{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,29]],"date-time":"2026-06-29T05:47:51Z","timestamp":1782712071615,"version":"3.54.5"},"reference-count":63,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,6,29]],"date-time":"2026-06-29T00:00:00Z","timestamp":1782691200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Cell. Infect. Microbiol."],"abstract":"<jats:p>\n                    Infective endocarditis is a rare but severe disease in humans and dogs which can be caused by\n                    <jats:italic>Streptococcus canis<\/jats:italic>\n                    . To understand how\n                    <jats:italic>S. canis<\/jats:italic>\n                    can adhere to and invade the endocardium, we combined a high-throughput approach called transposon directed insertion-site sequencing (TraDIS) with a physiologically relevant endothelial cell infection model that incorporates venous-range shear stress to identify genes encoding factors relevant for bacterial adherence and invasion to vascular human cells under simulation of physiological blood flow conditions. A saturated transposon library of clinical strain IMT49926 was screened in a microfluidic infection assay, enabling genome-wide selection of mutants impaired in endothelial adhesion. Comparative analysis of input and non-adherent output pools revealed several candidate genes, including a fibronectin-binding LPXTG-anchored surface protein with high homology to streptococcal serum opacity factors (SOFs). Our findings identified ScSOF as a multifunctional surface protein that plays an important role in the infection potential of\n                    <jats:italic>S. canis<\/jats:italic>\n                    . It facilitates adhesion to endothelial cells, prevents endothelial wound closure, contributes to the streptococcal surface architecture, binds fibronectin, opacifies serum, and inhibits \u03b2-hemolytic activity. These properties make ScSOF a compelling candidate for further, more targeted studies to confirm it as a virulence factor in\n                    <jats:italic>S. canis<\/jats:italic>\n                    that can be used for vaccine development or therapeutic targeting.\n                  <\/jats:p>","DOI":"10.3389\/fcimb.2026.1867913","type":"journal-article","created":{"date-parts":[[2026,6,29]],"date-time":"2026-06-29T05:39:27Z","timestamp":1782711567000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["Discovery of a new fibronectin-binding surface protein of Streptococcus canis with serum opacification activity through transposon directed insertion-site sequencing"],"prefix":"10.3389","volume":"16","author":[{"given":"Miriam","family":"Katsburg","sequence":"first","affiliation":[{"name":"Institute of Microbiology and Epizootics, Centre for Infection Medicine, Freie Universit\u00e4t Berlin","place":["Berlin, Germany"]},{"name":"Veterinary Centre for Resistance Research (TZR), Freie Universit\u00e4t Berlin","place":["Berlin, Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna","family":"Kopenhagen","sequence":"additional","affiliation":[{"name":"Institute of Microbiology, Technische Universit\u00e4t Braunschweig","place":["Braunschweig, Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Etienne","family":"Aubry","sequence":"additional","affiliation":[{"name":"Institute of Microbiology and Epizootics, Centre for Infection Medicine, Freie Universit\u00e4t Berlin","place":["Berlin, Germany"]},{"name":"Veterinary Centre for Resistance Research (TZR), Freie Universit\u00e4t Berlin","place":["Berlin, Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mathias","family":"M\u00fcsken","sequence":"additional","affiliation":[{"name":"Central Facility for Microscopy, Helmholtz Centre for Infection Research","place":["Braunschweig, Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gloria","family":"Riebesell","sequence":"additional","affiliation":[{"name":"Institute of Microbiology, Technische Universit\u00e4t Braunschweig","place":["Braunschweig, Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Deborah","family":"Simmert","sequence":"additional","affiliation":[{"name":"Institute of Microbiology, Technische Universit\u00e4t Braunschweig","place":["Braunschweig, Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sanja","family":"Haake","sequence":"additional","affiliation":[{"name":"Institute of Microbiology, Technische Universit\u00e4t Braunschweig","place":["Braunschweig, Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Inga","family":"Eichhorn","sequence":"additional","affiliation":[{"name":"Genome Competence Centre (MF1), Robert Koch Institute (RKI)","place":["Berlin, Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Silver A.","family":"Wolf","sequence":"additional","affiliation":[{"name":"Genome Competence Centre (MF1), Robert Koch Institute (RKI)","place":["Berlin, Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Simone","family":"Bergmann","sequence":"additional","affiliation":[{"name":"Institute of Microbiology, Technische Universit\u00e4t Braunschweig","place":["Braunschweig, Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marcus","family":"Fulde","sequence":"additional","affiliation":[{"name":"Institute of Microbiology and Epizootics, Centre for Infection Medicine, Freie Universit\u00e4t Berlin","place":["Berlin, Germany"]},{"name":"Veterinary Centre for Resistance Research (TZR), Freie Universit\u00e4t Berlin","place":["Berlin, Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2026,6,29]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"1462.e1","DOI":"10.1016\/j.cjca.2014.07.013","article-title":"First reported human case of native mitral infective endocarditis caused by Streptococcus canis","volume":"30","author":"Amsallem","year":"2014","journal-title":"Can. 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