{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T23:57:14Z","timestamp":1788479834821,"version":"build-2803163510"},"reference-count":34,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2022,1,3]],"date-time":"2022-01-03T00:00:00Z","timestamp":1641168000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Cardiovasc. Med."],"abstract":"<jats:p>\n                    Cardiovascular dysfunction in children born after\n                    <jats:italic>in vitro<\/jats:italic>\n                    fertilization (IVF) has been of great concern, the potential molecular mechanisms for such long-term outcomes are still unknown. Here, we found that systolic blood pressure was a little higher in IVF born offspring at 2 years old compared to those born after being naturally conceived. Besides, the expression level of maternally expressed gene 3 (\n                    <jats:italic>MEG3<\/jats:italic>\n                    ) was higher in human umbilical vein endothelial cells (HUVECs) from IVF offspring than that in spontaneously born offspring. Pearson correlation test showed that\n                    <jats:italic>MEG3<\/jats:italic>\n                    relative expression is significantly related to the children's blood pressure (Coefficient = 0.429,\n                    <jats:italic>P<\/jats:italic>\n                    = 0.0262). Furthermore, we found decreased expression of endothelial nitric oxide synthase (\n                    <jats:italic>eNOS<\/jats:italic>\n                    ) and vascular endothelial growth factor\n                    <jats:italic>(VEGF)<\/jats:italic>\n                    along with elevated expression of endothelial-1(\n                    <jats:italic>ET1<\/jats:italic>\n                    ) in HUVECs from IVF offspring, accompanied by lower secretion of nitrite,\n                    <jats:italic>VEGF<\/jats:italic>\n                    , and higher secretion of\n                    <jats:italic>ET1<\/jats:italic>\n                    in the umbilical cord serum of IVF offspring. Correlation analysis showed MEG3 expression highly correlated with ET1 and Nitrate concentration. With pyrosequencing technology, we found that elevated expression of\n                    <jats:italic>MEG3<\/jats:italic>\n                    was the result of hypomethylation of the\n                    <jats:italic>MEG3<\/jats:italic>\n                    promoter. Therefore, our results provide a potential mechanism addressing the high-risk of hypertension in IVF offspring via MEG3 epigenetic regulation.\n                  <\/jats:p>","DOI":"10.3389\/fcvm.2021.717729","type":"journal-article","created":{"date-parts":[[2022,1,3]],"date-time":"2022-01-03T01:28:52Z","timestamp":1641173332000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":9,"title":["Elevated Expression of lncRNA MEG3 Induces Endothelial Dysfunction on HUVECs of IVF Born Offspring via Epigenetic Regulation"],"prefix":"10.3389","volume":"8","author":[{"given":"Ying","family":"Jiang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hong","family":"Zhu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hong","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi-Chen","family":"Yu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ye-Tao","family":"Xu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fang","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sai-Nan","family":"He","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthew","family":"Sagnelli","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi-Min","family":"Zhu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qiong","family":"Luo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2022,1,3]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"366","DOI":"10.1016\/S0140-6736(78)92957-4","article-title":"Birth 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