{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T05:00:02Z","timestamp":1790312402886,"version":"4.1.0"},"reference-count":63,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T00:00:00Z","timestamp":1790294400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Cell. Infect. Microbiol."],"abstract":"<jats:p>\n                    <jats:italic>Vibrio parahaemolyticus<\/jats:italic>\n                    is a Gram-negative pathogen widely distributed in marine environments and can cause infections in humans and aquatic animals. Type III secretion system 1 (T3SS1) accessory protein VscW is homologous to YscW from\n                    <jats:italic>Yersinia<\/jats:italic>\n                    , which contributes to the stability of the T3SS outer membrane secretin YscC and facilitates its oligomerization and localization to the outer membrane. However, the specific function of\n                    <jats:italic>vscW<\/jats:italic>\n                    in\n                    <jats:italic>V. parahaemolyticus<\/jats:italic>\n                    remains poorly understood. In this study, a\n                    <jats:italic>vscW<\/jats:italic>\n                    deletion mutant (\u0394\n                    <jats:italic>vscW<\/jats:italic>\n                    -P2C) was constructed by homologous recombination. Compared with the wild-type strain (WT-P2C), \u0394\n                    <jats:italic>vscW<\/jats:italic>\n                    -P2C exhibited 41.1% and 45.0% reductions in swimming motility and biofilm formation, respectively. N-phenyl-1-naphthylamine (NPN) fluorescence intensity increased by 60.7% in \u0394\n                    <jats:italic>vscW<\/jats:italic>\n                    -P2C, indicating enhanced outer membrane permeability. Pathogenicity examination showed that the cumulative mortality of\n                    <jats:italic>Litopenaeus vannamei<\/jats:italic>\n                    decreased from 66.7% to 41.7% after deletion of the\n                    <jats:italic>vscW<\/jats:italic>\n                    gene, representing an approximate 37.5% reduction in mortality. Proteomic analysis identified 243 differentially expressed proteins, mainly involving T3SS-associated proteins (VscC and VopB), T6SS-associated proteins (Hcp, VgrG, VasG, ImpC, and ImpL), c-di-GMP-associated proteins (GGDEF and EAL-domain-containing proteins), biofilm-associated proteins (VpsT-like, GlnA, and GST), motility- and chemotaxis-associated proteins (FliC and MCP), and putative virulence-associated proteins (PAAR-like-AHH, TcpQ, and VesC). Together, these phenotypic and proteomic findings suggest that\n                    <jats:italic>vscW<\/jats:italic>\n                    deletion is associated with alterations in T3SS1 and other virulence-related functional networks. Collectively, our findings support a contribution of VscW to virulence-associated phenotypes and pathogenicity in\n                    <jats:italic>V. parahaemolyticus<\/jats:italic>\n                    and provide a basis for further investigation of the underlying regulatory mechanisms.\n                  <\/jats:p>","DOI":"10.3389\/fcimb.2026.1961181","type":"journal-article","created":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T04:27:17Z","timestamp":1790310437000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["T3SS1 accessory protein gene vscW alters motility, biofilm formation, outer membrane homeostasis, and virulence in Vibrio parahaemolyticus"],"prefix":"10.3389","volume":"16","author":[{"given":"Yuanhao","family":"Zhang","sequence":"first","affiliation":[{"name":"State Key Laboratory of Mariculture Biobreeding and Sustainable Goods, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences","place":["Qingdao, Shandong, China"]},{"name":"College of Fisheries, Huazhong Agricultural University","place":["Wuhan, Hubei, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chunyuan","family":"Wang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Mariculture Biobreeding and Sustainable Goods, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences","place":["Qingdao, Shandong, China"]},{"name":"Laboratory of Marine Fishery Science and Food Yield Process, Qingdao Marine Science and Technology Center","place":["Qingdao, Shandong, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yingeng","family":"Wang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Mariculture Biobreeding and Sustainable Goods, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences","place":["Qingdao, Shandong, China"]},{"name":"Laboratory of Marine Fishery Science and Food Yield Process, Qingdao Marine Science and Technology Center","place":["Qingdao, Shandong, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zheng","family":"Zhang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Mariculture Biobreeding and Sustainable Goods, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences","place":["Qingdao, Shandong, China"]},{"name":"Laboratory of Marine Fishery Science and Food Yield Process, Qingdao Marine Science and Technology Center","place":["Qingdao, Shandong, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yong-An","family":"Zhang","sequence":"additional","affiliation":[{"name":"College of Fisheries, Huazhong Agricultural University","place":["Wuhan, Hubei, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yongxiang","family":"Yu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Mariculture Biobreeding and Sustainable Goods, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences","place":["Qingdao, Shandong, China"]},{"name":"Laboratory of Marine Fishery Science and Food Yield Process, Qingdao Marine Science and Technology Center","place":["Qingdao, Shandong, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhiqi","family":"Zhang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Mariculture Biobreeding and Sustainable Goods, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences","place":["Qingdao, Shandong, China"]},{"name":"Laboratory of Marine Fishery Science and Food Yield Process, Qingdao Marine Science and Technology Center","place":["Qingdao, Shandong, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaojun","family":"Rong","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Mariculture Biobreeding and Sustainable Goods, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences","place":["Qingdao, Shandong, China"]},{"name":"Laboratory of Marine Fishery Science and Food Yield Process, Qingdao Marine Science and Technology Center","place":["Qingdao, Shandong, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinzhang","family":"Ji","sequence":"additional","affiliation":[{"name":"Hebei Yuanzhang Aquaculture Co., Ltd.","place":["Cangzhou, Hebei, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2026,9,25]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"7707","DOI":"10.1073\/pnas.1700286114","article-title":"RsmA and AmrZ orchestrate the assembly of all three type VI secretion systems in Pseudomonas aeruginosa","volume":"114","author":"Allsopp","year":"2017","journal-title":"Proc. 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