuan University","place":["Chengdu, China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peijian","family":"Ma","sequence":"additional","affiliation":[{"name":"School of Pharmacy, Southwest Medical University","place":["Luzhou, China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yefei","family":"Yuan","sequence":"additional","affiliation":[{"name":"School of Pharmacy, Southwest Medical University","place":["Luzhou, China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yiping","family":"Chen","sequence":"additional","affiliation":[{"name":"Key Laboratory of Biological Resource and Ecological Environment of Chinese Education Ministry, College of Life Sciences, Sichuan University","place":["Chengdu, China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hao","family":"Shi","sequence":"additional","affiliation":[{"name":"Key Laboratory of Biological Resource and Ecological Environment of Chinese Education Ministry, College of Life Sciences, Sichuan University","place":["Chengdu, China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Changyi","family":"Huang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Biological Resource and Ecological Environment of Chinese Education Ministry, College of Life Sciences, Sichuan University","place":["Chengdu, China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yue","family":"Zhou","sequence":"additional","affiliation":[{"name":"Key Laboratory of Biological Resource and Ecological Environment of Chinese Education Ministry, College of Life Sciences, Sichuan University","place":["Chengdu, China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Renzhi","family":"Jing","sequence":"additional","affiliation":[{"name":"R&D Department, Sichuan Golden Stone Hanfang Biotechnology Co., Ltd","place":["Chengdu, China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mei","family":"Cao","sequence":"additional","affiliation":[{"name":"Core Laboratory, School of Medicine, Sichuan Provincial People\u2019s Hospital Affiliated to University of Electronic Science and Technology of China","place":["Chengdu, China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dan","family":"Zhang","sequence":"additional","affiliation":[{"name":"School of Pharmacy, Southwest Medical University","place":["Luzhou, China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yu","family":"Luo","sequence":"additional","affiliation":[{"name":"School of Pharmacy, Southwest Medical University","place":["Luzhou, China"]}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1965","published-online":{"date-parts":[[2026,3,27]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"677","DOI":"10.3390\/toxins13100677","article-title":"Staphylococcus aureus toxins: An update on their pathogenic properties and potential treatments","volume":"13","author":"Ahmad-Mansour","year":"2021","journal-title":"Toxins"},{"key":"B2","doi-asserted-by":"publisher","first-page":"211","DOI":"10.2217\/fmb-2020-0059","article-title":"Different expression levels of ALS and SAP genes contribute to recurrent vulvovaginal candidiasis by Candida albicans","volume":"16","author":"Bonfim-Mendon\u00e7a","year":"2021","journal-title":"Future Microbiol."},{"key":"B3","doi-asserted-by":"publisher","first-page":"321","DOI":"10.2174\/1573411015666181204115035","article-title":"Miniaturized spectrophotometric method for quantification of tannins in pomegranate (Punica granatum L.) fruit peel dried extracts","volume":"16","author":"Br\u00eatas","year":"2020","journal-title":"Curr. 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