{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T09:35:59Z","timestamp":1790328959944,"version":"4.1.0"},"reference-count":56,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2021,1,25]],"date-time":"2021-01-25T00:00:00Z","timestamp":1611532800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Endocrinol."],"abstract":"<jats:sec><jats:title>Context<\/jats:title><jats:p>The widespread distribution of the Vitamin D (VitD) receptor in reproductive tissues suggests an important role for VitD in human reproduction. The assessment of patient\u00b4s VitD is based on the 25-hydroxyvitamin D (25(OH)D) metabolite measurement. However, most of the circulating 25(OH)D is bound to either VitD-binding protein (VDBP) (88%) or albumin (12%) and less than 1% circulates free.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objective<\/jats:title><jats:p>To determine a possible correlation between VitD levels in serum (S) and follicular fluid (FF) and blastocyst ploidy status in patients undergoing infertility treatment.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>A prospective observational study was performed including couples planned for preimplantation genetic testing for aneuploidies (PGT-A) from ART Fertility Clinics. Patients were classified according to their 25(OH)D-Serum levels: VitD deficient group &amp;lt;20 ng\/ml and insufficient\/replete \u226520 ng\/ml defined as VitD non-deficient group.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Serum samples and 226 FF from individual follicles were collected for 25(OH)D, bioavailable 25(OH)D, free 25(OH)D, and % free 25(OH)D measurement. 25(OH)D-Serum in VitD deficient and non-deficient were 13.2\u00b14.0 ng\/ml vs 32.3\u00b19.2 ng\/ml; p&amp;lt;0.001. FF from 40 and 74 biopsied blastocysts was analysed of which 52.5 and 60.8% were euploid (p = 0.428), respectively. In VitD deficient patients, mean 25(OH)D-FF, bioavailable 25(OH)D-FF, and free 25(OH)D-FF were higher in euploid vs aneuploid blastocysts (18.3\u00b16.3 ng\/ml vs 13.9\u00b14.8 ng\/ml; p = 0.040; 1.5\u00b10.5 ng\/ml vs 1.1\u00b10.4 ng\/ml; p = 0.015; 0.005\u00b10.002 ng\/ml vs 0.003\u00b10.001 ng\/ml; p = 0.023, respectively), whilst no differences were found in VitD non-deficient patients (37.9\u00b112.3 ng\/ml vs 40.6\u00b113.7 ng\/ml; p = 0.380; 3.1\u00b11.1 ng\/ml vs 3.3\u00b11.2 ng\/ml; p = 0.323; 0.01\u00b10.003 ng\/ml vs 0.01\u00b10.004 ng\/ml; p = 0.319, respectively).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>VitD non-deficient patients have a significantly higher probability of obtaining a euploid blastocyst compared to VitD deficient patients (OR:33.36, p = 0.002).<\/jats:p><\/jats:sec>","DOI":"10.3389\/fendo.2020.609524","type":"journal-article","created":{"date-parts":[[2021,1,25]],"date-time":"2021-01-25T09:39:45Z","timestamp":1611567585000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":14,"title":["Vitamin D in Follicular Fluid Correlates With the Euploid Status of Blastocysts in a Vitamin D Deficient Population"],"prefix":"10.3389","volume":"11","author":[{"given":"Ana","family":"Arnanz","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Neelke","family":"De Munck","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ibrahim","family":"El 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