{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,14]],"date-time":"2026-09-14T05:04:52Z","timestamp":1789362292704,"version":"build-2803163510"},"reference-count":27,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,9,14]],"date-time":"2026-09-14T00:00:00Z","timestamp":1789344000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100018537","name":"National Science and Technology Major Project","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100018537","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Cell. Infect. Microbiol."],"abstract":"<jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Dengue virus (DENV) is considered an emerging threat to blood safety. While transfusion-transmitted DENV (TT-DENV) cases have been documented, TT-DENV has never been reported in Chinese mainland. In this study, a retrospective investigation was conducted after two patients developed dengue fever (DF) following apheresis platelet (PLT) transfusion from the same healthy donor.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Blood samples from the recipients and the donor were tested for DENV RNA by nucleic acid testing (NAT). Nonstructural protein 1 (NS1) antigen and anti-DENV immunoglobulin M\/G (IgM\/IgG) antibodies were detected by ELISA. To confirm transmission, DENV E gene fragments were amplified, and whole-genome sequences were obtained by metagenomic next-generation sequencing (mNGS).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Our results revealed that the PLT donor was asymptomatic, tested positive for DENV RNA, and negative for anti-DENV IgM\/IgG antibodies at donation. After donation, no dengue-like symptoms was developed. Seroconversion was observed at day 21 postdonation. After receiving the DENV-positive PLT unit, recipient 1 had DF only, while recipient 2 developed DF together with headache, joint\/muscle pain, and rash. After transfusion, both recipients were positive for DENV RNA and NS1 while negative for anti-DENV IgM\/IgG antibodies. Viral sequences from the donor and recipients were identical and belonged to DENV-2.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>To our knowledge, this is the first TT-DENV case reported in the Chinese mainland, revealing the necessity of revising current donor deferral policies and testing strategies that predominantly rely on symptoms and travel history.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.3389\/fcimb.2026.1920581","type":"journal-article","created":{"date-parts":[[2026,9,14]],"date-time":"2026-09-14T04:39:04Z","timestamp":1789360744000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["Molecular confirmation of transfusion-transmitted dengue virus infection following platelet transfusion in the Chinese 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