{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,14]],"date-time":"2026-07-14T12:25:21Z","timestamp":1784031921453,"version":"3.55.0"},"reference-count":97,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,3,18]],"date-time":"2026-03-18T00:00:00Z","timestamp":1773792000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100007061","name":"Secretar\u00eda Nacional de Ciencia, Tecnolog\u00eda e Innovaci\u00f3n","doi-asserted-by":"publisher","award":["PICT  PICT 2021-098, PICT 2020-0862, PIP 2021-3121, PUE 22920170100106CO."],"award-info":[{"award-number":["PICT  PICT 2021-098, PICT 2020-0862, PIP 2021-3121, PUE 22920170100106CO."]}],"id":[{"id":"10.13039\/501100007061","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Cell. Infect. Microbiol."],"abstract":"<jats:p>\n                    Chagas disease, caused by\n                    <jats:italic>Trypanosoma cruzi<\/jats:italic>\n                    , affects over seven million people worldwide. Vertical transmission during pregnancy contributes to the urban spread of the disease, including in non-endemic regions. Although the placenta constitutes a critical barrier against fetal infection, the molecular mechanisms underlying congenital transmission remain poorly understood. To identify placental factors associated with transmission, we performed a transcriptomic analysis of placental tissues from deliveries of congenitally infected (M+B+), exposed but uninfected (M+B\u2212), and unexposed\/uninfected (M\u2212B\u2212) newborns. Differential gene expression analysis comparing M+B+ and M\u2212B\u2212 placentas revealed overexpression of CEMIP (cell migration\u2013inducing hyaluronidase 1), involved in extracellular matrix (ECM) remodeling and intracellular transport, together with ENSG00000304767, a novel long non-coding RNA located intronically within\n                    <jats:italic>CEMIP<\/jats:italic>\n                    . In contrast, PRRX1 (paired related homeobox 1), CADM3 (cell adhesion molecule 3), and CDH11 (cadherin 11), genes associated with transcriptional regulation and cell\u2013cell adhesion, were underexpressed. In the M+B\u2212 versus M\u2212B\u2212 comparison, MIR4300HG, a long non-coding RNA hosting\n                    <jats:italic>MIR4300<\/jats:italic>\n                    , was overexpressed, whereas CGB5 (chorionic gonadotropin subunit beta 5), essential for pregnancy maintenance, was underexpressed. Direct comparison between M+B+ and M+B\u2212 placentas showed overexpression of the\n                    <jats:italic>CEMIP<\/jats:italic>\n                    -associated lncRNA and CGB5, accompanied by downregulation of CADM3, NEGR1, PDPN, and CDH11, implicating altered adhesion and structural pathways in transmission. Overall, these findings indicate that placental cell adhesion and ECM integrity are disrupted in transmitting placentas. Gene set enrichment analysis using the Gene Ontology library revealed alterations of immune-related pathways in both infected mother groups, while highlighting ECM-related processes\u2014particularly collagen organization and metabolism\u2014as key contributors to transmission events. Cell type enrichment analysis showed overrepresentation of extravillous trophoblasts in M+B+ placentas, with the opposite pattern observed in M+B\u2212 cases. Conversely, syncytiotrophoblasts and villous cytotrophoblasts were enriched in non-transmitting placentas relative to controls. Immune-associated placental cell types were consistently reduced in both infected groups. Co-expression network analysis further confirmed compromised placental signaling and structural integrity in transmitting cases, identifying ENPP1 and SLC16A10 as central hub genes. Together, these RNA-seq data define key placental transcriptional alterations associated with congenital\n                    <jats:italic>T. cruzi<\/jats:italic>\n                    transmission which, upon future experimental validation may provide insights into molecular mechanisms governing fetal protection or susceptibility.\n                  <\/jats:p>","DOI":"10.3389\/fcimb.2026.1749307","type":"journal-article","created":{"date-parts":[[2026,3,18]],"date-time":"2026-03-18T06:57:29Z","timestamp":1773817049000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":1,"title":["Placental transcriptome profiling in congenital Chagas disease: gene networks associated with transmission"],"prefix":"10.3389","volume":"16","author":[{"given":"Sofia","family":"Apodaca","sequence":"first","affiliation":[{"name":"Laboratorio de Biolog\u00eda Molecular de la Enfermedad de Chagas (LabMECh), Instituto de Investigaciones en Ingenier\u00eda Gen\u00e9tica y Biolog\u00eda Molecular (INGEBI), Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas (CONICET)","place":["Buenos Aires, Argentina"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emiliano E.","family":"Campos","sequence":"additional","affiliation":[{"name":"Unidad de Conocimiento Traslacional del Hospital P\u00fablico Materno Infantil (UCT-HPMI)","place":["Salta, Argentina"]},{"name":"Instituto de Patolog\u00eda Experimental, Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas (CONICET) - Universidad Nacional de Salta","place":["Salta, Argentina"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kevin D.","family":"Calupi\u00f1a","sequence":"additional","affiliation":[{"name":"Laboratorio de Gen\u00f3mica y Bioinform\u00e1tica de Pat\u00f3genos, iB3, Instituto de Biociencias, Biotecnolog\u00eda y Biolog\u00eda traslacional, Departamento de Fisiologia y Biologia Molecular y Celular, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires","place":["Buenos Aires, Argentina"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carolina","family":"Davies","sequence":"additional","affiliation":[{"name":"Instituto de Biolog\u00eda de Organismos Marinos (IBIOMAR), Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas (CONICET)","place":["Puerto Madryn, Chubut, Argentina"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ra\u00fal H.","family":"Lucero","sequence":"additional","affiliation":[{"name":"Instituto de Medicina Regional, Universidad Nacional del Nordeste (UNNE), Resistencia","place":["Chaco, Argentina"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Silvia A.","family":"Longhi","sequence":"additional","affiliation":[{"name":"Laboratorio de Biolog\u00eda Molecular de la Enfermedad de Chagas (LabMECh), Instituto de Investigaciones en Ingenier\u00eda Gen\u00e9tica y Biolog\u00eda Molecular (INGEBI), Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas (CONICET)","place":["Buenos Aires, Argentina"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laura","family":"Kamenetzky","sequence":"additional","affiliation":[{"name":"Laboratorio de Gen\u00f3mica y Bioinform\u00e1tica de Pat\u00f3genos, iB3, Instituto de Biociencias, Biotecnolog\u00eda y Biolog\u00eda traslacional, Departamento de Fisiologia y Biologia Molecular y Celular, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires","place":["Buenos Aires, Argentina"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria Paola","family":"Zago","sequence":"additional","affiliation":[{"name":"Unidad de Conocimiento Traslacional del Hospital P\u00fablico Materno Infantil (UCT-HPMI)","place":["Salta, Argentina"]},{"name":"Instituto de Patolog\u00eda Experimental, Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas (CONICET) - Universidad Nacional de Salta","place":["Salta, Argentina"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alejandro G.","family":"Schijman","sequence":"additional","affiliation":[{"name":"Laboratorio de Biolog\u00eda Molecular de la Enfermedad de Chagas (LabMECh), Instituto de Investigaciones en Ingenier\u00eda Gen\u00e9tica y Biolog\u00eda Molecular (INGEBI), Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas (CONICET)","place":["Buenos Aires, Argentina"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2026,3,18]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"431","DOI":"10.1093\/biolre\/ioad179","article-title":"Long non-coding RNAs: a summary of their roles in placenta development and pathology\u2020","volume":"110","author":"Adu-Gyamfi","year":"2024","journal-title":"Biol. 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