{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,31]],"date-time":"2026-07-31T04:59:18Z","timestamp":1785473958873,"version":"3.56.0"},"reference-count":39,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,7,31]],"date-time":"2026-07-31T00:00:00Z","timestamp":1785456000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Cell. Infect. Microbiol."],"abstract":"<jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>\n                      Carbapenem-resistant\n                      <jats:italic>Acinetobacter baumannii<\/jats:italic>\n                      (CRAB) is priority pathogen for nosocomial infection control worldwide. This study aimed to elucidate differences in distribution, genotyping and antimicrobial resistance patterns between CRAB and carbapenem-susceptible\n                      <jats:italic>Acinetobacter baumannii<\/jats:italic>\n                      (CSAB) strains isolated from healthcare environments.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Materials and methods<\/jats:title>\n                    <jats:p>\n                      A total of 1,812 environmental samples were collected from 10 medical institutions in Pudong New Area between June and December 2024 for the identification of\n                      <jats:italic>A. baumannii<\/jats:italic>\n                      . Antimicrobial susceptibility testing, whole-genome sequencing, and bioinformatic analysis were performed to reveal molecular characteristics of\n                      <jats:italic>A. baumannii.<\/jats:italic>\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      A total of 81\u00a0\n                      <jats:italic>A. baumannii<\/jats:italic>\n                      strains were detected, with 39.51% CRAB (\n                      <jats:italic>n<\/jats:italic>\n                      \u00a0=\u00a032) and 60.49% CSAB (\n                      <jats:italic>n<\/jats:italic>\n                      \u00a0=\u00a049). CRAB strains were highly prevalent in intensive care units (ICUs, accounting for 83.33%) and patient-contact items, whereas CSAB were more evenly distributed. MLST analysis identified ST2 as dominant CRAB clone (78.13%), followed by ST164 (18.75%). CRAB isolates demonstrated 100% resistance to multiple antibiotics such as piperacillin\/tazobactam and ciprofloxacin, whereas all CSAB isolates were susceptible to tested antibiotics except one ampicillin\/sulbactam-resistant isolate. Carbapenem resistance in CRAB was predominantly mediated by class D carbapenemases, with carriage rate of the\n                      <jats:italic>bla<\/jats:italic>\n                      <jats:sub>OXA-23<\/jats:sub>\n                      gene reaching 96.88%. Notably, six ST164 isolates co-harbored class B and class D carbapenemase genes, which may elevate resistance severity. Bioinformatic analysis indicated that 22.20% of resistance plasmids in CRAB were predicted to be conjugative. CRAB exhibited a more diverse and extensive virulence gene profile than CSAB strains.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>The ICU environment was critical for CRAB control. ST2 and ST164 represented clones worthy of attention. CRAB exhibited stronger resistance, harbored more putatively conjugative plasmids and richer virulence-associated genes than CSAB, highlighting the need for targeted disinfection and surveillance of prevalent clones.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.3389\/fcimb.2026.1878371","type":"journal-article","created":{"date-parts":[[2026,7,31]],"date-time":"2026-07-31T04:24:54Z","timestamp":1785471894000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["Comparative genomic insights into carbapenem-resistant versus susceptible Acinetobacter baumannii in Shanghai hospital settings: a multi-center molecular surveillance study"],"prefix":"10.3389","volume":"16","author":[{"given":"Yue","family":"Zhang","sequence":"first","affiliation":[{"name":"Shanghai Pudong New Area Center for Disease Control and Prevention (Shanghai Pudong New Area Health Supervision Institute)","place":["Shanghai, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanru","family":"Liang","sequence":"additional","affiliation":[{"name":"Shanghai Pudong New Area Center for Disease Control and Prevention (Shanghai Pudong New Area Health Supervision Institute)","place":["Shanghai, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuanping","family":"Wang","sequence":"additional","affiliation":[{"name":"Shanghai Pudong New Area Center for Disease Control and Prevention (Shanghai Pudong New Area Health Supervision Institute)","place":["Shanghai, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yiying","family":"Xu","sequence":"additional","affiliation":[{"name":"Shanghai Pudong New Area Center for Disease Control and Prevention (Shanghai Pudong New Area Health Supervision Institute)","place":["Shanghai, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ye","family":"Qiu","sequence":"additional","affiliation":[{"name":"Shanghai Pudong New Area Center for Disease Control and Prevention (Shanghai Pudong New Area Health Supervision Institute)","place":["Shanghai, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bing","family":"Zhao","sequence":"additional","affiliation":[{"name":"Shanghai Pudong New Area Center for Disease Control and Prevention (Shanghai Pudong New Area Health Supervision Institute)","place":["Shanghai, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yifeng","family":"Shen","sequence":"additional","affiliation":[{"name":"Shanghai Pudong New Area Center for Disease Control and Prevention (Shanghai Pudong New Area Health Supervision Institute)","place":["Shanghai, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiao","family":"Wang","sequence":"additional","affiliation":[{"name":"Shanghai Pudong New Area Center for Disease Control and Prevention (Shanghai Pudong New Area Health Supervision Institute)","place":["Shanghai, China"]},{"name":"Institute of Respiratory Medicine, Chinese Academy of Medical Sciences, Peking Union Medical College","place":["Beijing, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2026,7,31]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0317798","article-title":"Predominance of extensively-drug resistant Acinetobacter baumannii carrying bla OXA-23 in Jordanian patients admitted to the intensive care units","volume":"20","author":"Ababneh","year":"2025","journal-title":"PloS One"},{"key":"B2","doi-asserted-by":"publisher","DOI":"10.1111\/ijcp.14998","article-title":"Molecular epidemiology of carbapenem-resistant Acinetobacter baumannii isolated from three major hospitals in Jordan","volume":"75","author":"Ababneh","year":"2021","journal-title":"Int. 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