{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T11:52:56Z","timestamp":1791373976503,"version":"4.3.1"},"reference-count":24,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,8,27]],"date-time":"2026-08-27T00:00:00Z","timestamp":1787788800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Cell. Infect. Microbiol."],"abstract":"<jats:p>\n                    Zika virus (ZIKV) can persist in mosquito populations through both horizontal and vertical transmission, and successful ovarian infection is a prerequisite for transgenerational spread. However, the mosquito host factors that regulate ovarian permissiveness and vertical transmission remain poorly characterized. Here, we investigated the role of cytochrome P450 4G15 (\n                    <jats:italic>CYP4G15<\/jats:italic>\n                    ) in ZIKV infection and vertical transmission in\n                    <jats:italic>Aedes albopictus<\/jats:italic>\n                    and identified an associated downstream pathway involving\n                    <jats:italic>SORD-like<\/jats:italic>\n                    , a predicted sorbitol dehydrogenase-like gene. Expression profiling revealed that\n                    <jats:italic>CYP4G15<\/jats:italic>\n                    exhibits distinct tissue-, developmental-, blood-feeding-, and infection-responsive patterns, with transient induction in ovaries during the early phase of ZIKV infection. RNA interference-mediated silencing of\n                    <jats:italic>CYP4G15<\/jats:italic>\n                    , followed by transcriptomic analysis and RT-qPCR validation, identified\n                    <jats:italic>SORD-like<\/jats:italic>\n                    (LOC109415972) as a candidate\n                    <jats:italic>CYP4G15<\/jats:italic>\n                    -associated gene.\n                    <jats:italic>SORD-like<\/jats:italic>\n                    was highly expressed in the fat body, strongly induced after blood feeding, and responsive to ZIKV challenge in ovarian tissues. Functional assays showed that knockdown of\n                    <jats:italic>SORD-like<\/jats:italic>\n                    significantly reduced early ovarian infection rate and viral RNA accumulation, while also decreasing conditional progeny egg-pool positivity rate and minimum egg infection rate, indicating reduced ZIKV RNA detection in progeny egg pools.\n                    <jats:italic>SORD-like<\/jats:italic>\n                    silencing did not alter\n                    <jats:italic>CYP4G15<\/jats:italic>\n                    transcript levels, whereas\n                    <jats:italic>CYP4G15<\/jats:italic>\n                    overexpression was associated with increased\n                    <jats:italic>SORD-like<\/jats:italic>\n                    transcript abundance and functionally compensated for the ovarian viral RNA phenotype caused by\n                    <jats:italic>SORD-like<\/jats:italic>\n                    knockdown. Together, these findings support a functional association between\n                    <jats:italic>CYP4G15, SORD-like<\/jats:italic>\n                    , and ovarian ZIKV infection, but do not establish direct transcriptional regulation or a strict linear epistatic relationship. Our findings reveal a\n                    <jats:italic>CYP4G15<\/jats:italic>\n                    \u2013\n                    <jats:italic>SORD-lik<\/jats:italic>\n                    e-associated metabolic-regulatory pathway that links mosquito physiology to arbovirus persistence. Given that\n                    <jats:italic>CYP4G<\/jats:italic>\n                    -related pathways are often associated with environmental adaptation and insecticide resistance-associated physiology, this pathway may represent an important point of intersection between vector fitness and viral transmission. It may therefore serve as a candidate target for reducing egg-associated ZIKV RNA detection and ZIKV maintenance in\n                    <jats:italic>Aedes albopictus<\/jats:italic>\n                    populations.\n                  <\/jats:p>","DOI":"10.3389\/fcimb.2026.1910501","type":"journal-article","created":{"date-parts":[[2026,8,27]],"date-time":"2026-08-27T04:27:43Z","timestamp":1787804863000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["The CYP4G15-SORD-like-associated pathway promotes ovarian ZIKV infection and egg-associated viral RNA detection in Aedes albopictus"],"prefix":"10.3389","volume":"16","author":[{"given":"Mei","family":"Chen","sequence":"first","affiliation":[{"name":"School of Public Health, Southern Medical University","place":["Guangzhou, Guangdong, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yajing","family":"Zhang","sequence":"additional","affiliation":[{"name":"Center of Laboratory Medicine, Shenzhen Nanshan People\u2019s Hospital (Affiliated Sixth Hospital of Shenzhen University)","place":["Shenzhen, Guangdong, China"]},{"name":"Medical Laboratory, The Third Affiliated Hospital of Shenzhen University","place":["Shenzhen, Guangdong, China"]},{"name":"College of Laboratory Medicine, Dalian Medical University","place":["Dalian, Liaoning, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenwei","family":"Zhong","sequence":"additional","affiliation":[{"name":"Center of Laboratory Medicine, Shenzhen Nanshan People\u2019s Hospital (Affiliated Sixth Hospital of Shenzhen University)","place":["Shenzhen, Guangdong, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mei","family":"Hong","sequence":"additional","affiliation":[{"name":"School of Public Health, Southern Medical University","place":["Guangzhou, Guangdong, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jinbao","family":"Gu","sequence":"additional","affiliation":[{"name":"School of Public Health, Southern Medical University","place":["Guangzhou, Guangdong, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zetian","family":"Lai","sequence":"additional","affiliation":[{"name":"Center of Laboratory Medicine, Shenzhen Nanshan People\u2019s Hospital (Affiliated Sixth Hospital of Shenzhen University)","place":["Shenzhen, Guangdong, China"]},{"name":"Medical Laboratory, The Third Affiliated Hospital of Shenzhen University","place":["Shenzhen, Guangdong, China"]},{"name":"College of Laboratory Medicine, Dalian Medical University","place":["Dalian, Liaoning, China"]},{"name":"Guangdong Provincial Key Laboratory of Infection Immunity & Inflammation, Department of Pathogen Biology, Shenzhen University Medical School","place":["Shenzhen, Guangdong, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2026,8,27]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"201","DOI":"10.1083\/jcb.50.1.201","article-title":"Permeability of the ovarian follicle of Aedes aEgypti mosquitoes","volume":"50","author":"Anderson","year":"1971","journal-title":"J. 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