{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,14]],"date-time":"2026-08-14T11:27:31Z","timestamp":1786706851072,"version":"build-2736575974"},"reference-count":52,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,8,14]],"date-time":"2026-08-14T00:00:00Z","timestamp":1786665600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004167","name":"Medicines for Malaria Venture","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004167","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000865","name":"Bill and Melinda Gates Foundation","doi-asserted-by":"publisher","award":["OPP1141292"],"award-info":[{"award-number":["OPP1141292"]}],"id":[{"id":"10.13039\/100000865","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Cell. Infect. Microbiol."],"abstract":"<jats:sec>\n                    <jats:title>Introduction<\/jats:title>\n                    <jats:p>\n                      <jats:italic>Plasmodium cynomolgi<\/jats:italic>\n                      is one of the few malaria parasite species that forms dormant liver stages known as hypnozoites and is therefore a suitable model for\n                      <jats:italic>Plasmodium vivax<\/jats:italic>\n                      , the causative agent of recurrent malaria. Very little is known about the biology and pharmacology of liver stage dormancy, which hampers the search for compounds with anti-hypnozoite activity.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      We present the development of a\n                      <jats:italic>P. cynomolgi in vitro<\/jats:italic>\n                      infection model using stem cell-derived hepatocyte-like cells from\n                      <jats:italic>Macaca fascicularis.<\/jats:italic>\n                      iPS cells were established on feeder free condition and differentiated into hepatocyte-like cells via inducible overexpression of key transcription factors.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      The generated cells were infected with\n                      <jats:italic>P. cynomolgi<\/jats:italic>\n                      sporozoites, and hypnozoite formation as well as schizont development were confirmed by immunofluorescence microscopy.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Discussion<\/jats:title>\n                    <jats:p>\n                      This simian iPS-derived model is a promising alternative to current\n                      <jats:italic>P. vivax in vitro<\/jats:italic>\n                      infection systems to study the mechanisms underlying liver stage dormancy.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.3389\/fcimb.2026.1871289","type":"journal-article","created":{"date-parts":[[2026,8,14]],"date-time":"2026-08-14T10:30:36Z","timestamp":1786703436000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["Simian iPS-derived hepatocytes support Plasmodium cynomolgi infection and hypnozoite formation"],"prefix":"10.3389","volume":"16","author":[{"given":"M\u00e9lanie","family":"Pellisson","sequence":"first","affiliation":[{"name":"Swiss Tropical and Public Health Institute (Swiss TPH)","place":["Allschwil, Switzerland"]},{"name":"University of Basel","place":["Basel, Switzerland"]},{"name":"Novartis Institutes for Biomedical Research, Novartis Pharma AG","place":["Basel, Switzerland"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anne-Marie","family":"Zeeman","sequence":"additional","affiliation":[{"name":"Department of Parasitology, Biomedical Primate Research Centre","place":["Rijswijk, Netherlands"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thierry","family":"Doll","sequence":"additional","affiliation":[{"name":"Novartis Institutes for Biomedical Research, Novartis Pharma AG","place":["Basel, Switzerland"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lucy","family":"Kirchhofer-Allan","sequence":"additional","affiliation":[{"name":"Novartis Institute for Tropical Diseases, Novartis Pharma AG","place":["Emeryville, CA, United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sven","family":"Schuierer","sequence":"additional","affiliation":[{"name":"Novartis Institutes for Biomedical Research, Novartis Pharma AG","place":["Basel, Switzerland"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guglielmo","family":"Roma","sequence":"additional","affiliation":[{"name":"Novartis Institutes for Biomedical Research, Novartis Pharma AG","place":["Basel, Switzerland"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Erika L.","family":"Flannery","sequence":"additional","affiliation":[{"name":"Novartis Institute for Tropical Diseases, Novartis Pharma AG","place":["Emeryville, CA, United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sebastian","family":"Mikolajczak","sequence":"additional","affiliation":[{"name":"Novartis Institute for Tropical Diseases, Novartis Pharma AG","place":["Emeryville, CA, United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Clemens H. 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