{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,3]],"date-time":"2026-06-03T07:02:40Z","timestamp":1780470160878,"version":"3.54.1"},"reference-count":24,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,6,3]],"date-time":"2026-06-03T00:00:00Z","timestamp":1780444800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Cell. Infect. Microbiol."],"abstract":"<jats:p>Peste des petits ruminants (PPR) is an acute, contagious disease caused by the Peste des Petits Ruminants virus (PPRV), mainly affecting small ruminants such as goats and sheep. PPR poses a serious threat to the healthy development of the livestock industry and wildlife safety. To develop a rapid method for detecting antibodies against the PPRV N protein, we analyzed the dominant antigenic epitopes of the N protein of virulent and attenuated PPRV strains using bioinformatics. The dominant antigenic epitopes of the N gene were linked in tandem via overlapping PCR and cloned into a prokaryotic expression system, yielding the recombinant plasmid, pET28a-PPRV-2N (encoding amino acids 1\u2013250). Under optimized expression conditions, recombinant protein 2N was successfully produced after induction with isopropyl \u03b2-D-1-thiogalactopyranoside and purified. Using the purified recombinant PPRV-2N as the coating antigen in an enzyme-linked immunosorbent assay, the concentrations of the coating antigen and HRP-labeled monoclonal antibody against the PPRV N protein were optimized, resulting in the successful development of a blocking enzyme-linked immunosorbent assay (ELISA) for PPRV antibody detection. The new blocking ELISA detected positive serum at a minimum dilution of 1:10. The intra- and inter-assay coefficients of variation (CV) were both below 15%. No cross-reactivity was observed with sera positive for other important pathogens. When applied to 100 clinical serum samples, this method showed a 93% concordance with a commercial detection kit. The developed blocking ELISA method demonstrated good specificity, sensitivity, and repeatability, making it suitable for detecting PPRV antibodies. This technique supports the evaluation of PPRV vaccine-induced immune efficacy and aids in disease prevention and control.<\/jats:p>","DOI":"10.3389\/fcimb.2026.1841234","type":"journal-article","created":{"date-parts":[[2026,6,3]],"date-time":"2026-06-03T06:06:41Z","timestamp":1780466801000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["Establishment of a blocking enzyme-linked immunosorbent assay based on tandem expression of dominant antigenic epitopes of the nucleocapsid protein from attenuated and virulent peste des petits ruminants virus"],"prefix":"10.3389","volume":"16","author":[{"given":"Kaiyu","family":"Zhu","sequence":"first","affiliation":[{"name":"College of Agriculture and Animal Husbandry, Qinghai University","place":["Xining, Qinghai, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaolong","family":"Gao","sequence":"additional","affiliation":[{"name":"College of Agriculture and Animal Husbandry, Qinghai University","place":["Xining, Qinghai, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lina","family":"Tong","sequence":"additional","affiliation":[{"name":"College of Agriculture and Animal Husbandry, Qinghai University","place":["Xining, Qinghai, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinchao","family":"Du","sequence":"additional","affiliation":[{"name":"College of Agriculture and Animal Husbandry, Qinghai University","place":["Xining, Qinghai, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yun","family":"Chen","sequence":"additional","affiliation":[{"name":"College of Agriculture and Animal Husbandry, Qinghai University","place":["Xining, Qinghai, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jinxin","family":"Xie","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Xinjiang Agricultural University","place":["Urumqi, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shanhui","family":"Ren","sequence":"additional","affiliation":[{"name":"State Key Laboratory for Animal Disease Control and Prevention, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences","place":["Lanzhou, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2026,6,3]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"1831","DOI":"10.1099\/0022-1317-83-8-1831","article-title":"Significant differences in nucleocapsid morphology within the Paramyxoviridae","volume":"83","author":"Bhella","year":"2002","journal-title":"J. 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