{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T05:01:20Z","timestamp":1790226080584,"version":"4.1.0"},"reference-count":56,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T00:00:00Z","timestamp":1790208000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Cell. Infect. Microbiol."],"abstract":"<jats:p>Prion diseases are fatal neurodegenerative disorders in which early molecular responses to prion exposure remain incompletely understood, particularly in naturally susceptible hosts. In the present study, we performed RNA-sequencing analysis of ovine mesenchymal stem cell-derived neuron-like cells exposed to classical scrapie brain homogenate and compared their transcriptomic response with that of cells exposed to normal brain homogenate. Cells were analyzed at 2 and 4 days post-inoculation (dpi). Differential expression analysis identified 76 genes at 2 dpi and 124 genes at 4 dpi, with limited overlap between time points, indicating a dynamic response to scrapie exposure. At 2 dpi, enriched biological processes among upregulated genes were mainly related to signaling- and immune-associated transcriptional responses, whereas downregulated genes were enriched in developmental, morphogenetic and lipid-related processes. At 4 dpi, the transcriptional profile was dominated by broader gene repression and showed reduced functional specificity. RT-qPCR analysis of eight selected genes supported the direction of change detected by RNA-seq in most cases. Overall, these results indicate that scrapie exposure elicits a time-dependent transcriptional response in ovine MSC-derived neuron-like cells and provide an exploratory framework for studying early molecular responses associated with prion exposure in a naturally susceptible species.<\/jats:p>","DOI":"10.3389\/fcimb.2026.1938678","type":"journal-article","created":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T04:30:42Z","timestamp":1790224242000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["Time-dependent transcriptomic response to scrapie exposure in ovine MSC-derived neuron-like cells"],"prefix":"10.3389","volume":"16","author":[{"given":"Adelaida","family":"Hernaiz","sequence":"first","affiliation":[{"name":"Laboratorio de Gen\u00e9tica Bioqu\u00edmica (LAGENBIO), Facultad de Veterinaria, Universidad de Zaragoza","place":["Zaragoza, Spain"]},{"name":"Instituto Universitario de Investigaci\u00f3n Mixto 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