{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,20]],"date-time":"2026-10-20T01:02:08Z","timestamp":1792458128645,"version":"4.5.0"},"reference-count":52,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T00:00:00Z","timestamp":1777420800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Cell. Infect. Microbiol."],"abstract":"<jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>\n                      Robot-assisted gait training (RAGT) improves motor recovery after stroke, yet its biological mechanisms remain poorly understood. Increasing evidence suggests that gut microbiota dysbiosis contributes to stroke pathology and recovery.\n                      <jats:italic>Objective<\/jats:italic>\n                      . To investigate whether RAGT alleviates acute ischemic stroke (AIS) symptoms by modulating gut microbiota composition and function.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      Forty-seven newly diagnosed AIS patients were enrolled and assigned to a RAGT group (IG,\n                      <jats:italic>n<\/jats:italic>\n                      \u00a0=\u00a024) or a conventional gait training group (CG,\n                      <jats:italic>n<\/jats:italic>\n                      \u00a0=\u00a023). Fecal samples were collected after the intervention, and functional outcomes were assessed at 90 days. Gut microbiota composition and predicted function were analyzed using 16S rRNA gene sequencing.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Compared with CG patients, IG patients demonstrated significantly improved clinical outcomes. RAGT partially reversed stroke-associated gut dysbiosis, characterized by increased microbial richness and evenness. Beneficial taxa, including\n                      <jats:italic>Lachnospiraceae<\/jats:italic>\n                      ,\n                      <jats:italic>Ruminococcaceae<\/jats:italic>\n                      , and\n                      <jats:italic>Oscillospiraceae<\/jats:italic>\n                      , were enriched, whereas\n                      <jats:italic>Enterobacteriaceae<\/jats:italic>\n                      and\n                      <jats:italic>Bifidobacteriaceae<\/jats:italic>\n                      were reduced. Functional prediction revealed decreased pathogenic potential of the gut microbiota in the IG, which correlated with improved AIS outcomes.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Poststroke RAGT was associated with alteration of gut microbiota composition and function, suggesting a novel microbiota-mediated mechanism underlying its neurorehabilitative benefits in AIS.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.3389\/fcimb.2026.1810501","type":"journal-article","created":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T05:57:59Z","timestamp":1777442279000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":2,"title":["Robot-assisted gait training alleviates the symptoms of acute ischemic stroke via regulating gut microbiota composition and 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