{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,7]],"date-time":"2026-07-07T05:14:32Z","timestamp":1783401272745,"version":"3.54.6"},"reference-count":96,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,7,7]],"date-time":"2026-07-07T00:00:00Z","timestamp":1783382400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Cell. Infect. Microbiol."],"abstract":"<jats:sec>\n                    <jats:title>Introduction<\/jats:title>\n                    <jats:p>The gut microbiome is increasingly recognized as a central factor in carcinogenesis. Dietary components and therapeutic interventions, including probiotics, may influence microbial composition and function, thereby modulating cancer risk. Del\u2212Immune V, a metabiotic supplement derived from Lactobacillus rhamnosus, has demonstrated immunomodulatory properties. This study investigates its role in microbiome restructuring and patient\u2212reported outcomes in colorectal cancer patients during the perioperative period.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Materials and Methods<\/jats:title>\n                    <jats:p>A randomized, controlled, double\u2212blind Phase I trial was conducted in 39 colorectal cancer patients undergoing elective resection, assigned to Del\u2212Immune V (n=22) or placebo (n=17). Participants received two capsules daily (100 mg each), starting 7\u201315 days before surgery and continuing until 15 days postoperatively. Blood and fecal samples were collected at baseline and day 60 to assess IL\u22126, CRP, CEA, and microbiome composition. Patient\u2212reported outcomes were measured using the EORTC QLQ\u2212C30 questionnaire. Microbiome profiling was performed using 16S rRNA gene sequencing with PICRUSt-based functional inference.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Del\u2212Immune V significantly reduced IL\u22126 (p=0.012) and supported CRP decline, while quality\u2212of\u2212life scores improved across multiple domains. Microbiome analyses revealed enrichment of short\u2212chain fatty acid\u2013producing genera (\n                      <jats:italic>Bifidobacterium, Agathobacter, Gemmiger, Phocaeicola<\/jats:italic>\n                      ) and decline of CRC\u2212associated taxa (\n                      <jats:italic>Fusobacterium<\/jats:italic>\n                      ), with a significant improvement in the dysbiosis index (p=0.024).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Del\u2212Immune V demonstrated immunomodulatory activity, evidenced by reductions in IL\u22126 and CRP, alongside improvements in patient\u2212reported quality of life. These effects were accompanied by restructuring of the gut microbiome, characterized by enrichment of protective commensals and reduction of CRC\u2212associated taxa. Collectively, findings support Del\u2212Immune V as a safe adjunctive therapy in colorectal cancer surgery, with potential to enhance recovery and long\u2212term outcomes.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.3389\/fcimb.2026.1853373","type":"journal-article","created":{"date-parts":[[2026,7,7]],"date-time":"2026-07-07T04:21:51Z","timestamp":1783398111000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["Del immune V and microbiome restructuring in colorectal cancer surgery: a randomized double blind placebo controlled trial"],"prefix":"10.3389","volume":"16","author":[{"given":"Gissel","family":"Garc\u00eda","sequence":"first","affiliation":[{"name":"Gissel Garc\u00eda, Pathology Department, Hermanos Ameijeiras Clinical and Surgical Hospital","place":["La Habana, Cuba"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Antonio","family":"D\u00edaz","sequence":"additional","affiliation":[{"name":"Antonio D\u00edaz.Statistical and Research Department, Hermanos Ameijeiras Clinical and Surgical Hospital","place":["La Habana, Cuba"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"LLipsy T.","family":"Fern\u00e1ndez","sequence":"additional","affiliation":[{"name":"Lllipsy T Fern\u00e1ndez Surgery Department, Hermanos Ameijeiras Clinical and Surgical Hospital","place":["La Habana, Cuba"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mirka","family":"Bernal","sequence":"additional","affiliation":[{"name":"Mirka Bernal Clinical Laboratory Department, Hermanos Ameijeiras Clinical and Surgical Hospital","place":["La Habana, Cuba"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Josanne","family":"Soto","sequence":"additional","affiliation":[{"name":"Josanne Soto Clinical Laboratory Department, Hermanos Ameijeiras Clinical and Surgical Hospital","place":["La Habana, Cuba"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gilda","family":"N\u00fa\u00f1ez","sequence":"additional","affiliation":[{"name":"Gilda N\u00fa\u00f1ez Surgery Department, Hermanos Ameijeiras Clinical and Surgical Hospital","place":["La Habana, Cuba"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vilma","family":"Fleites","sequence":"additional","affiliation":[{"name":"Vilma Fleites: Oncology Department, Hermanos Ameijeiras Clinical and Surgical Hospital","place":["La Habana, Cuba"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ernesto","family":"Arteaga","sequence":"additional","affiliation":[{"name":"Ernesto Arteaga Pathology Department, Hermanos Ameijeiras Clinical and Surgical Hospital","place":["La Habana, Cuba"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liubov","family":"Sichel","sequence":"additional","affiliation":[{"name":"Liubov Sichel: Stellar Biotics, LLC.","place":["Rockleigh, NJ, United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ra\u00fal de Jes\u00fas","family":"Cano","sequence":"additional","affiliation":[{"name":"Ra\u00fal de Jes\u00fas Cano, Biological Sciences Department, California Polytechnic State University","place":["San Luis Obispo, CA, United States"]},{"name":"8 Academia de Ciencias de Cuba","place":["La Habana, Cuba"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2026,7,7]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2026.1761985","article-title":"Overview of the immunomodulatory role of bacterial probiotic-derived peptidoglycan: from molecular insights to therapeutic application","volume":"17","author":"Allela","year":"2026","journal-title":"Front. 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