{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,11]],"date-time":"2026-09-11T05:08:02Z","timestamp":1789103282685,"version":"build-2803163510"},"reference-count":22,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,9,11]],"date-time":"2026-09-11T00:00:00Z","timestamp":1789084800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Cell. Infect. Microbiol."],"abstract":"<jats:p>\n                    Mpox virus (MPXV) is an enveloped double-stranded DNA virus belonging to the genus\n                    <jats:italic>Orthopoxvirus<\/jats:italic>\n                    within the family\n                    <jats:italic>Poxviridae<\/jats:italic>\n                    . It causes a zoonotic disease transmitted to humans through direct contact with infected animals or humans, as well as through exposure to contaminated materials. The aim of this study was to conduct a retrospective, comparative diagnostic evaluation of three commercial real-time PCR (qPCR) kits for the diagnosis of MPXV on an enriched, artificially balanced sample set that included 50% MPXV-positive samples. World Health Organization (WHO) reference molecular protocol was used as the reference comparator. A total of 300 clinical specimens, 150 of them MPXV-positive (265 skin lesion\/vesicle swabs and 35 serum samples), were used in this analysis. The Bioperfectus kit correctly identified 294\/300 samples, showing a sensitivity of 0.96 (95% CI: 0.93\u20130.99), a specificity of 1.00 (0.98\u20131.00), and a Cohen\u2019s Kappa coefficient of 0.96 (95% CI: 0.92\u20130.99), with an overall agreement of 0.98 (0.96\u20130.99). The VIASURE kit correctly detected 293\/300 specimens, yielding a sensitivity of 0.95 (0.92\u20130.99), specificity of 1.00 (0.98\u20131.00), Cohen\u2019s Kappa coefficient of 0.95 (95% CI: 0.92\u20130.99 and an overall agreement of 0.98 (0.95\u20130.99). The Singuway kit showed the highest concordance with the reference method, correctly classifying 295\/300 samples and presenting a sensitivity of 0.97 (0.94\u20131.00), specificity of 1.00 (0.98\u20131.00), Cohen\u2019s Kappa coefficient of 0.97 (95% CI: 0.94\u20131.00), with an overall agreement of 0.98 (0.97\u20131.00). This study demonstrates a good sensitivity and specificity, and strong overall agreement among the three commercial kits compared to the WHO protocol. Nevertheless, further analysis using unbiased clinical sample sets that reflect the true prevalence of MPXV-positive samples is needed for a real-world clinical performance evaluation.\n                  <\/jats:p>","DOI":"10.3389\/fcimb.2026.1831182","type":"journal-article","created":{"date-parts":[[2026,9,11]],"date-time":"2026-09-11T04:35:36Z","timestamp":1789101336000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["A retrospective comparative diagnostic evaluation of three commercial qPCR kits for the diagnosis of mpox virus on an enriched sample set"],"prefix":"10.3389","volume":"16","author":[{"given":"Darwin","family":"Paredes-N\u00fa\u00f1ez","sequence":"first","affiliation":[{"name":"One Health Research Group, Universidad de Las Am\u00e9ricas","place":["Quito, Ecuador"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Silvia","family":"Salgado","sequence":"additional","affiliation":[{"name":"Instituto Nacional de Salud P\u00fablica e Investigaci\u00f3n","place":["Guayaquil, Ecuador"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martha","family":"S\u00e1nchez","sequence":"additional","affiliation":[{"name":"Instituto Nacional de Salud P\u00fablica e Investigaci\u00f3n","place":["Guayaquil, Ecuador"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katherine Natalia","family":"Ch\u00e1vez-Toledo","sequence":"additional","affiliation":[{"name":"Universidad Cat\u00f3lica Santiago de Guayaquil","place":["Guayaquil, Ecuador"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Johanna","family":"Parrales","sequence":"additional","affiliation":[{"name":"One Health Research Group, Universidad de Las Am\u00e9ricas","place":["Quito, Ecuador"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andr\u00e9s","family":"Tinizaray","sequence":"additional","affiliation":[{"name":"One Health Research Group, Universidad de Las Am\u00e9ricas","place":["Quito, Ecuador"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jos\u00e9 Juli\u00e1n","family":"Z\u00fa\u00f1iga-Velarde","sequence":"additional","affiliation":[{"name":"Universidad Ecotec","place":["Samborond\u00f3n, Ecuador"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sol\u00f3n Alberto","family":"Orlando","sequence":"additional","affiliation":[{"name":"Instituto Nacional de Salud P\u00fablica e Investigaci\u00f3n","place":["Guayaquil, Ecuador"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miguel Angel","family":"Garcia-Bereguiain","sequence":"additional","affiliation":[{"name":"One Health Research Group, Universidad de Las Am\u00e9ricas","place":["Quito, Ecuador"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2026,9,11]]},"reference":[{"key":"B10","unstructured":"Laboratory testing for the monkeypox virus: interim guidance, 23 May 2022\n          \n          \n          2026"},{"key":"B2","doi-asserted-by":"publisher","first-page":"1175","DOI":"10.7326\/M22-1581","article-title":"A novel international monkeypox outbreak","volume":"175","author":"Adalja","year":"2022","journal-title":"Ann. 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Virol."},{"key":"B7","doi-asserted-by":"publisher","first-page":"679","DOI":"10.1056\/NEJMoa2207323","article-title":"Monkeypox virus infection in humans across 16 countries \u2014 April\u2013June 2022","volume":"387","author":"Thornhill","year":"2022","journal-title":"N. Engl. J. Med."},{"key":"B24","doi-asserted-by":"publisher","DOI":"10.26633\/RPSP.2024.131","article-title":"Diagnostic accuracy of polymerase chain reaction for detection of mpox in humans","author":"Unnikrishnan","year":"2026","journal-title":"Pan Am. J. Public Health"},{"key":"B9","unstructured":"WHO lists additional mpox diagnostic tests for emergency use\n          \n          \n          \n          2026"},{"key":"B16","doi-asserted-by":"publisher","first-page":"641","DOI":"10.1186\/s12916-025-04412-4","article-title":"A point-of-care testing device for monkeypox virus integrating recombinase polymerase amplification and lateral flow assay","volume":"23","author":"Yu","year":"2025","journal-title":"BMC Med."}],"container-title":["Frontiers in Cellular and Infection Microbiology"],"original-title":[],"link":[{"URL":"https:\/\/www.frontiersin.org\/articles\/10.3389\/fcimb.2026.1831182\/full","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,11]],"date-time":"2026-09-11T04:35:45Z","timestamp":1789101345000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.frontiersin.org\/articles\/10.3389\/fcimb.2026.1831182\/full"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9,11]]},"references-count":22,"alternative-id":["10.3389\/fcimb.2026.1831182"],"URL":"https:\/\/doi.org\/10.3389\/fcimb.2026.1831182","relation":{},"ISSN":["2235-2988"],"issn-type":[{"value":"2235-2988","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,9,11]]},"article-number":"1831182"}}