{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T04:59:05Z","timestamp":1790657945671,"version":"4.1.0"},"reference-count":43,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T00:00:00Z","timestamp":1790640000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100005227","name":"Jilin Provincial Department of Health","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100005227","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Cell. Infect. Microbiol."],"abstract":"<jats:p>\n                    Millions of fungal infections are reported every year, while the current antifungal drugs are so few that the clinical choices are limited. The opportunistic fungal pathogen\n                    <jats:italic>Candida glabrata<\/jats:italic>\n                    (reclassified as\n                    <jats:italic>Nakaseomyces glabratus<\/jats:italic>\n                    ) caused more infections than any other\n                    <jats:italic>Candida<\/jats:italic>\n                    species except\n                    <jats:italic>C. albicans<\/jats:italic>\n                    . This research sought to explore the antifungal effects of isobavachalcone (IBC, a bioactive chalcone from\n                    <jats:italic>Psoralea corylifolia<\/jats:italic>\n                    ) on\n                    <jats:italic>C. glabrata<\/jats:italic>\n                    planktonic growth and biofilms. Our results showed that IBC has potent antifungal activity against\n                    <jats:italic>C. glabrata<\/jats:italic>\n                    (MIC: 4-5 \u03bcg\/mL), including both the standard strain and clinical isolates. This compound could also inhibit\n                    <jats:italic>C. glabrata<\/jats:italic>\n                    biofilm formation and development. Its antifungal mechanism involves intracellular ROS overproduction and cell membrane impairments, which were revealed by confocal laser scanning microscope observation after fluorescent staining of ROS and plasma membrane.\n                    <jats:italic>N<\/jats:italic>\n                    -acetyl-cysteine rescue tests also confirmed that the ROS-inducing activity of IBC contributed to its antifungal and antibiofilm effects on\n                    <jats:italic>C. glabrata<\/jats:italic>\n                    . Micrographs obtained with electron microscope revealed as well the impairments to\n                    <jats:italic>C. glabrata<\/jats:italic>\n                    plasma membrane caused by IBC. In conclusion, our results obtained here indicated that IBC is a promising antifungal candidate for treating\n                    <jats:italic>C. glabrata<\/jats:italic>\n                    infections.\n                  <\/jats:p>","DOI":"10.3389\/fcimb.2026.1874660","type":"journal-article","created":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T04:41:05Z","timestamp":1790656865000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["The antifungal and antibiofilm effects of isobavachalcone on Candida glabrata (Nakaseomyces glabratus) are associated with intracellular ROS production and cell membrane damages"],"prefix":"10.3389","volume":"16","author":[{"given":"Longfei","family":"Yang","sequence":"first","affiliation":[{"name":"Jilin Provincial Key Laboratory on Molecular and Chemical Genetics, The Second Hospital of Jilin University","place":["Changchun, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanfeng","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Blood Transfusion, The Second Hospital of Jilin University","place":["Changchun, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory, The Second Hospital of Jilin University","place":["Changchun, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lufei","family":"Wang","sequence":"additional","affiliation":[{"name":"Eye Center, The Second Hospital of Jilin University","place":["Changchun, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xin","family":"Liu","sequence":"additional","affiliation":[{"name":"Eye Center, The Second Hospital of Jilin University","place":["Changchun, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2026,9,29]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"843","DOI":"10.1007\/s10534-025-00680-y","article-title":"Investigation of antifungal and antibiofilm activities of green synthesized silver nanoparticles against Candida glabrata","volume":"38","author":"Ahamad","year":"2025","journal-title":"BioMetals"},{"key":"B2","doi-asserted-by":"publisher","first-page":"428","DOI":"10.1016\/j.jinf.2023.08.001","article-title":"European candidaemia is characterised by notable differential epidemiology and susceptibility pattern: Results from the ECMM Candida III study","volume":"87","author":"Arendrup","year":"2023","journal-title":"J. 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