{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,19]],"date-time":"2026-06-19T05:54:48Z","timestamp":1781848488029,"version":"3.54.5"},"reference-count":46,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,6,19]],"date-time":"2026-06-19T00:00:00Z","timestamp":1781827200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Cell. Infect. Microbiol."],"abstract":"<jats:p>\n                    Tuberculosis (TB) is a severe zoonotic disease that represents a considerable threat to the health of captive non-human primates (NHPs). Conventional serological diagnostic methods often suffer from insufficient sensitivity and specificity, making them difficult to meet the requirements of large-scale screening in monkey colonies. In this study, we predicted and screened Mycobacterium tuberculosis antigenic epitopes using immunoinformatic approaches, and identified a novel antigenic peptide MP71 from the MPT64 protein. Based on the MP71-KLH conjugate, we further developed an indirect ELISA assay. In both the learning cohort (\n                    <jats:italic>n<\/jats:italic>\n                    = 19) and independent validation cohort (\n                    <jats:italic>n<\/jats:italic>\n                    = 21), the assay exhibited favorable and stable diagnostic performance: in the validation cohort, the AUC was &amp;gt;0.94 (95% CI: 0.8471 to 1.000), with a sensitivity of &amp;gt;92% and a specificity of 100%, together with favorable repeatability. The established ELISA represents a rapid, simple, and reliable serological tool that can be used for routine surveillance and large-scale preliminary screening of tuberculosis in monkeys.\n                  <\/jats:p>","DOI":"10.3389\/fcimb.2026.1799877","type":"journal-article","created":{"date-parts":[[2026,6,19]],"date-time":"2026-06-19T05:47:34Z","timestamp":1781848054000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["From immunoinformatics insights to assay development: establishment of a peptide-based indirect elisa for cynomolgus tuberculosis detection"],"prefix":"10.3389","volume":"16","author":[{"given":"Dengling","family":"Huang","sequence":"first","affiliation":[{"name":"Nanjing University of Chinese Medicine","place":["Nanjing, China"]},{"name":"Zhongshan Institute for Drug Discovery, Shanghai Institute of Materia Medica, Chinese Academy of Sciences","place":["Zhongshan, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mingjie","family":"Chen","sequence":"additional","affiliation":[{"name":"School of Pharmaceutical Sciences, Southern Medical University","place":["Guangzhou, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qiuyu","family":"Wu","sequence":"additional","affiliation":[{"name":"Nanjing University of Chinese Medicine","place":["Nanjing, China"]},{"name":"Zhongshan Institute for Drug Discovery, Shanghai Institute of Materia Medica, Chinese Academy of Sciences","place":["Zhongshan, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pengwei","family":"Liu","sequence":"additional","affiliation":[{"name":"Zhongshan Institute for Drug Discovery, Shanghai Institute of Materia Medica, Chinese Academy of Sciences","place":["Zhongshan, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leting","family":"Yang","sequence":"additional","affiliation":[{"name":"Zhongshan Institute for Drug Discovery, Shanghai Institute of Materia Medica, Chinese Academy of Sciences","place":["Zhongshan, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Weixiang","family":"Huang","sequence":"additional","affiliation":[{"name":"Zhongshan Institute for Drug Discovery, Shanghai Institute of Materia Medica, Chinese Academy of Sciences","place":["Zhongshan, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kunhui","family":"Mo","sequence":"additional","affiliation":[{"name":"Zhongshan Institute for Drug Discovery, Shanghai Institute of Materia Medica, Chinese Academy of Sciences","place":["Zhongshan, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiao","family":"Wei","sequence":"additional","affiliation":[{"name":"Guangxi Zhuang Autonomous Region Terrestrial Wildlife Medical-aid and Monitoring Epidemic Diseases Research Center","place":["Nanning, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ying","family":"Tian","sequence":"additional","affiliation":[{"name":"Zhongshan Institute for Drug Discovery, Shanghai Institute of Materia Medica, Chinese Academy of Sciences","place":["Zhongshan, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianhua","family":"Sun","sequence":"additional","affiliation":[{"name":"Nanjing University of Chinese Medicine","place":["Nanjing, China"]},{"name":"Zhongshan Institute for Drug Discovery, Shanghai Institute of Materia Medica, Chinese Academy of Sciences","place":["Zhongshan, China"]},{"name":"State Key Laboratory of Drug Research, Shanghai Institute of Materia Medica, Chinese Academy of Sciences","place":["Shanghai, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Likun","family":"Gong","sequence":"additional","affiliation":[{"name":"Nanjing University of Chinese Medicine","place":["Nanjing, China"]},{"name":"Zhongshan Institute for Drug Discovery, Shanghai Institute of Materia Medica, Chinese Academy of Sciences","place":["Zhongshan, China"]},{"name":"School of Pharmaceutical Sciences, Southern Medical University","place":["Guangzhou, China"]},{"name":"State Key Laboratory of Drug Research, Shanghai Institute of Materia Medica, Chinese Academy of Sciences","place":["Shanghai, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2026,6,19]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"493","DOI":"10.1038\/s41586-024-07487-w","article-title":"Accurate structure prediction of biomolecular interactions with AlphaFold 3","volume":"630","author":"Abramson","year":"2024","journal-title":"Nature"},{"key":"B2","doi-asserted-by":"publisher","first-page":"4065","DOI":"10.1007\/s11033-020-05413-7","article-title":"Advances in diagnosis of tuberculosis: an update into molecular diagnosis of Mycobacterium tuberculosis","volume":"47","author":"Acharya","year":"2020","journal-title":"Mol. 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