{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,5]],"date-time":"2026-11-05T01:19:53Z","timestamp":1793841593200,"version":"4.6.1"},"reference-count":31,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2022,1,31]],"date-time":"2022-01-31T00:00:00Z","timestamp":1643587200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Endocrinol."],"abstract":"<jats:sec><jats:title>Objective<\/jats:title><jats:p>This study was aimed to investigate the effects of JiaYanKangTai (JYKT) on regulating interleukin-17 (IL-17) signaling in rats with autoimmune thyroiditis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Lewis rats were administrated with JYKT for eight weeks after a seven-week subcutaneous injection of thyroglobulin with adjuvant and feeding iodine water. Ultrasonography was performed and total volume of thyroid was calculated. The expressions of autoantibodies and hormones were detected. Morphological changes of thyroid were observed. Metabolomics profile and metabolic network analysis were conducted. IL-17 signaling was detected by polymerase chain reaction and immunohistochemistry separately.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>JYKT reduced the mean volumes of thyroid, decreased both levels of TPOAb and TGAb, and alleviated lymphocytic infiltration of the thyroid. Metabolic network analysis of metabolomics proved IL-17 signaling pathway as a critical pathway in JYKT administration for autoimmune thyroiditis. JYKT downregulated expressions of IL-17A, TRAF6, p-ERK1\/2 and TNF-\u03b1.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>JYKT alleviated inflammatory lesions of experimental autoimmune thyroiditis by regulating IL-17 signaling.<\/jats:p><\/jats:sec>","DOI":"10.3389\/fendo.2021.794568","type":"journal-article","created":{"date-parts":[[2022,1,31]],"date-time":"2022-01-31T14:53:10Z","timestamp":1643640790000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":6,"title":["A Chinese Patent Medicine JiaYanKangTai Alleviates Inflammatory Lesions of Experimental Autoimmune Thyroiditis by Regulating Interleukin-17 Signaling"],"prefix":"10.3389","volume":"12","author":[{"given":"Yajing","family":"Pan","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qiue","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chengfei","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lili","family":"Wu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lingling","family":"Qin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tonghua","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kaiwen","family":"Hu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2022,1,31]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","DOI":"10.1111\/cen.14304","article-title":"Prevalence of Latent and Overt Polyautoimmunity in Autoimmune Thyroid Disease: A Systematic Review and Meta-Analysis","volume":"93","author":"Botello","year":"2020","journal-title":"Clin Endocrinol (Oxf)"},{"key":"B2","doi-asserted-by":"publisher","DOI":"10.3390\/cells9030665","article-title":"Dysregulated Antibody, Natural Killer Cell and Immune Mediator Profiles in Autoimmune Thyroid Diseases","volume":"9","author":"Martin","year":"2020","journal-title":"Cells"},{"key":"B3","doi-asserted-by":"publisher","DOI":"10.1016\/j.beem.2019.101367","article-title":"Hashimotos\u2019 Thyroiditis: Epidemiology, Pathogenesis, Clinic and Therapy","volume":"33","author":"Ragusa","year":"2019","journal-title":"Best Pract Res Clin Endocrinol Metab"},{"key":"B4","doi-asserted-by":"publisher","DOI":"10.1016\/bs.vh.2017.07.001","article-title":"Thyroid Autoimmunity: An Interplay of Factors","volume":"106","author":"Merrill","year":"2018","journal-title":"Vitam Horm"},{"key":"B5","doi-asserted-by":"publisher","first-page":"34","DOI":"10.1017\/S0029665118001192","article-title":"Multiple Nutritional Factors and Thyroid Disease, With Particular Reference to Autoimmune Thyroid Disease","volume":"78","author":"Rayman","year":"2019","journal-title":"Proc Nutr Soc"},{"key":"B6","doi-asserted-by":"publisher","DOI":"10.1016\/j.beem.2020.101388","article-title":"Graves\u2019 Disease: Clinical Manifestations, Immune Pathogenesis (Cytokines and Chemokines) and Therapy","volume":"34","author":"Antonelli","year":"2020","journal-title":"Best Pract Res Clin Endocrinol Metab"},{"key":"B7","doi-asserted-by":"publisher","DOI":"10.21037\/apm-21-2248","article-title":"A Systematic Review and Meta-Analysis of the Clinical Efficacy and Safety of Chinese Patent Medicines in Treating Subacute Thyroiditis","volume":"10","author":"Yu","year":"2021","journal-title":"Ann Palliat Med"},{"key":"B8","doi-asserted-by":"publisher","DOI":"10.1089\/acm.2017.0086","article-title":"Effect of Goiter Dispersion Formula on Serum Cytokines in Hyperthyroidism Patients With Neurologic Manifestations of Graves\u2019 Disease: A Randomized Trial on 80 Cases","volume":"24","author":"Tian","year":"2018","journal-title":"J Altern Complement Med"},{"key":"B9","doi-asserted-by":"publisher","DOI":"10.1080\/13880209.2016.1260595","article-title":"Clinical Efficacy of Yingliu Mixture Combined With Metimazole for Treating Diffuse Goitre With Hyperthyroidism and Its Impact on Related Cytokines","volume":"55","author":"Yang","year":"2017","journal-title":"Pharm Biol"},{"key":"B10","doi-asserted-by":"publisher","first-page":"535","DOI":"10.1186\/s12906-017-2036-1","article-title":"MOK, a Pharmacopuncture Medicine, Regulates Thyroid Dysfunction in L-Thyroxin-Induced Hyperthyroidism in Rats Through the Regulation of Oxidation and the TRPV1 Ion Channel","volume":"17","author":"Hwang","year":"2017","journal-title":"BMC Complement Altern Med"},{"key":"B11","doi-asserted-by":"publisher","DOI":"10.1016\/j.explore.2020.10.007","article-title":"Successful Intervention With Chinese Herbal Medicine for Hyperthyroidism: Two Case Reports and a Literature Review","volume":"17","author":"Lin","year":"2021","journal-title":"Explore (NY)"},{"key":"B12","doi-asserted-by":"publisher","DOI":"10.1097\/JCMA.0000000000000547","article-title":"Goiter Disease in Traditional Chinese Medicine: Modern Insight Into Ancient Wisdom","volume":"84","author":"Lee","year":"2021","journal-title":"J Chin Med Assoc"},{"key":"B13","first-page":"106421633B","article-title":"The Components and Preparations of a New Prescription for Hashimoto\u2019s Thyroiditis","author":"Liu","year":"2019","journal-title":"China Patent CN"},{"key":"B14","article-title":"Protective Effects of Jiayan Kangtai Granules on Autoimmune Thyroiditis in a Rat Model by Modulating Th17\/Treg Cell Balance","volume":"38","author":"Hou","year":"2018","journal-title":"J Tradit Chin Med"},{"key":"B15","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2020.110288","article-title":"Prunella Vulgaris L. Attenuates Experimental Autoimmune Thyroiditis by Inducing Indoleamine 2,3-Dioxygenase 1 Expression and Regulatory T Cell Expansion","volume":"128","author":"Qiu","year":"2020","journal-title":"BioMed Pharmacother"},{"key":"B16","first-page":"195","article-title":"Total Area of Inflammatory Infiltrate and Percentage of Inflammatory Infiltrate Identify Different Clinical-Serological Subsets of Primary Sj\u00f6gren\u2019s Syndrome Better Than Traditional Histopathological Parameters","volume":"126","author":"Donati","year":"2020","journal-title":"Clin Exp Rheumatol"},{"key":"B17","doi-asserted-by":"publisher","DOI":"10.3389\/fcell.2021.685522","article-title":"Metformin Reverses Hashimoto\u2019s Thyroiditis by Regulating Key Immune Events","volume":"9","author":"Jia","year":"2021","journal-title":"Front Cell Dev Biol"},{"key":"B18","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2011.03.017","article-title":"Effects of Modified Haizao Yuhu Decoction in Experimental Autoimmune Thyroiditis Rats","volume":"135","author":"Song","year":"2011","journal-title":"J Ethnopharmacol"},{"key":"B19","article-title":"Edaravone Ameliorates Experimental Autoimmune Thyroiditis in Rats Through HO-1-Dependent STAT3\/PI3K\/Akt Pathway","volume":"10","author":"Li","year":"2018","journal-title":"Am J Transl Res"},{"key":"B20","doi-asserted-by":"publisher","DOI":"10.1016\/j.semcancer.2019.05.019","article-title":"Thyroid Autoimmune Disorders and Cancer","volume":"64","author":"Ferrari","year":"2020","journal-title":"Semin Cancer Biol"},{"key":"B21","doi-asserted-by":"publisher","DOI":"10.1016\/j.autrev.2020.102649","article-title":"Hashimoto\u2019s Thyroiditis: An Update on Pathogenic Mechanisms, Diagnostic Protocols, Therapeutic Strategies, and Potential Malignant Transformation","volume":"19","author":"Ralli","year":"2020","journal-title":"Autoimmun Rev"},{"key":"B22","doi-asserted-by":"publisher","first-page":"55","DOI":"10.1007\/s12026-016-8819-5","article-title":"Adjuvants- and Vaccines-Induced Autoimmunity: Animal Models","volume":"65","author":"Ruiz","year":"2017","journal-title":"Immunol Res"},{"key":"B23","doi-asserted-by":"publisher","first-page":"299","DOI":"10.1186\/s12974-018-1341-z","article-title":"Hashimoto\u2019s Thyroiditis Induces Neuroinflammation and Emotional Alterations in Euthyroid Mice","volume":"15","author":"Cai","year":"2018","journal-title":"J\u00a0Neuroinflammation"},{"key":"B24","doi-asserted-by":"publisher","DOI":"10.1089\/thy.2019.0711","article-title":"A Novel Mouse Model of Autoimmune Thyroiditis Induced by Immunization With Adenovirus Containing Full-Length Thyroglobulin cDNA: Implications to Genetic Studies of Thyroid Autoimmunity","volume":"30","author":"Faustino","year":"2020","journal-title":"Thyroid"},{"key":"B25","doi-asserted-by":"publisher","DOI":"10.3109\/08830180009088510","article-title":"Newer Insights Into the Pathogenesis of Experimental Autoimmune Thyroiditis","volume":"19","author":"Stafford","year":"2000","journal-title":"Int Rev Immunol"},{"key":"B26","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.27180","article-title":"The Pathogenesis of Thyroid Autoimmune Diseases: New T Lymphocytes-Cytokines Circuits Beyond the Th1-Th2 Paradigm","volume":"234","author":"Li","year":"2019","journal-title":"J Cell Physiol"},{"key":"B27","doi-asserted-by":"publisher","DOI":"10.1089\/thy.2019.0552","article-title":"Elevated microRNA-326 Levels Regulate the IL-23\/IL-23r\/Th17 Cell Axis in Hashimoto\u2019s Thyroiditis by Targeting a Disintegrin and Metalloprotease 17","volume":"30","author":"Liu","year":"2020","journal-title":"Thyroid"},{"key":"B28","doi-asserted-by":"publisher","DOI":"10.4183\/aeb.2019.74","article-title":"Serum Levels of Interleukin-23 and Interleukin-17 in Hashimoto\u2019s Thyroiditis","volume":"5","author":"Gerenova","year":"2019","journal-title":"Acta Endocrinol (Buchar)"},{"key":"B29","doi-asserted-by":"publisher","first-page":"e11211","DOI":"10.1097\/MD.0000000000011211","article-title":"Heterogeneity of Tissue IL-17 and Tight Junction Proteins Expression Demonstrated in Patients With Autoimmune Thyroid Diseases","volume":"97","author":"Zake","year":"2018","journal-title":"Med (Baltimore)"},{"key":"B30","doi-asserted-by":"publisher","DOI":"10.1007\/s12020-021-02688-z","article-title":"Vitamin D Deficiency is Associated With Thyroid Autoimmunity: Results From an Epidemiological Survey in Tianjin, China","volume":"73","author":"Fang","year":"2021","journal-title":"Endocrine"},{"key":"B31","doi-asserted-by":"publisher","first-page":"690","DOI":"10.19852\/j.cnki.jtcm.2020.04.019","article-title":"Clinical Research Linking Traditional Chinese Medicine Constitution Types With Diseases: A Literature Review of 1639 Observational Studies","volume":"40","author":"Liang","year":"2020","journal-title":"J Tradit Chin Med"}],"container-title":["Frontiers in Endocrinology"],"original-title":[],"link":[{"URL":"https:\/\/www.frontiersin.org\/articles\/10.3389\/fendo.2021.794568\/full","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,1,31]],"date-time":"2022-01-31T14:53:21Z","timestamp":1643640801000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.frontiersin.org\/articles\/10.3389\/fendo.2021.794568\/full"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,1,31]]},"references-count":31,"alternative-id":["10.3389\/fendo.2021.794568"],"URL":"https:\/\/doi.org\/10.3389\/fendo.2021.794568","relation":{},"ISSN":["1664-2392"],"issn-type":[{"value":"1664-2392","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,1,31]]},"article-number":"794568"}}