{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T19:24:29Z","timestamp":1789759469895,"version":"4.0.1"},"reference-count":32,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,2,2]],"date-time":"2026-02-02T00:00:00Z","timestamp":1769990400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100002858","name":"China Postdoctoral Science Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002858","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Cell. Infect. Microbiol."],"abstract":"<jats:p>\n                    Platelet-rich plasma (PRP) is known as an autologous biological agent that can immensely promote wound healing. Bacterial infection is a common complication of wounds which obstructs wound healing. However, the effect of PRP on bacteria is still inconclusive. This\n                    <jats:italic>in vitro<\/jats:italic>\n                    study evaluated the antibacterial activity of PRP, platelet-poor plasma (PPP) and Frozen-PRP (Fro-PRP) against\n                    <jats:italic>Escherichia coli<\/jats:italic>\n                    ,\n                    <jats:italic>Klebsiella pneumoniae<\/jats:italic>\n                    ,\n                    <jats:italic>Enterobacter cloacae<\/jats:italic>\n                    ,\n                    <jats:italic>Staphylococcus aureus<\/jats:italic>\n                    , and\n                    <jats:italic>Enterococcus faecalis<\/jats:italic>\n                    . The antimicrobial activity was performed using modified agar diffusion method, colony-forming units counting test, and bacterial growth curve analysis. The results showed that PRP, PPP and Fro-PRP had antimicrobial effects on five bacterial strains including\n                    <jats:italic>Escherichia coli<\/jats:italic>\n                    ,\n                    <jats:italic>Klebsiella pneumoniae<\/jats:italic>\n                    ,\n                    <jats:italic>Enterobacter cloacae<\/jats:italic>\n                    ,\n                    <jats:italic>Staphylococcus aureus<\/jats:italic>\n                    , and\n                    <jats:italic>Enterococcus faecalis<\/jats:italic>\n                    . The antimicrobial activity of PRP was significantly stronger than that of Fro-PRP and PPP. To a certain extent, frozen storage may reduce the antibacterial ability of PRP. Overall, our study demonstrates that PRP and other platelet derived products seem to be a promising alternative tool for infected wounds treatment.\n                  <\/jats:p>","DOI":"10.3389\/fcimb.2026.1757780","type":"journal-article","created":{"date-parts":[[2026,2,2]],"date-time":"2026-02-02T06:32:10Z","timestamp":1770013930000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":2,"title":["In vitro antibacterial activity of platelet-rich plasma against Gram-positive and Gram-negative pathogens"],"prefix":"10.3389","volume":"16","author":[{"given":"Yan","family":"Liu","sequence":"first","affiliation":[{"name":"Department of Clinical Laboratory, Maternal and Child Health Hospital of Hubei Province","place":["Wuhan, Hubei, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lei","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Transfusion, General Hospital of Central Theater Command","place":["Wuhan, Hubei, China"]},{"name":"Medical College, Wuhan University of Science and Technology","place":["Wuhan, Hubei, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jinbing","family":"Du","sequence":"additional","affiliation":[{"name":"Department of Transfusion, General Hospital of Central Theater Command","place":["Wuhan, Hubei, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenxu","family":"Ni","sequence":"additional","affiliation":[{"name":"Department of Transfusion, General Hospital of Central Theater Command","place":["Wuhan, Hubei, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tingting","family":"Zou","sequence":"additional","affiliation":[{"name":"Department of Transfusion, General Hospital of Central Theater Command","place":["Wuhan, Hubei, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zihan","family":"Yuan","sequence":"additional","affiliation":[{"name":"Department of Transfusion, General Hospital of Central Theater Command","place":["Wuhan, Hubei, China"]},{"name":"Medical College, Wuhan University of Science and Technology","place":["Wuhan, Hubei, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yingkai","family":"Xu","sequence":"additional","affiliation":[{"name":"Department of Transfusion, General Hospital of Central Theater Command","place":["Wuhan, Hubei, China"]},{"name":"Medical College, Wuhan University of Science and Technology","place":["Wuhan, Hubei, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Junying","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Transfusion, General Hospital of Central Theater Command","place":["Wuhan, Hubei, China"]},{"name":"Medical College, Wuhan University of Science and Technology","place":["Wuhan, Hubei, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mengyu","family":"Shen","sequence":"additional","affiliation":[{"name":"Basic Medical Laboratory, General Hospital of Central Theater Command","place":["Wuhan, Hubei, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yong","family":"Qi","sequence":"additional","affiliation":[{"name":"Department of Transfusion, General Hospital of Central Theater Command","place":["Wuhan, Hubei, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wanbing","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Transfusion, General Hospital of Central Theater Command","place":["Wuhan, Hubei, China"]},{"name":"Medical College, Wuhan University of Science and Technology","place":["Wuhan, Hubei, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2026,2,2]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"15145","DOI":"10.1038\/s41598-025-97418-0","article-title":"Platelet-rich plasma as a potential antimicrobial agent against multidrug-resistant bacteria in diabetic foot infections","volume":"15","author":"Aboelsaad","year":"2025","journal-title":"Sci. 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