{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,27]],"date-time":"2026-05-27T18:57:25Z","timestamp":1779908245871,"version":"3.53.1"},"reference-count":36,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2024,9,5]],"date-time":"2024-09-05T00:00:00Z","timestamp":1725494400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003392","name":"Fujian Provincial Natural Science Foundation","doi-asserted-by":"publisher","award":["2022J01749"],"award-info":[{"award-number":["2022J01749"]}],"id":[{"id":"10.13039\/501100003392","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Cardiovasc. Med."],"abstract":"<jats:sec><jats:title>Objective<\/jats:title><jats:p>This study strives to the variation and significance of microRNA-21 (<jats:italic>miR-21<\/jats:italic>) in children with congenital heart disease (CHD)-related pulmonary artery hypertension (PAH).<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Children with CHD (<jats:italic>n<\/jats:italic>\u2009=\u2009179) were selected as subjects, including 101 children without PAH and 78 children with PAH. All children underwent general data collection, laboratory examination, echocardiography and cardiac catheterization. After detection of serum <jats:italic>miR-21<\/jats:italic> expression, the predictive value and the impacts of serum <jats:italic>miR-21<\/jats:italic> for PAH and postoperative critical illness were analyzed.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Serum creatine kinase isoenzyme (CK-MB), B-type natriuretic peptide (BNP) and <jats:italic>miR-21<\/jats:italic> were elevated, but ejection fraction (EF) and cardiac index (CI) were decreased in the CHD-PAH group. Serum <jats:italic>miR-21<\/jats:italic> assisted in predicting PAH in CHD children, with the area under curve (AUC) of 0.801 (95% CI of 0.735\u223c0.857), a cut-off value of 2.56, sensitivity of 73.08, and specificity of 72.28%. Serum <jats:italic>miR-21<\/jats:italic> in children with CHD-PAH was correlated with clinicopathological indicators such as systolic pulmonary artery pressure, mean pulmonary arterial pressure, BNP and CI. Serum <jats:italic>miR-21<\/jats:italic> helped predict the development of postoperative critical illness in children with CHD-PAH, with an AUC of 0.859 (95% CI: 0.762\u20130.927, cut-off value: 4.55, sensitivity: 69.57%, specificity: 92.73%). Increased serum <jats:italic>miR-21<\/jats:italic> was an independent risk factor of postoperative critical illness in children with CHD-PAH.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Serum <jats:italic>miR-21<\/jats:italic> was upregulated in children with CHD-PAH, which may serve as a predictive biomarker for the onset of PAH and postoperative critical illness in CHD children.<\/jats:p><\/jats:sec>","DOI":"10.3389\/fcvm.2024.1424679","type":"journal-article","created":{"date-parts":[[2024,9,5]],"date-time":"2024-09-05T04:56:09Z","timestamp":1725512169000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":4,"title":["Variation and significance of serum microRNA-21 level in pediatric pulmonary artery hypertension associated with congenital heart disease"],"prefix":"10.3389","volume":"11","author":[{"given":"Yanming","family":"Shen","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dongshan","family":"Liao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenlin","family":"Shangguan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liangwan","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2024,9,5]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"879","DOI":"10.3390\/biom9120879","article-title":"Genetics of congenital heart disease","volume":"9","author":"Williams","year":"2019","journal-title":"Biomolecules"},{"key":"B2","doi-asserted-by":"publisher","first-page":"341","DOI":"10.2174\/1570161120666220811150853","article-title":"Targeted 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