{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,24]],"date-time":"2026-08-24T13:57:10Z","timestamp":1787579830461,"version":"build-2736575974"},"reference-count":37,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2020,12,14]],"date-time":"2020-12-14T00:00:00Z","timestamp":1607904000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["18K15921, 18H02817G"],"award-info":[{"award-number":["18K15921, 18H02817G"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Endocrinol."],"abstract":"<jats:p>The generation of mature, functional, thyroid follicular cells from pluripotent stem cells would potentially provide a therapeutic benefit for patients with hypothyroidism, but<jats:italic>in vitro<\/jats:italic>differentiation remains difficult. We earlier reported the<jats:italic>in vivo<\/jats:italic>generation of lung organs<jats:italic>via<\/jats:italic>blastocyst complementation in fibroblast growth factor 10 (<jats:italic>Fgf10<\/jats:italic>), compound, heterozygous mutant (<jats:italic>Fgf10<\/jats:italic>Ex1<jats:sup>mut<\/jats:sup>\/Ex3<jats:sup>mut<\/jats:sup>) mice. Fgf10 also plays an essential role in thyroid development and branching morphogenesis, but any role thereof in thyroid organogenesis remains unclear. Here, we report that the thyroids of<jats:italic>Fgf10<\/jats:italic>Ex1<jats:sup>mut<\/jats:sup>\/Ex3<jats:sup>mut<\/jats:sup>mice exhibit severe hypoplasia, and we generate thyroid tissues from mouse embryonic stem cells (ESCs) in<jats:italic>Fgf10<\/jats:italic>Ex1<jats:sup>mut<\/jats:sup>\/Ex3<jats:sup>mut<\/jats:sup>mice<jats:italic>via<\/jats:italic>blastocyst complementation. The tissues were morphologically normal and physiologically functional. The thyroid follicular cells of<jats:italic>Fgf10<\/jats:italic>Ex1<jats:sup>mut<\/jats:sup>\/Ex3<jats:sup>mut<\/jats:sup>chimeric mice were derived largely from GFP-positive mouse ESCs although the recipient cells were mixed. Thyroid generation<jats:italic>in vivo via<\/jats:italic>blastocyst complementation will aid functional thyroid regeneration.<\/jats:p>","DOI":"10.3389\/fendo.2020.609697","type":"journal-article","created":{"date-parts":[[2020,12,14]],"date-time":"2020-12-14T05:45:48Z","timestamp":1607924748000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":17,"title":["Generation of Thyroid Tissues From Embryonic Stem Cells via Blastocyst Complementation In 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