{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T04:52:03Z","timestamp":1791348723600,"version":"4.3.1"},"reference-count":21,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T00:00:00Z","timestamp":1791331200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100018370","name":"Instituut voor Tropische Geneeskunde","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100018370","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Cell. Infect. Microbiol."],"abstract":"\n Introduction<\/jats:title>\n Molecular diagnostics have improved infectious disease detection, yet their role in parasitology remains nuanced.<\/jats:p>\n <\/jats:sec>\n \n Methods<\/jats:title>\n \n We compared the performance of the AMPLIQUICK\n \u00ae<\/jats:sup>\n Fecal Parasitology assay with the conventional methods encompassing microscopy, antigen testing and in-house PCR testing, in a reference laboratory for parasitology in a travel clinic. The detection of protozoa, helminths, and microsporidia was evaluated on 107 stool samples from 99 patients with suspected gastrointestinal parasitic infections, of which 40 specimens were tested retrospectively and 67 were collected prospectively.\n <\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n \n AMPLIQUICK\n \u00ae<\/jats:sup>\n demonstrated high accuracy for protozoa and microsporidia (94.6%, 35\/37) detecting all\n Entamoeba histolytica<\/jats:italic>\n ,\n Entamoeba dispar<\/jats:italic>\n , most\n Giardia duodenalis<\/jats:italic>\n and\n Cryptosporidium<\/jats:italic>\n spp., and all\n Enterocytozoon bieneusi<\/jats:italic>\n and\n Encephalitozoon<\/jats:italic>\n spp. The assay detected more\n Dientamoeba fragilis<\/jats:italic>\n (24 vs. 12) and\n Blastocystis<\/jats:italic>\n sp. (61 vs. 44) than the conventional method but the correctness of these results could not be established. AMPLIQUICK\n \u00ae<\/jats:sup>\n reliably detected most clinically relevant helminths, including\n Ascaris lumbricoides<\/jats:italic>\n ,\n Strongyloides<\/jats:italic>\n spp.,\n Hymenolepis nana<\/jats:italic>\n ,\n Taenia<\/jats:italic>\n spp., and\n Enterobius vermicularis.<\/jats:italic>\n Hookworms,\n Trichuris trichiura<\/jats:italic>\n ,\n Schistosoma mansoni<\/jats:italic>\n ,\n Heterophyidae\/Opisthorchidae<\/jats:italic>\n and\n Trichostrongylus<\/jats:italic>\n spp. were occasionally missed.\n <\/jats:p>\n <\/jats:sec>\n \n Discussion<\/jats:title>\n \n AMPLIQUICK\n \u00ae<\/jats:sup>\n demonstrated good performance compared to the conventional methods and has the potential to improve parasitology diagnostics, particularly in laboratories with limited expertise. Clinicians should interpret findings in the patient\u2019s clinical context.\n <\/jats:p>\n <\/jats:sec>","DOI":"10.3389\/fcimb.2026.1816447","type":"journal-article","created":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T04:49:21Z","timestamp":1791348561000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["Evaluation of the Biosynex AMPLIQUICK\u00ae Fecal Parasitology assays in a Belgian travel clinic"],"prefix":"10.3389","volume":"16","author":[{"given":"Jasmine","family":"Coppens","sequence":"first","affiliation":[{"name":"Department of Clinical Sciences, Institute of Tropical Medicine, Nationalestraat","place":["Antwerp, Belgium"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean-Marc","family":"Schwob","sequence":"additional","affiliation":[{"name":"Department of Clinical Sciences, Institute of Tropical Medicine, Nationalestraat","place":["Antwerp, Belgium"]},{"name":"Division of Tropical and Humanitarian Medicine, Geneva University Hospitals","place":["Geneva, Switzerland"]},{"name":"Clinical Microbiology Laboratory, Geneva University Hospitals","place":["Geneva, Switzerland"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karin","family":"Van Looveren","sequence":"additional","affiliation":[{"name":"Department of Clinical Sciences, Institute of Tropical Medicine, Nationalestraat","place":["Antwerp, Belgium"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jolien","family":"Baeyens","sequence":"additional","affiliation":[{"name":"Department of Clinical Sciences, Institute of Tropical Medicine, Nationalestraat","place":["Antwerp, Belgium"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marjan","family":"Van Esbroeck","sequence":"additional","affiliation":[{"name":"Department of Clinical Sciences, Institute of Tropical Medicine, Nationalestraat","place":["Antwerp, Belgium"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2026,10,7]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","DOI":"10.3390\/genes13122387","article-title":"Real-time polymerase chain reaction: Current techniques, applications, and role in COVID-19 diagnosis","volume":"13","author":"Artika","year":"2022","journal-title":"Genes (Basel)."},{"key":"B2","doi-asserted-by":"publisher","first-page":"48","DOI":"10.1051\/parasite\/2018049","article-title":"Comparison of three commercial multiplex PCR assays for the diagnosis of intestinal protozoa","volume":"25","author":"Autier","year":"2018","journal-title":"Parasite."},{"key":"B3","doi-asserted-by":"publisher","DOI":"10.3390\/microorganisms8040569","article-title":"Evaluation of the AllplexTM gastrointestinal panel-parasite assay for protozoa detection in stool samples: A retrospective and prospective study","volume":"8","author":"Autier","year":"2020","journal-title":"Microorganisms"},{"key":"B4","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.00986-22","article-title":"Where have all the diagnostic morphological parasitologists gone","volume":"60","author":"Bradbury","year":"2022","journal-title":"J. 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