{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T05:54:41Z","timestamp":1790747681452,"version":"4.1.0"},"reference-count":62,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T00:00:00Z","timestamp":1790726400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Cell. Infect. Microbiol."],"abstract":"<jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>\n                      <jats:italic>Pasteurella aerogenes<\/jats:italic>\n                      is an opportunistic zoonotic pathogen associated with reproductive disorders in animals and sporadic human infections. However, the complete genome, antimicrobial susceptibility profile, and phylogenetic relationships of clinical\n                      <jats:italic>P. aerogenes<\/jats:italic>\n                      isolates, particularly from China, remain poorly understood.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      We sequenced the complete genome of the clinical\n                      <jats:italic>P. aerogenes<\/jats:italic>\n                      isolate 21PA20240911 from a patient with urinary tract infection in Hainan, China, using a hybrid PacBio and Illumina sequencing approach. Comparative genomic analyses, including genome feature characterization, ANI-based species delineation, virulence gene screening, antimicrobial susceptibility testing, and cgSNP-based phylogenetic analysis, were performed to investigate the strains genomic landscape and evolutionary origin.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      The genome of strain 21PA20240911 was 2.3Mbp with a G+C content of 40.84%. ANI analysis confirmed its taxonomic assignment as\n                      <jats:italic>P. aerogenes<\/jats:italic>\n                      (97.61% identity with the reference strain). The strain carried 28 virulence-associated genes. Notably,\n                      <jats:italic>tviB<\/jats:italic>\n                      and\n                      <jats:italic>wbpD<\/jats:italic>\n                      were detected only in our strain and a duck-derived Sichuan isolate. The isolate was resistant only to erythromycin but showed nonsusceptibility to multiple \u03b2-lactams and tetracyclines. Resistance-associated determinants included\n                      <jats:italic>tet (B)\/tetR<\/jats:italic>\n                      and a putative PBP3\/FtsI-like protein variant carrying D350N and S357N substitutions. Phylogenetic analysis tentatively suggests the possibility of zoonotic transmission from local poultry for this strain.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>\n                      This study provides the first complete genome sequence of a clinical\n                      <jats:italic>P. aerogenes<\/jats:italic>\n                      isolate from China, offering a genomic foundation for understanding its pathogenicity and antimicrobial resistance. From a One Health perspective, enhanced surveillance in both clinical and veterinary settings is warranted.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.3389\/fcimb.2026.1900215","type":"journal-article","created":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T04:56:10Z","timestamp":1790744170000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["Genomic and antimicrobial susceptibility characterization of a clinical Pasteurella aerogenes isolate from a human patient"],"prefix":"10.3389","volume":"16","author":[{"given":"Ying","family":"Chen","sequence":"first","affiliation":[{"name":"Laboratory Department, Qionghai People\u2019s Hospital","place":["Qionghai, Hainan, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gerrit","family":"Brandis","sequence":"additional","affiliation":[{"name":"Department of Cell and Molecular Biology, Uppsala University","place":["Uppsala, Sweden"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hong","family":"Li","sequence":"additional","affiliation":[{"name":"Laboratory Department, Qionghai People\u2019s Hospital","place":["Qionghai, Hainan, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yongjun","family":"Luo","sequence":"additional","affiliation":[{"name":"Xinjiang Uygur Autonomous Region Center for Disease Control and Prevention","place":["Urumqi, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huiyu","family":"Wang","sequence":"additional","affiliation":[{"name":"Laboratory Department, Qionghai People\u2019s Hospital","place":["Qionghai, Hainan, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jinrong","family":"He","sequence":"additional","affiliation":[{"name":"National Key Laboratory of Intelligent Tracking and Forecasting for Infectious Disease, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention","place":["Beijing, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yumeng","family":"Wang","sequence":"additional","affiliation":[{"name":"National Key Laboratory of Intelligent Tracking and Forecasting for Infectious Disease, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention","place":["Beijing, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2026,9,30]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gks406","article-title":"PhiSpy: a novel algorithm for finding prophages in bacterial genomes that combines similarity- and composition-based strategies","volume":"40","author":"Akhter","year":"2012","journal-title":"Nucleic Acids Res."},{"key":"B2","doi-asserted-by":"publisher","first-page":"82","DOI":"10.1093\/tropej\/fmx028","article-title":"Pasteurella aerogenes as an asymptomatic bacteriuria agent","volume":"64","author":"Alaygut","year":"2018","journal-title":"J. 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