{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,15]],"date-time":"2026-04-15T06:46:07Z","timestamp":1776235567574,"version":"3.50.1"},"reference-count":10,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,4,15]],"date-time":"2026-04-15T00:00:00Z","timestamp":1776211200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Cardiovasc. Med."],"abstract":"<jats:p>\n                    The safe ischemic window for static cold storage in heart transplantation is around 4\u20136\u2005h, limiting donor retrieval distance and contributing to organ discard. We aim to assess whether a porcine heart preserved for 24\u2005h using a novel ECMO-based ESHP system can maintain function after orthotopic transplantation. Five-orthotopic allogeneic heart transplantation were preserved for 24\u2005h on a custom ECMO-based ESHP circuit at 37\u2005\u00b0C, with coronary perfusion pressure 60\u201370\u2005mmHg, and metabolic\/hormonal supplementation. Hemodynamic, metabolic, and oxygenation parameters were measured using pulmonary arterial thermodilution via a Swan-Ganz catheter. Recipients were monitored for 4\u2005h post-transplantation: cardiac output increased from 5.94\u2009\u00b1\u20090.16\u2005L\/min (baseline) to 6.78\u2009\u00b1\u20090.12\u2005L\/min (immediately post-transplant) (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009=\u20090.006) and 7.06\u2009\u00b1\u20090.16\u2005L\/min at 4\u2005h following transplantation (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009=\u20090.017). Despite significant reductions in hemoglobin and arterial oxygen content, oxygen delivery remained adequate, oxygen consumption increased significantly at 4\u2005h following transplantation to 172.88\u2009\u00b1\u20097.60\u2005mL\/min\/m\u00b2 (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009=\u20090.0322 vs. donor) and lactate levels stayed low. No evidence of acute graft dysfunction was observed. Normothermic preservation of the heart with our institutional ESHP protocol for 24\u2005h maintained graft viability and function in a porcine transplantation model.\n                  <\/jats:p>","DOI":"10.3389\/fcvm.2026.1805585","type":"journal-article","created":{"date-parts":[[2026,4,15]],"date-time":"2026-04-15T05:44:41Z","timestamp":1776231881000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["Prolonged ex-situ heart perfusion preserves cardiac output following orthotopic transplantation in a porcine model"],"prefix":"10.3389","volume":"13","author":[{"given":"Zhuldyz","family":"Nurmykhametova","sequence":"first","affiliation":[{"name":"Department of Perfusiology and Assisted Circulation Laboratory, University Medical Center Corporate Fund","place":["Astana, Kazakhstan"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rymbay","family":"Kaliyev","sequence":"additional","affiliation":[{"name":"Department of Perfusiology and Assisted Circulation Laboratory, University Medical Center Corporate Fund","place":["Astana, Kazakhstan"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Timur","family":"Lesbekov","sequence":"additional","affiliation":[{"name":"Department of Adult Cardiac Surgery, University Medical Center Corporate Fund","place":["Astana, Kazakhstan"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Linar","family":"Faizov","sequence":"additional","affiliation":[{"name":"Department of Perfusiology and Assisted Circulation Laboratory, University Medical Center Corporate Fund","place":["Astana, Kazakhstan"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Assel","family":"Medressova","sequence":"additional","affiliation":[{"name":"Department of Adult Cardiac Surgery, University Medical Center Corporate Fund","place":["Astana, Kazakhstan"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aiym","family":"Turarova","sequence":"additional","affiliation":[{"name":"Department of Anesthesiology, University Medical Center Corporate Fund , , .","place":["Astana, Kazakhstan"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yerik","family":"Zuparov","sequence":"additional","affiliation":[{"name":"Department of Anesthesiology, University Medical Center Corporate Fund , , .","place":["Astana, Kazakhstan"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuriy","family":"Pya","sequence":"additional","affiliation":[{"name":"Department of Anesthesiology, University Medical Center Corporate Fund , , .","place":["Astana, Kazakhstan"]}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1965","published-online":{"date-parts":[[2026,4,15]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"16693","DOI":"10.3390\/ijms242316693","article-title":"Donor heart preservation: current knowledge and the new era of machine perfusion","volume":"24","author":"Kounatidis","year":"2023","journal-title":"Int J Mol Sci"},{"key":"B2","doi-asserted-by":"publisher","first-page":"69","DOI":"10.1016\/j.healun.2020.10.004","article-title":"Ex situ heart perfusion: the past, the present, and the future","volume":"40","author":"Wang","year":"2021","journal-title":"J Heart Lung Transplant"},{"key":"B3","doi-asserted-by":"publisher","first-page":"499","DOI":"10.1016\/j.athoracsur.2019.01.076","article-title":"Myocardial functional decline during prolonged ex situ heart perfusion","volume":"108","author":"Hatami","year":"2019","journal-title":"Ann Thorac 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Med"},{"key":"B10","doi-asserted-by":"publisher","first-page":"e1701","DOI":"10.1097\/TXD.0000000000001701","article-title":"Successful resuscitation of porcine hearts after 12 and 24\u2005h of static cold storage with normothermic ex situ perfusion","volume":"10","author":"Johnson","year":"2024","journal-title":"Transplant Direct"}],"container-title":["Frontiers in Cardiovascular Medicine"],"original-title":[],"link":[{"URL":"https:\/\/www.frontiersin.org\/articles\/10.3389\/fcvm.2026.1805585\/full","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,15]],"date-time":"2026-04-15T05:44:41Z","timestamp":1776231881000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.frontiersin.org\/articles\/10.3389\/fcvm.2026.1805585\/full"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,4,15]]},"references-count":10,"alternative-id":["10.3389\/fcvm.2026.1805585"],"URL":"https:\/\/doi.org\/10.3389\/fcvm.2026.1805585","relation":{},"ISSN":["2297-055X"],"issn-type":[{"value":"2297-055X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,4,15]]},"article-number":"1805585"}}