{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T15:53:43Z","timestamp":1753890823690,"version":"3.41.2"},"reference-count":53,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2022,11,16]],"date-time":"2022-11-16T00:00:00Z","timestamp":1668556800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003196","name":"Ministero della Salute","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003196","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003196","name":"Ministero della Salute","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003196","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Cardiovasc. Med."],"abstract":"<jats:p>The constantly increasing incidence of coronary artery disease worldwide makes necessary to set advanced therapies and tools such as tissue engineered vessel grafts (TEVGs) to surpass the autologous grafts [(i.e., mammary and internal thoracic arteries, saphenous vein (SV)] currently employed in coronary artery and vascular surgery. To this aim, <jats:italic>in vitro<\/jats:italic> cellularization of artificial tubular scaffolds still holds a good potential to overcome the unresolved problem of vessel conduits availability and the issues resulting from thrombosis, intima hyperplasia and matrix remodeling, occurring in autologous grafts especially with small caliber (&amp;lt;6 mm). The employment of silk-based tubular scaffolds has been proposed as a promising approach to engineer small caliber cellularized vascular constructs. The advantage of the silk material is the excellent manufacturability and the easiness of fiber deposition, mechanical properties, low immunogenicity and the extremely high <jats:italic>in vivo<\/jats:italic> biocompatibility. In the present work, we propose a method to optimize coverage of the luminal surface of silk electrospun tubular scaffold with endothelial cells. Our strategy is based on seeding endothelial cells (ECs) on the luminal surface of the scaffolds using a low-speed rolling. We show that this procedure allows the formation of a nearly complete EC monolayer suitable for flow-dependent studies and vascular maturation, as a step toward derivation of complete vascular constructs for transplantation and disease modeling.<\/jats:p>","DOI":"10.3389\/fcvm.2022.1013183","type":"journal-article","created":{"date-parts":[[2022,11,16]],"date-time":"2022-11-16T04:22:24Z","timestamp":1668572544000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":4,"title":["Luminal endothelialization of small caliber silk tubular graft for vascular constructs engineering"],"prefix":"10.3389","volume":"9","author":[{"given":"Stefano","family":"Rizzi","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sara","family":"Mantero","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Federica","family":"Boschetti","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maurizio","family":"Pesce","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1965","published-online":{"date-parts":[[2022,11,16]]},"reference":[{"key":"B1","doi-asserted-by":"crossref","first-page":"12","DOI":"10.1093\/eurheartj\/ehz859","article-title":"European society of cardiology: cardiovascular disease statistics 2019.","volume":"41","author":"Timmis","year":"2020","journal-title":"Eur Heart J."},{"key":"B2","doi-asserted-by":"publisher","first-page":"e139","DOI":"10.1161\/CIR.0000000000000746","article-title":"heart disease and stroke statistics-2020 update: a report from the American heart association.","volume":"141","author":"Virani","year":"2020","journal-title":"Circulation."},{"key":"B3","doi-asserted-by":"publisher","first-page":"23","DOI":"10.1007\/s12928-020-00734-7","article-title":"Mechanisms of drug-eluting stent restenosis.","volume":"36","author":"Aoki","year":"2021","journal-title":"Cardiovasc Interv Ther."},{"key":"B4","doi-asserted-by":"publisher","first-page":"155","DOI":"10.1038\/s41569-019-0249-3","article-title":"Saphenous vein grafts in contemporary coronary artery bypass graft surgery.","volume":"17","author":"Caliskan","year":"2020","journal-title":"Nat Rev Cardiol."},{"key":"B5","doi-asserted-by":"publisher","first-page":"e4","DOI":"10.1161\/CIR.0000000000001061","article-title":"2021 ACC\/AHA\/SCAI guideline for coronary artery revascularization: executive summary: a report of the American college of cardiology\/American heart association joint committee on clinical practice guidelines.","volume":"145","author":"Lawton","year":"2022","journal-title":"Circulation."},{"key":"B6","doi-asserted-by":"publisher","first-page":"109","DOI":"10.1161\/CIRCRESAHA.121.319984","article-title":"Bioengineering human tissues and the future of vascular replacement.","volume":"131","author":"Naegeli","year":"2022","journal-title":"Circ Res."},{"key":"B7","doi-asserted-by":"publisher","first-page":"1445","DOI":"10.1161\/CIRCULATIONAHA.113.008193","article-title":"Saphenous vein graft failure after coronary artery bypass surgery: insights from PREVENT IV.","volume":"130","author":"Hess","year":"2014","journal-title":"Circulation."},{"key":"B8","doi-asserted-by":"publisher","first-page":"S2245","DOI":"10.1016\/S0003-4975(01)03272-6","article-title":"The coronary artery bypass conduit: I. 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