{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,2]],"date-time":"2026-08-02T08:10:26Z","timestamp":1785658226297,"version":"3.56.0"},"reference-count":33,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2023,6,12]],"date-time":"2023-06-12T00:00:00Z","timestamp":1686528000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Cardiovasc. Med."],"abstract":"<jats:sec><jats:title>Objective<\/jats:title><jats:p>Type IV collagen (Col-IV) is a prospective biomarker for diagnosing and treating of unstable thoracic aortic aneurysm and dissection (TAAD). This study aims to evaluate the feasibility of <jats:sup>68<\/jats:sup>Ga-labeled WVP peptide (<jats:sup>68<\/jats:sup>Ga-DOTA-WVP) as a novel Col-IV-targeted probe for TAAD biological diagnosis using PET\/CT.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>WVP peptide was modified with bifunctional chelator DOTA for <jats:sup>68<\/jats:sup>Ga radiolabeling. Immunohistochemical staining was used to evaluate the expression and location of Col-IV and elastin in aortas treated with 3-aminopropionitrile fumarate (BAPN) at different time points (0, 2, and 4 weeks). The imaging performance of <jats:sup>68<\/jats:sup>Ga-DOTA-WVP was investigated using Micro-PET\/CT in a BAPN-induced TAAD mouse model. The relationship between <jats:sup>68<\/jats:sup>Ga-DOTA-WVP uptake in aortic lesions and the serum levels of TAAD-related biomarkers including D-dimer, C-reactive protein (CRP), and serum soluble suppression of tumorigenicity\u22122 (sST2) was also analyzed.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p><jats:sup>68<\/jats:sup>Ga-DOTA-WVP was readily prepared with high radiochemical purity and stability <jats:italic>in vitro<\/jats:italic>. <jats:sup>68<\/jats:sup>Ga-DOTA-WVP Micro-PET\/CT could detect Col-IV exposure of unstable aneurysms and early dissection in BAPN-induced TAAD mice, but little <jats:sup>68<\/jats:sup>Ga-DOTA-WVP uptake was shown in the control group at each imaging time point. The differences of Col-IV expression and distribution of <jats:sup>68<\/jats:sup>Ga-DOTA-WVP both in TAAD and control groups further verified the imaging efficiency of <jats:sup>68<\/jats:sup>Ga-DOTA-WVP PET\/CT. Additionally, a higher sST2 level was found in the imaging positive (<jats:italic>n<\/jats:italic>\u2009=\u200914) than the negative (<jats:italic>n<\/jats:italic>\u2009=\u20098) group (9.60\u2009\u00b1\u20091.14 vs. 8.44\u2009\u00b1\u20090.52, <jats:italic>P<\/jats:italic>\u2009=\u20090.014).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p><jats:sup>68<\/jats:sup>Ga-DOTA-WVP could trace the exposure and abnormal deposition of Col-IV in enlarged and early injured aortas, showing a potential for biological diagnosis, whole-body screening, and progression monitoring of TAAD.<\/jats:p><\/jats:sec>","DOI":"10.3389\/fcvm.2023.1048927","type":"journal-article","created":{"date-parts":[[2023,6,12]],"date-time":"2023-06-12T04:35:19Z","timestamp":1686544519000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":3,"title":["68Ga-labeled WVP peptide as a novel PET probe for molecular biological diagnosis of 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