{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,8]],"date-time":"2026-05-08T06:16:16Z","timestamp":1778220976238,"version":"3.51.4"},"reference-count":29,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,5,8]],"date-time":"2026-05-08T00:00:00Z","timestamp":1778198400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Cell. Infect. Microbiol."],"abstract":"<jats:p>\n                    <jats:italic>Mycobacterium abscessus<\/jats:italic>\n                    (Mab) is an emerging pulmonary pathogen with extensive drug resistance, resulting in prolonged multidrug therapy, severe side effects, and cure rates below 50%. The absence of a reliable immunocompetent mouse model and the poor translation of\n                    <jats:italic>in vitro<\/jats:italic>\n                    drug activity to mammalian\n                    <jats:italic>in vivo<\/jats:italic>\n                    efficacy hinder preclinical progress. To address this gap, we developed a novel\n                    <jats:italic>Galleria mellonella<\/jats:italic>\n                    infection model for Mab, combined with bioluminescence imaging (BLI), enabling longitudinal monitoring and quantification of mycobacterial burden. Two double-reporter strains were assessed: a firefly luciferase\n                    <jats:italic>-<\/jats:italic>\n                    expressing strain requiring exogenous luciferin, and an autonomously luminescent Lux strain. Both generated stable signals suitable for longitudinal\n                    <jats:italic>in vitro<\/jats:italic>\n                    and\n                    <jats:italic>in vivo<\/jats:italic>\n                    imaging, with improved\n                    <jats:italic>in vivo<\/jats:italic>\n                    detectability of the luciferase-expressing strain based on its characteristic red-shifted emission spectrum. BLI provided sensitive, early quantification of mycobacterial load and treatment responses, outperforming traditional larval endpoints. This model supports early detection of dose-dependent treatment effects and enables real-time monitoring, thereby highlighting the value of\n                    <jats:italic>G. mellonella<\/jats:italic>\n                    as a rapid, cost-effective complementary preclinical model for screening novel antimycobacterial compounds.\n                  <\/jats:p>","DOI":"10.3389\/fcimb.2026.1817296","type":"journal-article","created":{"date-parts":[[2026,5,8]],"date-time":"2026-05-08T05:52:13Z","timestamp":1778219533000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["Bioluminescent Mycobacterium abscessus in Galleria mellonella: a preclinical screening tool for antimycobacterial compounds"],"prefix":"10.3389","volume":"16","author":[{"given":"Tine","family":"Van Win","sequence":"first","affiliation":[{"name":"Laboratory of Clinical Microbiology, Department of Microbiology, Immunology and Transplantation, KU Leuven","place":["Leuven, Belgium"]},{"name":"Biomedical MRI, Department of Imaging and Pathology, KU Leuven","place":["Leuven, Belgium"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wannes","family":"Brangers","sequence":"additional","affiliation":[{"name":"Laboratory of Clinical Microbiology, Department of Microbiology, Immunology and Transplantation, KU Leuven","place":["Leuven, Belgium"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Agustin","family":"Res\u00e9ndiz Sharpe","sequence":"additional","affiliation":[{"name":"Biomedical MRI, Department of Imaging and Pathology, KU Leuven","place":["Leuven, Belgium"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gert-Jan","family":"Wijnant","sequence":"additional","affiliation":[{"name":"Laboratory of Clinical Microbiology, Department of Microbiology, Immunology and Transplantation, KU Leuven","place":["Leuven, Belgium"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Emmanuel","family":"Andr\u00e9","sequence":"additional","affiliation":[{"name":"Laboratory of Clinical Microbiology, Department of Microbiology, Immunology and Transplantation, KU Leuven","place":["Leuven, Belgium"]},{"name":"Department of Laboratory Medicine, UZ Leuven","place":["Leuven, Belgium"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Greetje","family":"Vande Velde","sequence":"additional","affiliation":[{"name":"Biomedical MRI, Department of Imaging and Pathology, KU Leuven","place":["Leuven, Belgium"]}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1965","published-online":{"date-parts":[[2026,5,8]]},"reference":[{"key":"B1","unstructured":"Andr\u00e9\n              E.\n            \n          \n          \n          2026"},{"key":"B2","doi-asserted-by":"publisher","first-page":"2","DOI":"10.1093\/femsre\/fuad011","article-title":"Galleria mellonella\u2013intracellular bacteria pathogen infection models: the ins and outs","volume":"47","author":"Asai","year":"2023","journal-title":"FEMS Microbiol. 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