{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,7]],"date-time":"2026-09-07T06:08:23Z","timestamp":1788761303701,"version":"build-2803163510"},"reference-count":52,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,9,7]],"date-time":"2026-09-07T00:00:00Z","timestamp":1788739200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Cell. Infect. Microbiol."],"abstract":"<jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Sepsis-associated encephalopathy (SAE) is a common complication of sepsis. Its underlying mechanisms remain incompletely understood, and effective treatments are still lacking. Recent studies have shown that the microbiota-gut-brain axis may play a key role in the pathogenesis of SAE, but its potential mechanisms have not yet been clarified.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>In this study, we used a lipopolysaccharide (LPS)-induced zebrafish endotoxemia model and systematically characterized microbiota\u2013gut\u2013brain axis-associated alterations using multi-omics profiling combined with histopathology, behavioral assessment, and blood\u2013brain barrier integrity assays.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Our results showed that LPS exposure induced intestinal inflammation and barrier disruption, neurovascular dysfunction, anxiety-like behavior, and impaired cognitive function in zebrafish. Gut microbiota profiling revealed marked compositional alterations, accompanied by widespread metabolic disturbances in intestinal and brain tissues. Brain transcriptomic analysis showed that the differentially expressed genes were closely associated with the PI3K-Akt signaling pathway, cell adhesion, and autophagy-related pathways. Cross-omics association analyses suggested potential associations among gut microbial dysbiosis, metabolic disturbances, and brain molecular responses.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>These findings suggest that disruption of the microbiota\u2013gut\u2013brain axis may contribute to LPS-induced SAE-like neurobehavioral abnormalities and provide a basis for further mechanistic and therapeutic studies.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.3389\/fcimb.2026.1849929","type":"journal-article","created":{"date-parts":[[2026,9,7]],"date-time":"2026-09-07T05:35:10Z","timestamp":1788759310000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["Multi-omics profiling reveals microbiota\u2013gut\u2013brain axis dysregulation in an LPS-induced model of sepsis-associated encephalopathy"],"prefix":"10.3389","volume":"16","author":[{"given":"Qi","family":"Shan","sequence":"first","affiliation":[{"name":"Department of Intensive Care Unit (Internal Medicine), The First Affiliated Hospital, and College of Clinical Medicine of Henan University of Science and Technology","place":["Luoyang, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ying","family":"Yu","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, First Affiliated Hospital of Chongqing Medical University","place":["Chongqing, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yongjie","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Anesthesiology, The First Affiliated Hospital, and College of Clinical Medicine of Henan University of Science and Technology","place":["Luoyang, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yiming","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory, The First Affiliated Hospital, College of Clinical Medicine of Henan University of Science and Technology","place":["Luoyang, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianqiang","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Intensive Care Unit (Internal Medicine), The First Affiliated Hospital, and College of Clinical Medicine of Henan University of Science and Technology","place":["Luoyang, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Intensive Care Unit (Internal Medicine), The First Affiliated Hospital, and College of Clinical Medicine of Henan University of Science and Technology","place":["Luoyang, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qiang","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Rehabilitation, The Third People\u2019s Provincial Hospital of Henan Province","place":["Zhengzhou, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hong","family":"Fu","sequence":"additional","affiliation":[{"name":"Department of Rehabilitation, The Third People\u2019s Provincial Hospital of Henan Province","place":["Zhengzhou, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhifeng","family":"Qu","sequence":"additional","affiliation":[{"name":"Department of Medical Equipment, The First Affiliated Hospital, and College of Clinical Medicine of Henan University of Science and Technology","place":["Luoyang, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiajia","family":"Duan","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory, The First Affiliated Hospital, College of Clinical Medicine of Henan University of Science and Technology","place":["Luoyang, China"]},{"name":"Luoyang Key Laboratory of Zebrafish Toxicology Research, Luoyang Key Laboratory of Transplantation and Immunological Studies for Haematological Diseases, and College of Clinical Medicine of Henan University of Science and Technology","place":["Luoyang, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qizhi","family":"Fu","sequence":"additional","affiliation":[{"name":"Department of Intensive Care Unit (Internal Medicine), The First Affiliated Hospital, and College of Clinical Medicine of Henan University of Science and Technology","place":["Luoyang, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2026,9,7]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2023.1270123","article-title":"Role of Legionella pneumophila outer membrane vesicles in host-pathogen interaction","volume":"14","author":"Ayesha","year":"2023","journal-title":"Front. 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