{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,15]],"date-time":"2026-06-15T05:55:03Z","timestamp":1781502903834,"version":"3.54.1"},"reference-count":37,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,6,15]],"date-time":"2026-06-15T00:00:00Z","timestamp":1781481600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Cell. Infect. Microbiol."],"abstract":"\n Background<\/jats:title>\n This study aimed to identify the microbiota and specific genes that are closely associated with colorectal cancer (CRC) through metagenomic sequencing and integrative multi-omics analysis.<\/jats:p>\n <\/jats:sec>\n \n Methods<\/jats:title>\n Fecal samples were collected from 11 healthy volunteers and 20 patients with CRC. Genomic DNA was extracted for metagenomic analysis and high-throughput sequencing. Compositional differences and correlations of the gut microbiome were compared based on species and functional diversity.<\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n \n The overall species composition included 1,980 species, with 1,707 species identified in the CRC group and 1,525 in the healthy control group. Alpha diversity was significantly lower in the CRC group than in the healthy control group (\n p<\/jats:italic>\n \u00a0=\u00a00.014). Beta diversity analysis revealed significant differences between the two groups (stress = 0.1308,\n p<\/jats:italic>\n \u00a0=\u00a00.021). Based on LEfSe analysis,\n Shigella<\/jats:italic>\n ,\n Porphyromonas<\/jats:italic>\n ,\n Proteus<\/jats:italic>\n ,\n Bacteroides<\/jats:italic>\n ,\n Alistipes<\/jats:italic>\n ,\n Fusobacterium<\/jats:italic>\n , and\n Escherichia<\/jats:italic>\n were more abundant in patients with CRC, whereas\n Eubacterium<\/jats:italic>\n ,\n Clostridium<\/jats:italic>\n ,\n Dialister<\/jats:italic>\n ,\n Faecalibacterium<\/jats:italic>\n ,\n Blautia<\/jats:italic>\n ,\n Coprococcus<\/jats:italic>\n ,\n Dorea<\/jats:italic>\n ,\n Subdoligranulum<\/jats:italic>\n ,\n Megamonas<\/jats:italic>\n ,\n Roseburia<\/jats:italic>\n ,\n and Prevotella<\/jats:italic>\n were significantly more abundant in the healthy control group (\n p<\/jats:italic>\n \u00a0&lt;\u00a00.05).\n <\/jats:p>\n <\/jats:sec>\n \n Conclusion<\/jats:title>\n A multidimensional microbial diagnostic model, incorporating Shigella, Porphyromonas, Proteus, Bacteroides, Fusobacterium, Escherichia, Eubacterium, Clostridium, Dialister, Faecalibacterium, Blautia, Coprococcus, Dorea, Subdoligranulum, Megamonas, Roseburia, and Prevotella, suggests the potential to enhance early CRC screening performance. Furthermore, LptA, tnaA, envC, and argB may represent promising candidates for novel therapeutic targets, warranting further investigation.<\/jats:p>\n <\/jats:sec>","DOI":"10.3389\/fcimb.2026.1828012","type":"journal-article","created":{"date-parts":[[2026,6,15]],"date-time":"2026-06-15T05:33:03Z","timestamp":1781501583000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["Metagenomic analysis of human feces reveals gut microbiome role in colorectal cancer"],"prefix":"10.3389","volume":"16","author":[{"given":"Zehui","family":"Gu","sequence":"first","affiliation":[{"name":"Department of Pathology, The Third Affiliated Hospital of Jinzhou Medical University","place":["Jinzhou, Liaoning, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qi","family":"Tan","sequence":"additional","affiliation":[{"name":"Department of Pathology, The Third Affiliated Hospital of Jinzhou Medical University","place":["Jinzhou, Liaoning, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dong","family":"Mao","sequence":"additional","affiliation":[{"name":"Department of General Surgery, The First Affiliated Hospital of Jinzhou Medical University","place":["Jinzhou, Liaoning, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yun","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology, The First Affiliated Hospital of Jinzhou Medical University","place":["Jinzhou, Liaoning, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yadi","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Precision Medicine, The Third Affiliated Hospital of Jinzhou Medical University","place":["Jinzhou, Liaoning, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dongning","family":"He","sequence":"additional","affiliation":[{"name":"Department of Oncology, The Third Affiliated Hospital of Jinzhou Medical University","place":["Jinzhou, Liaoning, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Suxian","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Pathology, The Third Affiliated Hospital of Jinzhou Medical University","place":["Jinzhou, Liaoning, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2026,6,15]]},"reference":[{"key":"B3","doi-asserted-by":"publisher","DOI":"10.7759\/cureus.73302","article-title":"Evaluation of morbidity and mortality in iatrogenic colonic perforation during colonoscopy: a comprehensive systematic review and meta-analysis","volume":"16","author":"Adebisi","year":"2024","journal-title":"Cureus"},{"key":"B13","doi-asserted-by":"publisher","first-page":"72","DOI":"10.1186\/s13099-024-00661-5","article-title":"Investigation of gut microbiota composition in humans carrying blastocystis subtypes 1 and 2 and Entamoeba hartmanni","volume":"16","author":"Antonetti","year":"2024","journal-title":"Gut Pathog."},{"key":"B35","doi-asserted-by":"publisher","DOI":"10.3390\/microorganisms9102156","article-title":"Diversity of the tryptophanase gene and its evolutionary implications in living organisms","volume":"9","author":"Boya","year":"2021","journal-title":"Microorganisms"},{"key":"B24","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2021.712028","article-title":"The effects of Chinese medicine QRD, antibiotics, and probiotics on therapy and gut microbiota in septic rats","volume":"11","author":"Cao","year":"2021","journal-title":"Front. 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