{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T04:12:36Z","timestamp":1787026356470,"version":"build-2736575974"},"reference-count":55,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2021,4,2]],"date-time":"2021-04-02T00:00:00Z","timestamp":1617321600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Endocrinol."],"abstract":"<jats:p>\n                    Recurrent spontaneous abortion (RSA) remains a critical and challenging problem in reproduction. To discover novel biomarkers for RSA, ultra performance liquid chromatography\/tandem mass spectrometry (UPLC-MS\/MS) metabolomics approach was applied to detect RSA serum metabolic profiles and explore its possible pathogenesis and mechanism. The abortion rat model was established, and a metabolomics analysis was performed to evaluate the differentially expressed metabolites between the control and model groups. Immunohistochemistry (IHC), qRT-PCR, and Western blot further examined the expression of Arachidonic acid metabolism-related genes in uterus tissues. To identify arachidonic acid metabolism-related changes in RSA, ELISA\u2019s potential mechanisms were further confirmed in serum. Ninety-one metabolites were significantly different between the two groups, as indicated by a VIP \u22651, fold change \u22651. The metabolic pathways involving arachidonic acid metabolism pathway (\n                    <jats:italic>P<\/jats:italic>\n                    = 0.00044) are related to RSA. Verification by experimental showed that compared with the control rats, the expression of the COX-1, COX-2, PTGFR, and TBXA2R genes associated with the arachidonic acid metabolism pathway has significantly increased the uterus and serum of RSA rats (\n                    <jats:italic>P<\/jats:italic>\n                    &amp;lt; 0.05). Regulation of the arachidonic acid metabolism pathway might serve as a promising therapeutic strategy for relieving RSA women\u2019s symptoms.\n                  <\/jats:p>","DOI":"10.3389\/fendo.2021.652807","type":"journal-article","created":{"date-parts":[[2021,4,2]],"date-time":"2021-04-02T00:23:40Z","timestamp":1617323020000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":17,"title":["The Arachidonic Acid Metabolism Mechanism Based on UPLC-MS\/MS Metabolomics in Recurrent Spontaneous Abortion Rats"],"prefix":"10.3389","volume":"12","author":[{"given":"Meihe","family":"Li","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yang","family":"Haixia","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Minchao","family":"Kang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peng","family":"An","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xili","family":"Wu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huimin","family":"Dang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xin","family":"Xu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2021,4,2]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","DOI":"10.1089\/thy.2009.0323","article-title":"Thyroid function in women found to have early pregnancy loss","volume":"20","author":"De Vivo","year":"2010","journal-title":"Thyroid"},{"key":"B2","doi-asserted-by":"publisher","DOI":"10.1210\/jc.2010-0340","article-title":"Increased pregnancy loss rate in thyroid antibody negative women with TSH levels between 2.5 and 5.0 in the first trimester of pregnancy","volume":"95","author":"Negro","year":"2010","journal-title":"J Clin Endocrinol Metab"},{"key":"B3","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaut.2011.11.014","article-title":"Pathogenesis of infertility and recurrent pregnancy loss in thyroid autoimmunity","volume":"38","author":"Twig","year":"2012","journal-title":"J Autoimmun"},{"key":"B4","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMcp1005330","article-title":"Clinical practice. 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