{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,11]],"date-time":"2026-06-11T06:01:15Z","timestamp":1781157675367,"version":"3.54.1"},"reference-count":28,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,6,11]],"date-time":"2026-06-11T00:00:00Z","timestamp":1781136000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Cell. Infect. Microbiol."],"abstract":"<jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Ulcerative Cutaneous Tuberculosis (UCT) represents a diagnostically complex variant of extrapulmonary tuberculosis, characterized by the absence of standardized biomarkers for monitoring treatment efficacy.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>\n                      This study sought to explore the potential of lncRNA\n                      <jats:italic>HCP5<\/jats:italic>\n                      as a diagnostic and therapeutic biomarker for UCT.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      We conducted an integrated bioinformatics analysis on clinical samples from patients with UCT and Non-Tuberculous Cutaneous Ulcers to identify differentially expressed lncRNAs. Co-expression networks were developed to associate HCP5 with downstream mRNAs. Validation was performed using patient tissue samples and an\n                      <jats:italic>in vitro<\/jats:italic>\n                      model of BCG-infected macrophages, incorporating functional knockdown studies. Longitudinal profiling of UCT patients undergoing therapy was employed to evaluate the dynamics of the biomarker.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Our investigation identified 1,994 differentially expressed long non-coding RNAs (lncRNAs), with HCP5 being notably upregulated. Through co-expression analysis, HCP5 was linked to immune and inflammation pathways, and 12 correlated mRNAs were identified, among which ITGB2 was validated as a downstream target. The knockdown of HCP5 resulted in a significant reduction of ITGB2 expression in macrophages. Longitudinal analysis demonstrated a significant decline in both HCP5 and ITGB2 levels in patient tissues and plasma following two weeks of anti-tuberculosis treatment.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Discussion<\/jats:title>\n                    <jats:p>This study elucidates a novel HCP5\/ITGB2 regulatory axis in the pathogenesis of UCT and highlights their dynamic responsiveness to therapeutic intervention. These findings address a critical clinical gap by providing objective biomarkers for the diagnosis and monitoring of treatment efficacy in cutaneous tuberculosis.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.3389\/fcimb.2026.1824185","type":"journal-article","created":{"date-parts":[[2026,6,11]],"date-time":"2026-06-11T05:47:18Z","timestamp":1781156838000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["The role of long non-coding RNA HCP5 in ulcerative cutaneous tuberculosis therapy introduction"],"prefix":"10.3389","volume":"16","author":[{"given":"Jiayan","family":"Qian","sequence":"first","affiliation":[{"name":"Affiliated of Nanjing University of Chinese Medicine to Nanjing Integrated Traditional Chinese and Western Medicine Hospital","place":["Jiangsu, Nanjing, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiayue","family":"Sun","sequence":"additional","affiliation":[{"name":"Jiangsu Second Chinese Medicine Hospital","place":["Jiangsu, Nanjing, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ye","family":"Liu","sequence":"additional","affiliation":[{"name":"Affiliated of Nanjing University of Chinese Medicine to Nanjing Integrated Traditional Chinese and Western Medicine Hospital","place":["Jiangsu, Nanjing, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qianqian","family":"Bu","sequence":"additional","affiliation":[{"name":"Affiliated of Nanjing University of Chinese Medicine to Nanjing Integrated Traditional Chinese and Western Medicine Hospital","place":["Jiangsu, Nanjing, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tingjun","family":"Cao","sequence":"additional","affiliation":[{"name":"Affiliated of Nanjing University of Chinese Medicine to Nanjing Integrated Traditional Chinese and Western Medicine Hospital","place":["Jiangsu, Nanjing, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Quanyong","family":"Jin","sequence":"additional","affiliation":[{"name":"Affiliated of Nanjing University of Chinese Medicine to Nanjing Integrated Traditional Chinese and Western Medicine Hospital","place":["Jiangsu, Nanjing, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liangjie","family":"Fu","sequence":"additional","affiliation":[{"name":"Affiliated of Nanjing University of Chinese Medicine to Nanjing Integrated Traditional Chinese and Western Medicine Hospital","place":["Jiangsu, Nanjing, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuling","family":"Wang","sequence":"additional","affiliation":[{"name":"Affiliated of Nanjing University of Chinese Medicine to Nanjing Integrated Traditional Chinese and Western Medicine Hospital","place":["Jiangsu, Nanjing, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xianyu","family":"Gong","sequence":"additional","affiliation":[{"name":"Maqun Community Health Service Center","place":["Jiangsu, Nanjing, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rui","family":"Deng","sequence":"additional","affiliation":[{"name":"Affiliated of Nanjing University of Chinese Medicine to Nanjing Integrated Traditional Chinese and Western Medicine Hospital","place":["Jiangsu, Nanjing, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zihui","family":"Huang","sequence":"additional","affiliation":[{"name":"Affiliated of Nanjing University of Chinese Medicine to Nanjing Integrated Traditional Chinese and Western Medicine Hospital","place":["Jiangsu, Nanjing, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2026,6,11]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","DOI":"10.1016\/j.intimp.2025.114967","article-title":"Mycobacterium tuberculosis stimulates cuproptosis by regulating Lnc-Gm5532 to target FDX1 for bacteria intracellular survival","volume":"160","author":"Gong","year":"2025","journal-title":"Int. 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