{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,20]],"date-time":"2026-07-20T06:03:44Z","timestamp":1784527424805,"version":"3.55.0"},"reference-count":41,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,7,20]],"date-time":"2026-07-20T00:00:00Z","timestamp":1784505600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100014718","name":"Innovative Research Group Project of the National Natural Science Foundation of China","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100014718","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Cardiovasc. Med."],"abstract":"<jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>To investigate the protective effects of the active parts of Yangxin Tongmai Formula (apr-YTF) against myocardial ischemia-reperfusion injury (MIRI) in rats from the perspective of spatial metabolic remodeling.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>A rat model of MIRI with blood stasis syndrome was established. Rats were randomized into Control, Model, Vehicle, and apr-YTF groups. apr-YTF was administered by gavage for 14 days. Cardiac function and myocardial injury were assessed by echocardiography, serum myocardial enzyme assays, triphenyltetrazolium chloride (TTC) staining, and hematoxylin and eosin (HE) staining. Matrix-assisted laser desorption\/ionization mass spectrometry imaging (MALDI-MSI) was used to analyze the spatial distribution patterns and metabolic profiles of differential metabolites in myocardial tissue.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>apr-YTF significantly improved left ventricular ejection fraction (LVEF) and left ventricular fractional shortening (LVFS), reduced serum creatine kinase (CK), creatine kinase-MB isoenzyme (CK-MB), lactate dehydrogenase (LDH), and lactate dehydrogenase 1 (LDH1) levels, decreased infarct size, and alleviated myocardial fibrosis and inflammatory infiltration. MALDI-MSI identified 1,282 metabolites, including 702 MIRI model-associated differential metabolic features, mainly including amino acids, nucleotides, and glycerophospholipids. The MIRI group exhibited extensive metabolic remodeling involving lipid, amino acid, nucleotide metabolism, and cofactor biosynthesis pathways. After apr-YTF intervention, 17 reversed differential metabolic features showed recovery trends.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>The active parts of Yangxin Tongmai Formula ameliorated myocardial injury and were associated with partial correction of spatial metabolic remodeling in rats with MIRI.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.3389\/fcvm.2026.1857561","type":"journal-article","created":{"date-parts":[[2026,7,20]],"date-time":"2026-07-20T05:35:40Z","timestamp":1784525740000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["Effects of the active parts of Yangxin Tongmai Formula on myocardial injury and spatial metabolic remodeling in rats with myocardial ischemia-reperfusion injury"],"prefix":"10.3389","volume":"13","author":[{"given":"Di","family":"Ye","sequence":"first","affiliation":[{"name":"Graduate School, Guangxi University of Chinese Medicine","place":["Nanning, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi","family":"Wang","sequence":"additional","affiliation":[{"name":"Graduate School, Guangxi University of Chinese Medicine","place":["Nanning, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ziyuan","family":"Zhao","sequence":"additional","affiliation":[{"name":"Graduate School, Guangxi University of Chinese Medicine","place":["Nanning, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Caiyue","family":"Lin","sequence":"additional","affiliation":[{"name":"Graduate School, Guangxi University of Chinese Medicine","place":["Nanning, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenhua","family":"Xu","sequence":"additional","affiliation":[{"name":"Graduate School, Guangxi University of Chinese Medicine","place":["Nanning, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jinghui","family":"Zheng","sequence":"additional","affiliation":[{"name":"Academic Affairs Office, Guangxi University of Chinese Medicine","place":["Nanning, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2026,7,20]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"2135","DOI":"10.1016\/j.jacc.2024.11.009","article-title":"2025 ACC\/AHA\/ACEP\/NAEMSP\/SCAI guideline for the management of patients with acute coronary syndromes: a report of the American college of cardiology\/American heart association joint committee on clinical practice guidelines","volume":"85","author":"Rao","year":"2025","journal-title":"J Am 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