{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,21]],"date-time":"2026-08-21T05:23:18Z","timestamp":1787289798880,"version":"build-2736575974"},"reference-count":41,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,8,21]],"date-time":"2026-08-21T00:00:00Z","timestamp":1787270400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Cell. Infect. Microbiol."],"abstract":"\n Introduction<\/jats:title>\n \n Candida tropicalis<\/jats:italic>\n is responsible for a substantial proportion of invasive candidiasis cases and is recognized as the second most prevalent causative agent, only behind\n Candida albicans<\/jats:italic>\n . A documented rise in invasive systemic infections and sepsis due to\n C. tropicalis<\/jats:italic>\n has contributed to the increased mortality among vulnerable patient populations, highlighting the critical need for heightened clinical vigilance. Early detection of\n C. tropicalis<\/jats:italic>\n is essential for guiding appropriate antifungal therapy and is associated with improved clinical outcomes.\n <\/jats:p>\n <\/jats:sec>\n \n Methods<\/jats:title>\n \n To address the need for rapid and reliable detection of\n C. tropicalis<\/jats:italic>\n , this research aimed to develop a novel closed dumbbell-mediated isothermal amplification (CDA) assay characterized by high speed, specificity, and efficiency. A set of specific CDA primers targeting the conserved\n ITS2<\/jats:italic>\n gene of\n C. tropicalis<\/jats:italic>\n was successfully designed, selected, and validated, resulting in a simple and efficient detection method. The\n C. tropicalis<\/jats:italic>\n CDA assay for\n in vitro<\/jats:italic>\n diagnostic applications demonstrated robust performance, with its sensitivity, robustness, stability, and specificity systematically evaluated using 364 batches of diverse DNA samples.\n <\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n \n The\n C. tropicalis<\/jats:italic>\n CDA assay demonstrated perfect diagnostic performance with apparent 100% sensitivity (95%CI = 97.0%\u2013100%), specificity (95%CI = 98.5%\u2013100%), and accuracy (95%CI = 99.0%\u2013100%) in a primary evaluation using 364 batches of diverse DNA samples, with a limit of detection of 5.6 \u00d7 10\n \u22126<\/jats:sup>\n ng\/\u03bcl (approximately 10 copies\/\u03bcl), which is not significantly different from that of real-time quantitative PCR (qPCR), indicating comparable analytical sensitivity.\n <\/jats:p>\n <\/jats:sec>\n \n Discussion<\/jats:title>\n \n Validated in both real-time fluorescence and endpoint visual formats, the\n C. tropicalis<\/jats:italic>\n CDA assay demonstrated high specificity, sensitivity, and accuracy, along with robust stability and reproducibility, supporting its future application potential.\n <\/jats:p>\n <\/jats:sec>","DOI":"10.3389\/fcimb.2026.1875246","type":"journal-article","created":{"date-parts":[[2026,8,21]],"date-time":"2026-08-21T04:23:03Z","timestamp":1787286183000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["Sensitive and rapid detection of Candida tropicalis using closed dumbbell-mediated isothermal amplification"],"prefix":"10.3389","volume":"16","author":[{"given":"Yangbo","family":"Fu","sequence":"first","affiliation":[{"name":"The First Affiliated Hospital of Ningbo University","place":["Ningbo, Zhejiang, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jing","family":"Shen","sequence":"additional","affiliation":[{"name":"The First Affiliated Hospital of Ningbo University","place":["Ningbo, Zhejiang, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haizhong","family":"Jiang","sequence":"additional","affiliation":[{"name":"The First Affiliated Hospital of Ningbo University","place":["Ningbo, Zhejiang, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanli","family":"Zhang","sequence":"additional","affiliation":[{"name":"The First Affiliated Hospital of Ningbo University","place":["Ningbo, Zhejiang, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xuhan","family":"Chen","sequence":"additional","affiliation":[{"name":"Ningbo No. 2 Hospital, Wenzhou Medical University","place":["Ningbo, Zhejiang, China"]},{"name":"Ningbo Institute of Life and Health Industry, University of Chinese Academy of Sciences","place":["Ningbo, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rui","family":"Mao","sequence":"additional","affiliation":[{"name":"Ningbo No. 2 Hospital, Wenzhou Medical University","place":["Ningbo, Zhejiang, China"]},{"name":"Ningbo Institute of Life and Health Industry, University of Chinese Academy of Sciences","place":["Ningbo, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2026,8,21]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"77","DOI":"10.1128\/jcm.44.1.77-84.2006","article-title":"Internal transcribed spacer sequencing versus biochemical profiling for identification of medically important yeasts","volume":"44","author":"Ciardo","year":"2006","journal-title":"J. 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