{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T05:01:10Z","timestamp":1790830870741,"version":"4.1.0"},"reference-count":30,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:00:00Z","timestamp":1790812800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Cell. Infect. Microbiol."],"abstract":"<jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Vertical transmission is a major driver of new Chagas disease cases. Early diagnosis in newborns is essential for effective treatment; however, conventional screening limitations and high patient attrition lead to severe under-registration. Simplified molecular tools can substantially improve early detection and treatment access.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methodology\/principal findings<\/jats:title>\n                    <jats:p>\n                      We developed a simplified loop-mediated isothermal amplification (LAMP)-based assay targeting the repetitive satellite DNA of\n                      <jats:italic>Trypanosoma cruzi<\/jats:italic>\n                      for qualitative detection in newborn dried blood spots (DBS). Tailored for resource-limited settings, the protocol requires only a dry bath incubator, utilizing lateral flow dipsticks (LFD) for visual readout. The DBS sample is loaded directly into the reaction without nucleic acid extraction. Using artificially spiked DBS, the limit of detection was established at 1 fg of DNA and 1 parasite per reaction. Inclusivity was confirmed across all six\n                      <jats:italic>T. cruzi<\/jats:italic>\n                      discrete typing units (DTUs TcI\u2013TcVI), with no cross-reactivity against heterologous or human DNA. A clinical proof-of-concept evaluation on a blinded panel of 24 newborn DBS samples (4 positive, 20 negative) achieved 100% concordance with definitive clinical status. Notably, the assay successfully identified all 4 congenital cases, including 3 that had tested negative by conventional parasitological microscopy at birth.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions\/significance<\/jats:title>\n                    <jats:p>This simplified, extraction-free molecular test requires minimal equipment and is fully compatible with routine newborn screening infrastructure. It provides a robust, translatable tool for early congenital Chagas diagnosis in decentralized healthcare settings, with high potential to improve detection rates and secure timely access to etiological treatment.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.3389\/fcimb.2026.1934037","type":"journal-article","created":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T04:51:17Z","timestamp":1790830277000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["Extraction-free LAMP-LFD assay for early diagnosis of congenital Chagas disease directly from neonatal dried blood spots"],"prefix":"10.3389","volume":"16","author":[{"given":"Luciana","family":"Larocca","sequence":"first","affiliation":[{"name":"Instituto de Ciencia y Tecnolog\u00eda \u201cDr. C\u00e9sar Milstein\u201d, Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas (CONICET) - Fundaci\u00f3n Cassar\u00e1","place":["Buenos Aires, Argentina"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fabiana G.","family":"Stolowicz","sequence":"additional","affiliation":[{"name":"Instituto de Ciencia y Tecnolog\u00eda \u201cDr. C\u00e9sar Milstein\u201d, Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas (CONICET) - Fundaci\u00f3n Cassar\u00e1","place":["Buenos Aires, Argentina"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matilde","family":"Abudara","sequence":"additional","affiliation":[{"name":"Laboratorio Central, Hospital General de Agudos \u201cDr. Carlos G. Durand\u201d","place":["Buenos Aires, Argentina"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Esteban L.","family":"Figueroa","sequence":"additional","affiliation":[{"name":"Administraci\u00f3n Nacional de Laboratorios e Institutos de Salud (ANLIS) \u201cDr. Carlos Malbr\u00e1n\u201d, Ministerio de Salud de la Naci\u00f3n","place":["Buenos Aires, Argentina"]},{"name":"Facultad de Medicina, Universidad Cat\u00f3lica Argentina","place":["Buenos Aires, Argentina"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gustavo","family":"Maccallini","sequence":"additional","affiliation":[{"name":"Laboratorio Central, Hospital General de Agudos \u201cDr. Carlos G. 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