{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,11]],"date-time":"2026-06-11T06:01:34Z","timestamp":1781157694637,"version":"3.54.1"},"reference-count":23,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,6,11]],"date-time":"2026-06-11T00:00:00Z","timestamp":1781136000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Cardiovasc. Med."],"abstract":"<jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Acute myocardial infarction (AMI) is a leading cause of death worldwide, occurring earlier and more severely in South Asian populations. Circulating microRNAs (miRNAs) are stable, non-invasive biomarkers with emerging diagnostic and prognostic potential, but population-specific data from South India remain limited.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      In this prospective cohort, 216 participants were enrolled: 45 AMI patients, 71 stable coronary artery disease (CAD) patients, and 100 healthy controls. Plasma samples were collected within 24\u2005h of symptom onset in AMI cases. Expression of prioritized circulating miRNAs (\n                      <jats:italic>n<\/jats:italic>\n                      \u2009=\u200920) was quantified using qRT-PCR SYBR green chemistry using specific primers. Statistical analyses included hierarchical and k-means clustering, principal component analysis (PCA), pathway enrichment, and receiver operating characteristic (ROC) curve analyses.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results and conclusion<\/jats:title>\n                    <jats:p>\n                      MI patients showed significant upregulation of let-7b, let-7c-5p, miR-24-1, miR-342-3p, and miR-362-3p, and downregulation of miR-4485-3p, miR-494-3p, miR-939, and miR-4505 (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&amp;lt;\u20090.05). Cluster and PCA analyses revealed distinct segregation of AMI cases, while CAD exhibited intermediate molecular profiles. Pathway enrichment implicated ECM\u2013receptor interaction, platelet activation, and PI3K\/AKT signaling. ROC analysis demonstrated excellent discriminatory performance, with miR-4485-3p achieving an AUC of 1.000. There were no significant differences in the expression pattern of miR-3195, miR-6780b-5p between CAD and AMI patients. These findings highlight circulating miRNAs as promising biomarkers for AMI, reflecting key molecular processes of inflammation, apoptosis, and endothelial dysfunction.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.3389\/fcvm.2026.1730999","type":"journal-article","created":{"date-parts":[[2026,6,11]],"date-time":"2026-06-11T05:49:02Z","timestamp":1781156942000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["Utility of miRNA biomarkers in patients with acute myocardial infarction (UNIAMI)\u2014results from a prospective pilot study"],"prefix":"10.3389","volume":"13","author":[{"given":"Chakradhara Rao S.","family":"Uppugunduri","sequence":"first","affiliation":[{"name":"Department of Medical Oncology, Jawaharlal Institute of Postgraduate Medical Education and Research","place":["Puducherry, India"]},{"name":"Department of Pediatrics, Gynecology and Obstetrics, CANSEARCH research platform of Pediatric Oncology and Hematology of University of Geneva, Geneva","place":["Switzerland"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Koustav","family":"Sarkar","sequence":"additional","affiliation":[{"name":"Department of Biotechnology, School of Bioengineering, College of Engineering and Technology, SRM Institute of Science and Technology","place":["Chennai, India"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Oishi","family":"Mukherjee","sequence":"additional","affiliation":[{"name":"Centre for Clinical Pharmacology, SRM Medical College Hospital & Research Centre","place":["Kattankulathur, India"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kaviya","family":"Manoharan","sequence":"additional","affiliation":[{"name":"Centre for Clinical Pharmacology, SRM Medical College Hospital & Research Centre","place":["Kattankulathur, India"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Iyshwarya","family":"B. 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