{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,25]],"date-time":"2026-08-25T15:32:54Z","timestamp":1787671974443,"version":"build-2736575974"},"reference-count":51,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,8,3]],"date-time":"2026-08-03T00:00:00Z","timestamp":1785715200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Cell. Infect. Microbiol."],"abstract":"<jats:sec>\n                    <jats:title>Introduction<\/jats:title>\n                    <jats:p>\n                      Type VI secretion system (T6SS) is a key bacterial secretion device in\n                      <jats:italic>Salmonella enterica<\/jats:italic>\n                      serovar Typhimurium (\n                      <jats:italic>S<\/jats:italic>\n                      . Typhimurium), responsible for translocating effectors to mediate bacterial-host interaction. Tldi1, a well-characterized immunity protein encoded in the T6SS gene cluster, specifically neutralizes the cognate toxin Tlde1 to prevent bacterial \u201cfriendly fire\u201d. However, there is still a lack of systematic and in-depth research on the functions of Tldi1 at present. In order to elucidate the phenotype and toxicity effects of Tldi1 deficiency on\n                      <jats:italic>S<\/jats:italic>\n                      . Typhimurium, this study used\n                      <jats:italic>S<\/jats:italic>\n                      . Typhimurium SL1344 as a model strain and explored the role of Tldi1 toxicity on bacterial ecological adaptability and infection process through multi-level experiments.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      Firstly, molecular bioinformatics approaches were employed to analyze the physicochemical properties, hydrophilicity, hydrophobicity, transmembrane regions, subcellular localization, as well as secondary and tertiary structures of the\u00a0Tldi1 protein. Secondly, a\n                      <jats:italic>tldi1<\/jats:italic>\n                      -deficient mutant of\n                      <jats:italic>S<\/jats:italic>\n                      . Typhimurium was constructed via homologous double crossover recombination, and alterations in its biological characteristics were analyzed. Finally, a mouse infection model was used to investigate the effect of\n                      <jats:italic>tldi1<\/jats:italic>\n                      deletion on the infection progression of\n                      <jats:italic>S<\/jats:italic>\n                      . Typhimurium.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      The results showed that compared with the wild-type (WT) strain, the \u0394\n                      <jats:italic>tldi1<\/jats:italic>\n                      strain exhibited reduced splenic colonization, and regulated host inflammation, while these phenotypes were reversed in the \u0394\n                      <jats:italic>tldi1\/p<\/jats:italic>\n                      \u0394\n                      <jats:italic>tldi1<\/jats:italic>\n                      strain.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Discussion<\/jats:title>\n                    <jats:p>\n                      Tldi1, as a key immunity protein for Tlde1 neutralization, indirectly regulates the environmental adaptability and host immune homeostasis of\n                      <jats:italic>S.<\/jats:italic>\n                      \u00a0Typhimurium, providing new insights into the function of T6SS immunity proteins.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.3389\/fcimb.2026.1902089","type":"journal-article","created":{"date-parts":[[2026,8,3]],"date-time":"2026-08-03T05:29:49Z","timestamp":1785734989000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["Tldi1 deficiency mediates Tlde1 self-toxic effect to regulate the adaptability of Salmonella Typhimurium infection and host immune response in mice"],"prefix":"10.3389","volume":"16","author":[{"given":"Junfeng","family":"Zhang","sequence":"first","affiliation":[{"name":"College of Medical Technology and Engineering, Henan University of Science and Technology","place":["Luoyang, China"]},{"name":"College of Animal Science and Technology\/Laboratory of Functional Microbiology and Animal Health, Henan University of Science and Technology;","place":["Luoyang, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chang","family":"Liu","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology\/Laboratory of Functional Microbiology and Animal Health, Henan University of Science and Technology;","place":["Luoyang, China"]},{"name":"College of Animal Science and Technology, Key Laboratory of Live Carrier Biomaterials and Animal Disease Prevention and Control","place":["Luoyang, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Songbiao","family":"Chen","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology\/Laboratory of Functional Microbiology and Animal Health, Henan University of Science and Technology;","place":["Luoyang, China"]},{"name":"College of Animal Science and Technology, Key Laboratory of Live Carrier Biomaterials and Animal Disease Prevention and Control","place":["Luoyang, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rongxian","family":"Guo","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology\/Laboratory of Functional Microbiology and Animal Health, Henan University of Science and Technology;","place":["Luoyang, China"]},{"name":"College of Animal Science and Technology, Key Laboratory of Live Carrier Biomaterials and Animal Disease Prevention and Control","place":["Luoyang, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanyan","family":"Jia","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology\/Laboratory of Functional Microbiology and Animal Health, Henan University of Science and Technology;","place":["Luoyang, China"]},{"name":"College of Animal Science and Technology, Key Laboratory of Live Carrier Biomaterials and Animal Disease Prevention and Control","place":["Luoyang, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jing","family":"Li","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology\/Laboratory of Functional Microbiology and Animal Health, Henan University of Science and Technology;","place":["Luoyang, China"]},{"name":"College of Animal Science and Technology, Key Laboratory of Live Carrier Biomaterials and Animal Disease Prevention and Control","place":["Luoyang, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chengshui","family":"Liao","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology\/Laboratory of Functional Microbiology and Animal Health, Henan University of Science and Technology;","place":["Luoyang, China"]},{"name":"College of Animal Science and Technology, Key Laboratory of Live Carrier Biomaterials and Animal Disease Prevention and Control","place":["Luoyang, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ke","family":"Ding","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Henan Institute of Science and Technology","place":["Xinxiang, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lei","family":"He","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology\/Laboratory of Functional Microbiology and Animal Health, Henan University of Science and Technology;","place":["Luoyang, China"]},{"name":"College of Animal Science and Technology, Key Laboratory of Live Carrier Biomaterials and Animal Disease Prevention and Control","place":["Luoyang, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ke","family":"Shang","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology\/Laboratory of Functional Microbiology and Animal Health, Henan University of Science and Technology;","place":["Luoyang, China"]},{"name":"College of Animal Science and Technology, Key Laboratory of Live Carrier Biomaterials and Animal Disease Prevention and Control","place":["Luoyang, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2026,8,3]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","DOI":"10.1096\/fasebj.2021.35.s1.01667","article-title":"Structural basis for effector transmembrane domain recognition by type VI secretion system chaperones","volume":"9","author":"Ahmad","year":"2020","journal-title":"eLife"},{"key":"B2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2022.811932","article-title":"Identification of type VI secretion systems effector proteins that contribute to interbacterial competition in Salmonella Dublin","volume":"13","author":"Amaya","year":"2022","journal-title":"Front. Microbiol."},{"key":"B3","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2023.1252344","article-title":"Identification and distribution of new candidate T6SS effectors encoded in Salmonella Pathogenicity Island 6","volume":"14","author":"Blondel","year":"2023","journal-title":"Front. Microbiol."},{"key":"B4","doi-asserted-by":"publisher","DOI":"10.1007\/s00262-023-03503-6","article-title":"Deciphering the Salmonella T6SS toolkit: two decades of research decoding a versatile bacterial weapon","volume":"207","author":"Blondel","year":"2025","journal-title":"J. Bacteriol."},{"key":"B5","doi-asserted-by":"publisher","first-page":"19155","DOI":"10.1021\/acs.jafc.4c03735","article-title":"Colonization mediated by T6SS-ClpV disrupts host gut microbiota and enhances virulence of Salmonella enterica serovar Typhimurium","volume":"72","author":"Chen","year":"2024","journal-title":"J. Agric. Food. Chem."},{"key":"B6","doi-asserted-by":"publisher","first-page":"105277","DOI":"10.1016\/j.psj.2025.105277","article-title":"Salmonella enterica serovar Typhimurium uses T6SS to promote bacterial adaptability and enhance its survival of niches in broiler chickens","volume":"104","author":"Chen","year":"2025","journal-title":"Poult. Sci."},{"key":"B7","doi-asserted-by":"publisher","first-page":"72","DOI":"10.1186\/s12866-015-0405-9","article-title":"A Pseudomonas fluorescens type 6 secretion system is related to mucoidy, motility and bacterial competition","volume":"15","author":"Decoin","year":"2015","journal-title":"BMC Microbiol."},{"key":"B8","doi-asserted-by":"publisher","DOI":"10.1128\/spectrum.02465-22","article-title":"A DNase type VI secretion system effector requires its mix domain for secretion","volume":"10","author":"Fridman","year":"2022","journal-title":"Microbiol. Spectr."},{"key":"B9","doi-asserted-by":"publisher","first-page":"104900","DOI":"10.4135\/9781526466334","article-title":"Rapid detection of Salmonella Enteritidis and S. Typhimurium serotypes and characterization of flagellar variants using real-time PCR","volume":"133","author":"Fr\u00f6der","year":"2026","journal-title":"Food Microbiol."},{"key":"B10","doi-asserted-by":"publisher","DOI":"10.1080\/21505594.2025.2548621","article-title":"Isopropyl paraben targets type III secretion to inhibit Salmonella enterica serovar typhimurium infection","volume":"16","author":"Ge","year":"2025","journal-title":"Virulence"},{"key":"B11","doi-asserted-by":"publisher","DOI":"10.1128\/mbio.00744-13","article-title":"Protein domains of unknown function are essential in bacteria","volume":"5","author":"Goodacre","year":"2013","journal-title":"mBio"},{"key":"B12","doi-asserted-by":"publisher","first-page":"144","DOI":"10.1016\/j.rvsc.2022.10.016","article-title":"Acetoacetyl-CoA transferase ydiF regulates the biofilm formation of avian pathogenic Escherichia coli","volume":"153","author":"Gu","year":"2022","journal-title":"Res. Vet. Sci."},{"key":"B13","doi-asserted-by":"publisher","first-page":"113925","DOI":"10.1016\/j.celrep.2024.113925","article-title":"Salmonella T3SS-2 virulence enhances gut-luminal colonization by enabling chemotaxis-dependent exploitation of intestinal inflammation","volume":"43","author":"G\u00fcl","year":"2024","journal-title":"Cell Rep."},{"key":"B14","doi-asserted-by":"publisher","first-page":"4","DOI":"10.1186\/s13099-019-0283-z","article-title":"Campylobacter jejuni enters gut epithelial cells and impairs intestinal barrier function through cleavage of occludin by serine protease HtrA","volume":"11","author":"Harrer","year":"2019","journal-title":"Gut. Pathog."},{"key":"B15","doi-asserted-by":"publisher","first-page":"13114","DOI":"10.1093\/nar\/gkac1162","article-title":"Salmonella antibacterial Rhs polymorphic toxin inhibits translation through ADP-ribosylation of EF-Tu P-loop","volume":"50","author":"Jur\u0117nas","year":"2022","journal-title":"Nucleic Acids Res."},{"key":"B16","doi-asserted-by":"publisher","first-page":"2375","DOI":"10.1016\/j.str.2024.10.008","article-title":"T6SS-associated Rhs toxin-encapsulating shells: Structural and bioinformatical insights into bacterial weaponry and self-protection","volume":"32","author":"Kielkopf","year":"2024","journal-title":"Structure. (Lond. Engl. 1993)"},{"key":"B17","doi-asserted-by":"publisher","DOI":"10.3390\/ani13182824","article-title":"Phenotype alterations in the cecal ecosystem involved in the asymptomatic intestinal persistence of paratyphoid Salmonella in chickens","volume":"13","author":"Kogut","year":"2023","journal-title":"Anim. An. Open Access J. MDPI."},{"key":"B18","doi-asserted-by":"publisher","DOI":"10.3390\/ijms24021740","article-title":"Activity and crystal structure of the adherent-invasive Escherichia coli Tle3\/Tli3 T6SS effector\/immunity complex determined using an AlphaFold2 Predicted Model","volume":"24","author":"Le","year":"2023","journal-title":"Int. J. Mol. Sci."},{"key":"B19","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2020.01096","article-title":"ClpV3 of the H3-Type VI secretion system (H3-T6SS) affects multiple virulence factors in Pseudomonas aeruginosa","volume":"11","author":"Li","year":"2020","journal-title":"Front. Microbiol."},{"key":"B20","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2024.1379106","article-title":"TagP, a PAAR-domain containing protein, plays roles in the fitness and virulence of Acinetobacter baumannii","volume":"14","author":"Li","year":"2024","journal-title":"Front. Cell. Infect. Microbiol."},{"key":"B21","doi-asserted-by":"publisher","DOI":"10.3390\/ijms24021614","article-title":"Pseudomonas aeruginosa H3-T6SS combats H(2)O(2) stress by diminishing the amount of intracellular unincorporated iron in a dps-dependent manner and inhibiting the synthesis of PQS","volume":"24","author":"Lin","year":"2023","journal-title":"Int. J. Mol. Sci."},{"key":"B22","doi-asserted-by":"publisher","first-page":"73","DOI":"10.1111\/j.1472-765x.2012.03263.x","article-title":"Investigation of the roles of T6SS genes in motility, biofilm formation, and extracellular protease Asp production in Vibrio alginolyticus with modified Gateway-compatible plasmids","volume":"55","author":"Liu","year":"2012","journal-title":"Lett. Appl. Microbiol."},{"key":"B23","doi-asserted-by":"crossref","first-page":"2241204","DOI":"10.1080\/19490976.2023.2241204","article-title":"The phospholipase effector Tle1(Vc) promotes Vibrio cholerae virulence by killing competitors and impacting gene expression","volume":"15","author":"Liu","year":"2023","journal-title":"Gut. Microbes"},{"key":"B24","doi-asserted-by":"publisher","DOI":"10.1016\/j.jbc.2022.102556","article-title":"Molecular characterization of the type VI secretion system effector Tlde1a reveals a structurally altered LD-transpeptidase fold","volume":"298","author":"Lorente Cobo","year":"2022","journal-title":"J. Biol. Chem."},{"key":"B25","doi-asserted-by":"publisher","DOI":"10.3390\/ijms24044187","article-title":"Unraveling the diverse roles of neglected genes containing domains of unknown function (DUFs): progress and perspective","volume":"24","author":"Lv","year":"2023","journal-title":"Int. J. Mol. Sci."},{"key":"B26","doi-asserted-by":"publisher","first-page":"1019","DOI":"10.1046\/j.1365-2958.2002.03070.x","article-title":"Conflicting needs for a Salmonella hypervirulence gene in host and non-host environments","volume":"45","author":"Mouslim","year":"2002","journal-title":"Mol. Microbiol."},{"key":"B27","doi-asserted-by":"publisher","first-page":"1996","DOI":"10.1128\/iai.06205-11","article-title":"Type VI secretion system-associated gene clusters contribute to pathogenesis of Salmonella enterica serovar Typhimurium","volume":"80","author":"Mulder","year":"2012","journal-title":"Infect. Immun."},{"key":"B28","doi-asserted-by":"publisher","first-page":"4338","DOI":"10.1128\/IAI.73.7.4338-4345.2005","article-title":"sciS, an icmF homolog in Salmonella enterica serovar Typhimurium, limits intracellular replication and decreases virulence","volume":"73","author":"Parsons","year":"2005","journal-title":"Infect. Immun."},{"key":"B29","doi-asserted-by":"publisher","first-page":"128","DOI":"10.1016\/j.molcel.2010.03.001","article-title":"The c-di-GMP binding protein YcgR controls flagellar motor direction and speed to affect chemotaxis by a \"backstop brake\" mechanism","volume":"38","author":"Paul","year":"2010","journal-title":"Mol. Cell"},{"key":"B30","doi-asserted-by":"publisher","first-page":"E5044","DOI":"10.1073\/pnas.1608858113","article-title":"Salmonella Typhimurium utilizes a T6SS-mediated antibacterial weapon to establish in the host gut","volume":"113","author":"Sana","year":"2016","journal-title":"PNAS"},{"key":"B31","doi-asserted-by":"publisher","first-page":"652","DOI":"10.1016\/j.vetmic.2012.09.009","article-title":"Connecting type VI secretion, quorum sensing, and c-di-GMP production in fish pathogen Vibrio alginolyticus through phosphatase PppA","volume":"162","author":"Sheng","year":"2013","journal-title":"Vet. Microbiol."},{"key":"B32","doi-asserted-by":"publisher","DOI":"10.1016\/j.anaerobe.2024.102819","article-title":"Bacteroides and related species: The keystone taxa of the human gut microbiota","volume":"85","author":"Shin","year":"2024","journal-title":"Anaerobe"},{"key":"B33","doi-asserted-by":"publisher","first-page":"107813","DOI":"10.1099\/acmi.ac2020.po0033","article-title":"A family of T6SS antibacterial effectors related to l,d-transpeptidases targets the peptidoglycan","volume":"31","author":"Sibinelli-Sousa","year":"2020","journal-title":"Cell Rep."},{"key":"B34","doi-asserted-by":"publisher","first-page":"76","DOI":"10.1186\/s12866-020-01743-5","article-title":"Inactivation of the T6SS inner membrane protein DotU results in severe attenuation and decreased pathogenicity of Aeromonas veronii TH0426","volume":"20","author":"Song","year":"2020","journal-title":"BMC Microbiol."},{"key":"B35","doi-asserted-by":"publisher","first-page":"423","DOI":"10.1038\/s41467-020-20726-8","article-title":"Contact-independent killing mediated by a T6SS effector with intrinsic cell-entry properties","volume":"12","author":"Song","year":"2021","journal-title":"Nat. Commun."},{"key":"B36","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2022.948237","article-title":"The salmonella effector Hcp modulates infection response, and affects salmonella adhesion and egg contamination incidences in ducks","volume":"12","author":"Song","year":"2022","journal-title":"Front. Cell. Infect. Microbiol."},{"key":"B37","doi-asserted-by":"publisher","DOI":"10.1002\/advs.202504086","article-title":"A dual-targeting T6SS DNase drives bacterial antagonism and eukaryotic apoptosis via the cgas-sting-tnf axis","volume":"12","author":"Song","year":"2025","journal-title":"Adv. Sci. (Weinheim. Baden-Wurttemberg. Germany)."},{"key":"B38","doi-asserted-by":"publisher","first-page":"1262","DOI":"10.1111\/mmi.12432","article-title":"Cyclic di-GMP inhibits Vibrio cholerae motility by repressing induction of transcription and inducing extracellular polysaccharide production","volume":"90","author":"Srivastava","year":"2013","journal-title":"Mol. Microbiol."},{"key":"B39","doi-asserted-by":"publisher","first-page":"585","DOI":"10.1080\/21505594.2017.1421829","article-title":"A type 6 secretion system (T6SS) encoded gene within Salmonella enterica serovar Enteritidis contributes to virulence","volume":"9","author":"Troxell","year":"2018","journal-title":"Virulence"},{"key":"B40","doi-asserted-by":"publisher","DOI":"10.1016\/j.micres.2025.128105","article-title":"Simultaneous functional disruption of the iron acquisition system and type VI secretion system results in complete suppression of virulence in Acinetobacter baumannii","volume":"295","author":"Wang","year":"2025","journal-title":"Microbiol. Res."},{"key":"B41","doi-asserted-by":"publisher","first-page":"1716","DOI":"10.1080\/21505594.2020.1854538","article-title":"Roles of the Hcp family proteins in the pathogenicity of Salmonella typhimurium 14028s","volume":"11","author":"Wang","year":"2020","journal-title":"Virulence"},{"key":"B42","doi-asserted-by":"publisher","first-page":"3390","DOI":"10.3168\/jds.2024-25685","article-title":"OmpR-mediated activation of the type Vl secretion system drives enhanced acid tolerance in Cronobacter","volume":"108","author":"Wang","year":"2025","journal-title":"J. Dairy. Sci."},{"key":"B43","doi-asserted-by":"publisher","first-page":"252","DOI":"10.1128\/msphere.00771-23","article-title":"Competitive behaviors in Serratia marcescens are coordinately regulated by a lifestyle switch frequently inactivated in the clinical environment","volume":"33","author":"Williams","year":"2025","journal-title":"Cell. Host Microbe"},{"key":"B44","doi-asserted-by":"publisher","first-page":"55","DOI":"10.1007\/s44154-022-00078-7","article-title":"H3-T6SS of Pseudomonas aeruginosa PA14 contributes to environmental adaptation via secretion of a biofilm-promoting effector","volume":"2","author":"Yang","year":"2022","journal-title":"Stress Biol."},{"key":"B45","doi-asserted-by":"publisher","first-page":"e00427","DOI":"10.1128\/jb.00427-20","article-title":"The transcription regulator and c-di-GMP phosphodiesterase PdeL represses motility in Escherichia coli","volume":"203","author":"Yilmaz","year":"2020","journal-title":"J. Bacteriol."},{"key":"B46","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0073782","article-title":"Insights into the cross-immunity mechanism within effector families of bacteria type VI secretion system from the structure of StTae4-EcTai4 complex","volume":"8","author":"Zhang","year":"2013","journal-title":"PloS One"},{"key":"B47","doi-asserted-by":"publisher","DOI":"10.1016\/j.bios.2025.117895","article-title":"PfAgo-DNAzyme cascade amplification improves Salmonella typhimurium detection","volume":"289","author":"Zheng","year":"2025","journal-title":"Biosens. Bioelectron."},{"key":"B48","doi-asserted-by":"publisher","DOI":"10.1128\/aem.01655-21","article-title":"The two-component system FleS\/FleR represses H1-T6SS via cyclic di-GMP signaling in Pseudomonas aeruginosa","volume":"88","author":"Zhou","year":"2022","journal-title":"Appl. Environ. Microbiol."},{"key":"B49","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2103526118","article-title":"T6SS translocates a micropeptide to suppress STING-mediated innate immunity by sequestering manganese","volume":"118","author":"Zhu","year":"2021","journal-title":"PNAS"},{"key":"B50","doi-asserted-by":"publisher","DOI":"10.3390\/biology14070822","article-title":"The regulatory role of evfg through coordinated control of flagellar biosynthesis and energy metabolism in porcine extraintestinal pathogenic Escherichia coli (ExPEC)","volume":"14","author":"Zong","year":"2025","journal-title":"Biology"},{"key":"B51","doi-asserted-by":"publisher","DOI":"10.1007\/s44154-022-00081-y","article-title":"A Fur-regulated type VI secretion system contributes to oxidative stress resistance and virulence in Yersinia pseudotuberculosis","volume":"3","author":"Zuo","year":"2023","journal-title":"Stress Biol."}],"updated-by":[{"DOI":"10.3389\/fcimb.2026.1963209","type":"correction","label":"Correction","source":"publisher","updated":{"date-parts":[[2026,8,25]],"date-time":"2026-08-25T00:00:00Z","timestamp":1787616000000}}],"container-title":["Frontiers in Cellular and Infection Microbiology"],"original-title":[],"link":[{"URL":"https:\/\/www.frontiersin.org\/articles\/10.3389\/fcimb.2026.1902089\/full","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,25]],"date-time":"2026-08-25T14:54:50Z","timestamp":1787669690000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.frontiersin.org\/articles\/10.3389\/fcimb.2026.1902089\/full"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,8,3]]},"references-count":51,"alternative-id":["10.3389\/fcimb.2026.1902089"],"URL":"https:\/\/doi.org\/10.3389\/fcimb.2026.1902089","relation":{},"ISSN":["2235-2988"],"issn-type":[{"value":"2235-2988","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,8,3]]},"article-number":"1902089"}}