{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,9]],"date-time":"2026-09-09T13:06:49Z","timestamp":1788959209775,"version":"build-2803163510"},"reference-count":41,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,8,21]],"date-time":"2026-08-21T00:00:00Z","timestamp":1787270400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100012620","name":"Taishan Scholar Foundation of Shandong Province","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100012620","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Cell. Infect. Microbiol."],"abstract":"<jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>To explore the alterations in the microbiota and transcriptome of patients with chronic atrophic gastritis (CAG) and liver cirrhosis (LC) and to develop robust diagnostic models.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>16S ribosomal RNA (rRNA) amplicon sequencing was performed on saliva and gastric mucosal samples, and transcriptome sequencing was performed on gastric mucosal samples from the study participants.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      <jats:italic>Veillonella, Ruminococcus_gnavus_group<\/jats:italic>\n                      ,\n                      <jats:italic>Olsenella<\/jats:italic>\n                      , and other genera were enriched in the saliva of LC patients with CAG (Cirrhosis A) compared with both LC patients without CAG (Cirrhosis N) and controls without LC (Control), while\n                      <jats:italic>Lautropia<\/jats:italic>\n                      was reduced (LDA\u2009&amp;gt;\u20092.0, adjusted\n                      <jats:italic>P<\/jats:italic>\n                      -value &amp;lt;\u20090.05). Moreover, in the independent test set, the combination of\n                      <jats:italic>Cardiobacterium<\/jats:italic>\n                      and\n                      <jats:italic>Olsenella<\/jats:italic>\n                      classified Cirrhosis A subjects from Cirrhosis N subjects with an area under the curve (AUC) of 0.89, and classified Cirrhosis A subjects from Control subjects with an AUC of 0.84. An eight-genera panel achieved an AUC of 0.92 for classifying Cirrhosis A from Control. In the gastric mucosa,\n                      <jats:italic>ABCG5<\/jats:italic>\n                      ,\n                      <jats:italic>ITGA2<\/jats:italic>\n                      ,\n                      <jats:italic>MYB<\/jats:italic>\n                      , and other genes were upregulated, whereas EPOR,\n                      <jats:italic>CHAD, IGFALS<\/jats:italic>\n                      , and other genes were downregulated in Cirrhosis A compared with both Cirrhosis N and CAG patients without LC (Control A) (adjusted\n                      <jats:italic>P<\/jats:italic>\n                      -value &amp;lt; 0.05). The overlapping differentially expressed genes were enriched, including in the PI3K-Akt signaling pathway, bile secretion, cholesterol metabolism, PPAR signaling, and steroid biosynthesis pathways. In Cirrhosis A, correlation analysis revealed that increased\n                      <jats:italic>Aggregatibacter<\/jats:italic>\n                      was positively correlated with\n                      <jats:italic>TTC22<\/jats:italic>\n                      and\n                      <jats:italic>PRSS3<\/jats:italic>\n                      , whereas increased\n                      <jats:italic>Allobaculum<\/jats:italic>\n                      was positively correlated with\n                      <jats:italic>TFAP2B<\/jats:italic>\n                      .\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>LC is associated with distinct microbial and transcriptomic reprogramming in CAG, with enrichment of the PI3K-Akt signaling and bile secretion pathways, suggesting their possible involvement in cirrhosis-associated gastric mucosal changes and warranting further investigation.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.3389\/fcimb.2026.1893991","type":"journal-article","created":{"date-parts":[[2026,8,21]],"date-time":"2026-08-21T04:22:51Z","timestamp":1787286171000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["Integrated microbial and transcriptomic profiling reveals distinct correlations in atrophic gastritis associated with liver cirrhosis"],"prefix":"10.3389","volume":"16","author":[{"given":"Yiqing","family":"Zhu","sequence":"first","affiliation":[{"name":"Department of Gastroenterology, Qilu Hospital of Shandong University","place":["Jinan, Shandong, China"]},{"name":"Shandong Provincial Clinical Research Center for Digestive Disease","place":["Jinan, Shandong, China"]},{"name":"Laboratory of Translational Gastroenterology, Qilu Hospital of Shandong University","place":["Jinan, Shandong, China"]},{"name":"Robot Engineering Laboratory for Precise 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