{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,21]],"date-time":"2026-08-21T05:23:23Z","timestamp":1787289803841,"version":"build-2736575974"},"reference-count":37,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,8,21]],"date-time":"2026-08-21T00:00:00Z","timestamp":1787270400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Cell. Infect. Microbiol."],"abstract":"<jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Inflammatory bowel disease (IBD) is a chronic recurrent disorder. Gut microbiota-derived metabolites regulate intestinal homeostasis, but their molecular mechanisms in IBD remain unclear. Current studies lack systematic \u201cmicrobiota-metabolite-target\u201d network mining with multi-method validation. This study integrates network pharmacology, three machine learning algorithms, and molecular docking to construct this regulatory network in IBD.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      Transcriptome data were obtained from the Gene Expression Omnibus (GEO) database. Differentially expressed genes (DEGs) were identified using limma (p &amp;lt; 0.05, |log\n                      <jats:sub>2<\/jats:sub>\n                      FC| &amp;gt; 0.5). Weighted gene co-expression network analysis (WGCNA) with an optimal soft threshold of \u03b2 = 7 was performed to identify key module genes. Candidate genes were obtained by intersecting DEGs, gut microbiota-associated genes from the gutMGene database, and WGCNA module genes. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses were conducted to explore the functional roles of candidate genes. Core genes were identified using three machine learning algorithms (LASSO, Boruta, and SVM-RFE), followed by protein-protein interaction (PPI) network analysis. Molecular docking was performed to assess the binding affinities between hub proteins and gut microbiota-derived metabolites.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      A total of 885 DEGs were identified between the IBD and control groups, including 463 upregulated and 422 downregulated genes. WGCNA identified 280 key module genes from the purple and yellow modules. The intersection of DEGs, gut microbiota-associated genes, and WGCNA module genes yielded 19 core candidate genes. PPI network analysis combined with three machine learning algorithms jointly identified C5AR1, KDM2A, and HCAR3 as core hub genes. ROC curve analysis demonstrated that all three hub genes achieved AUC values greater than 0.7 in both the training and validation sets, indicating excellent diagnostic performance for IBD. Enrichment analysis revealed significant associations with the TNF, NF-\u03baB, and IL-17 signaling pathways. Molecular docking confirmed stable binding of C5AR1 with 1,3-Diphenylpropan-2-Ol (-7.87 \u00b1 0.83 kcal\u00b7mol\n                      <jats:sup>-<\/jats:sup>\n                      \u00b9) and HCAR3 with 3-Indolepropionic Acid (-6.35 \u00b1 0.70 kcal\u00b7mol\n                      <jats:sup>-<\/jats:sup>\n                      \u00b9), both below \u22125.0 kcal\u00b7mol\n                      <jats:sup>-<\/jats:sup>\n                      \u00b9.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>This study first constructs a \u201cgut microbiota-metabolite-hub gene\u201d axis in IBD, providing a computational framework for microbiota-targeted precision therapy, and identifying C5AR1\/KDM2A\/HCAR3 as computationally predicted diagnostic biomarkers and 1,3-Diphenylpropan-2-Ol\/3-Indolepropionic Acid as candidate intervention molecules that warrant further experimental validation.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.3389\/fcimb.2026.1912373","type":"journal-article","created":{"date-parts":[[2026,8,21]],"date-time":"2026-08-21T04:23:25Z","timestamp":1787286205000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["Gut microbiota-derived metabolites target C5AR1\/KDM2A\/HCAR3 axis in inflammatory bowel disease: a multi-machine learning algorithms and molecular docking study"],"prefix":"10.3389","volume":"16","author":[{"given":"Shanshan","family":"Hu","sequence":"first","affiliation":[{"name":"Department of Gastroenterology, Henan Provincial People\u2019s Hospital\/People\u2019s Hospital of Zhengzhou University\/People\u2019s Hospital of Henan University","place":["Zhengzhou, Henan, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lin","family":"Fu","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology, Henan Provincial People\u2019s Hospital\/People\u2019s Hospital of Zhengzhou University\/People\u2019s Hospital of Henan University","place":["Zhengzhou, Henan, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jing","family":"Gao","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology, Henan Provincial People\u2019s Hospital\/People\u2019s Hospital of Zhengzhou University\/People\u2019s Hospital of Henan University","place":["Zhengzhou, Henan, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qiongya","family":"Guo","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology, Henan Provincial People\u2019s Hospital\/People\u2019s Hospital of Zhengzhou University\/People\u2019s Hospital of Henan University","place":["Zhengzhou, Henan, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuangyin","family":"Han","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology, Henan Provincial People\u2019s Hospital\/People\u2019s Hospital of Zhengzhou University\/People\u2019s Hospital of Henan University","place":["Zhengzhou, Henan, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2026,8,21]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"411","DOI":"10.1016\/j.pop.2023.03.009","article-title":"Inflammatory bowel disease","volume":"50","author":"Bruner","year":"2023","journal-title":"Prim Care"},{"key":"B2","doi-asserted-by":"publisher","DOI":"10.3389\/fmed.2021.765474","article-title":"Treatment of inflammatory bowel disease: a comprehensive review","volume":"8","author":"Cai","year":"2021","journal-title":"Front. 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