{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,29]],"date-time":"2025-10-29T03:52:35Z","timestamp":1761709955980,"version":"3.41.2"},"reference-count":21,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2022,5,31]],"date-time":"2022-05-31T00:00:00Z","timestamp":1653955200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100002855","name":"Ministry of Science and Technology of the People's Republic of China","doi-asserted-by":"publisher","award":["2019YFA0802600"],"award-info":[{"award-number":["2019YFA0802600"]}],"id":[{"id":"10.13039\/501100002855","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Cardiovasc. Med."],"abstract":"<jats:sec><jats:title>Objective<\/jats:title><jats:p>Calcium voltage-gated channel subunit alpha1 C (CACNA1C) plays a critical role in many vascular physiological and pathological processes. Determining its tissue-specific expression pattern and clarifying the underlying molecular mechanisms are necessary and meaningful.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>We selected several representative vessels from normal male Sprague-Dawley rats. Vessel tissue or primary vascular smooth muscle cells were isolated for vascular function, electrophysiology, gene expression and promoter methylation studies.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>We found CACNA1C had tissue-specific expressions in vessels. The specific manifestations were as follows: CACNA1C expression was highest in thoracic aorta, second lowest in middle cerebral and pulmonary artery, and lowest in mesenteric artery. Excitingly, an opposing trend was observed between CACNA1C expression and its promoter methylation.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>This study was the first report to indicate that DNA methylation could be involved in regulating CACNA1C tissue-specific expressions and vasoconstriction function in vascular system. This study not only provided more information for further understanding the physiological characteristics of vascular CACNA1C expressions, also strengthened the idea that DNA methylation plays important roles in regulating vascular smooth muscle cells function and the consequent occurrence of vascular diseases.<\/jats:p><\/jats:sec>","DOI":"10.3389\/fcvm.2022.872977","type":"journal-article","created":{"date-parts":[[2022,6,1]],"date-time":"2022-06-01T09:42:51Z","timestamp":1654076571000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":3,"title":["Unveiling the Role of DNA Methylation in Vascular CACNA1C Tissue\u2013Specific Expression"],"prefix":"10.3389","volume":"9","author":[{"given":"Meng","family":"Zhao","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ting","family":"Xu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jiahui","family":"Lei","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bingyu","family":"Ji","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qinqin","family":"Gao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1965","published-online":{"date-parts":[[2022,5,31]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"5361","DOI":"10.1113\/JP280950","article-title":"Vascular calcium signalling and ageing","volume":"599","author":"Harraz","year":"2021","journal-title":"J Physiol."},{"key":"B2","doi-asserted-by":"publisher","first-page":"237","DOI":"10.1093\/cvr\/cvn345","article-title":"Vascular smooth muscle cells sense calcium: a new paradigm in vascular calcification","volume":"81","author":"Touyz","year":"2009","journal-title":"Cardiovasc Res."},{"key":"B3","doi-asserted-by":"publisher","first-page":"1271","DOI":"10.1007\/s00424-018-2151-y","article-title":"Calcium- and voltage-gated BK channels in vascular smooth muscle","volume":"470","author":"Dopico","year":"2018","journal-title":"Pflugers Arch."},{"key":"B4","doi-asserted-by":"publisher","first-page":"eabc6438","DOI":"10.1126\/scisignal.abc6438","article-title":"Tissue-specific adrenergic regulation of the L-type Ca2+ channel CaV1","volume":"13","author":"Man","year":"2020","journal-title":"Sci Signal"},{"key":"B5","doi-asserted-by":"publisher","first-page":"463527","DOI":"10.1155\/2013\/463527","article-title":"Cav1","volume":"2013","author":"Soldatov","year":"2013","journal-title":"ISRN Biochem."},{"key":"B6","doi-asserted-by":"publisher","first-page":"e019922","DOI":"10.1161\/JAHA.120.019922","article-title":"antenatal hypoxia affects pulmonary artery contractile functions via downregulating L-type Ca2+ channels subunit alpha1 C in adult male offspring","volume":"10","author":"Li","year":"2021","journal-title":"J Am Heart Assoc."},{"key":"B7","doi-asserted-by":"publisher","first-page":"15058","DOI":"10.1074\/jbc.M110.182816","article-title":"Jaggar JH. 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