{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T08:07:43Z","timestamp":1782893263748,"version":"3.54.5"},"reference-count":28,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T00:00:00Z","timestamp":1782864000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004826","name":"Natural Science Foundation of Beijing Municipality","doi-asserted-by":"publisher","award":["7262008"],"award-info":[{"award-number":["7262008"]}],"id":[{"id":"10.13039\/501100004826","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Cell. Infect. Microbiol."],"abstract":"<jats:p>\n                    Respiratory syncytial virus (RSV) is a major cause of acute lower respiratory tract infections in children, highlighting the need for rapid and accessible molecular detection. Here, we developed two recombinase polymerase amplification (RPA)-based assays for the detection and differentiation of RSV-A and RSV-B, combining fluorescence readout or lateral flow biosensor (LFB) visualization, termed RSV-RPA-FLU and RSV-RPA-LFB, respectively. The workflow can be completed within 40\u00a0min, including sample preparation for 15\u00a0min, isothermal amplification for 20\u00a0min, and result interpretation within 5\u00a0min. Analytical sensitivity testing showed that the RSV-RPA-FLU assay detected both RSV-A and RSV-B at 10\n                    <jats:sup>4<\/jats:sup>\n                    copies\/\u03bcL. The RSV-RPA-LFB assay showed higher sensitivity, with detection limits of 6.05 \u00d7 10\u00b3 copies\/\u03bcL for RSV-A and 7.80 \u00d7 10\u00b2 copies\/\u03bcL for RSV-B. Specificity analysis against 11 non-RSV respiratory pathogens showed no cross-reactivity. Preliminary evaluation using simulated clinical samples further supported the feasibility of both assays in complex sample matrices. These results indicate that the RSV-RPA-FLU and RSV-RPA-LFB assays provide rapid, specific, and operationally simple analytical platforms for RSV-A\/RSV-B detection and differentiation, holding great potential for point-of-care RSV diagnosis and global prevention and control.\n                  <\/jats:p>","DOI":"10.3389\/fcimb.2026.1838582","type":"journal-article","created":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T06:12:15Z","timestamp":1782886335000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["Rapid, sensitive, and highly specific diagnosis of respiratory syncytial virus using recombinase polymerase amplification-based biosensor and fluorescence detection"],"prefix":"10.3389","volume":"16","author":[{"given":"Lei","family":"Yu","sequence":"first","affiliation":[{"name":"Capital Institute of Pediatrics, Chinese Academy of Medical Sciences & Peking Union Medical College","place":["Beijing, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bo","family":"Peng","sequence":"additional","affiliation":[{"name":"Capital Center for Children\u2019s Health, Capital Medical University, Capital Institute of Pediatrics","place":["Beijing, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qingjing","family":"Du","sequence":"additional","affiliation":[{"name":"Capital Center for Children\u2019s Health, Capital Medical University, Capital Institute of Pediatrics","place":["Beijing, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yangyang","family":"Wu","sequence":"additional","affiliation":[{"name":"Capital Center for Children\u2019s Health, Capital Medical University, Capital Institute of Pediatrics","place":["Beijing, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuang","family":"Liu","sequence":"additional","affiliation":[{"name":"Capital Center for Children\u2019s Health, Capital Medical University, Capital Institute of Pediatrics","place":["Beijing, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chunmei","family":"Zhu","sequence":"additional","affiliation":[{"name":"Capital Center for Children\u2019s Health, Capital Medical University, Capital Institute of Pediatrics","place":["Beijing, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chuanhe","family":"Liu","sequence":"additional","affiliation":[{"name":"Capital Center for Children\u2019s Health, Capital Medical University, Capital Institute of Pediatrics","place":["Beijing, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2026,7,1]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"3738","DOI":"10.1128\/jcm.01816-15","article-title":"Diagnostic accuracy of rapid antigen detection tests for respiratory syncytial virus infection: Systematic review and meta-analysis","volume":"53","author":"Chartrand","year":"2015","journal-title":"J. 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