{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T05:00:03Z","timestamp":1790312403773,"version":"4.1.0"},"reference-count":69,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T00:00:00Z","timestamp":1790294400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Cell. Infect. Microbiol."],"abstract":"<jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>\n                      The earliest interactions between\n                      <jats:italic>Fasciola hepatica<\/jats:italic>\n                      newly excysted juveniles (FhNEJ) and the intestinal epithelium remain poorly understood, partly due to the limited availability of physiologically relevant\n                      <jats:italic>in vitro<\/jats:italic>\n                      models. Here, we establish a human duodenum organoid-derived epithelial monolayer (ODM) co-culture system to investigate the early host\u2013parasite interface, providing a controlled experimental platform for analysing interactions between FhNEJ and the intestinal epithelium.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>ODMs representing the human duodenum were co-cultured with either live FhNEJ or a tegument-enriched protein fraction derived from the parasites. The ODM co-culture system was subsequently employed to assess FhNEJ viability and parasite\u2013epithelial interactions together with complementary functional and molecular read-outs. These included measurements of trans-epithelial electrical resistance, fluorescein permeability assays, immunofluorescence analysis of intercellular epithelial junctions, and quantitative proteomic profiling.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>The ODM\u2013FhNEJ co-culture system supported sustained parasite viability and enabled direct visualisation of intimate parasite\u2013epithelial interactions. Functional and structural analyses revealed epithelial barrier dysfunction upon exposure to FhNEJ, whilst quantitative proteomics generated an exploratory molecular dataset identifying candidate proteins associated with epithelial junction organisation, immune signalling, cellular stress, and mechanotransduction.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>\n                      Together, these findings establish human intestinal ODMs as a physiologically relevant experimental platform for investigating the earliest stages of\n                      <jats:italic>F. hepatica<\/jats:italic>\n                      infection. By integrating functional, imaging-based and quantitative proteomic analyses within a single experimental framework, this model provides a valuable resource for generating and testing hypotheses regarding host\u2013parasite interactions during early-stage fasciolosis.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.3389\/fcimb.2026.1886545","type":"journal-article","created":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T04:27:20Z","timestamp":1790310440000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["Development of a novel organoid-based two-dimensional system to model the intestinal host\u2013parasite interface in fasciolosis"],"prefix":"10.3389","volume":"16","author":[{"given":"Judit","family":"Serrat","sequence":"first","affiliation":[{"name":"Laboratory of Helminth Parasites of Zoonotic Importance (ATENEA), Institute of Natural Resources and Agrobiology of Salamanca (IRNASA-CSIC)","place":["Salamanca, Spain"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mar","family":"Siles-Lucas","sequence":"additional","affiliation":[{"name":"Laboratory of Helminth Parasites of Zoonotic Importance (ATENEA), Institute of Natural Resources and Agrobiology of Salamanca (IRNASA-CSIC)","place":["Salamanca, Spain"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Toni","family":"Aebischer","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases, Unit for Mycotic and Parasitic Agents and Mycobacteria, Robert Koch Institute","place":["Berlin, Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christian","family":"Klotz","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases, Unit for Mycotic and Parasitic Agents and Mycobacteria, Robert Koch Institute","place":["Berlin, Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Javier","family":"Gonz\u00e1lez-Miguel","sequence":"additional","affiliation":[{"name":"Laboratory of Helminth Parasites of Zoonotic Importance (ATENEA), Institute of Natural Resources and Agrobiology of Salamanca (IRNASA-CSIC)","place":["Salamanca, Spain"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2026,9,25]]},"reference":[{"key":"B1","first-page":"1","article-title":"Chapter 1. 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