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Endocrinol."],"abstract":"<jats:sec><jats:title>Introduction<\/jats:title><jats:p>Trace metal elements may play a crucial role in bone mineralization and metabolism. However, the quantification of trace element concentrations in human bone tissue has received little attention.<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials and methods<\/jats:title><jats:p>Bone tissue samples were collected from 55 elderly patients (15 males and 40 females) with intertrochanteric hip fractures. The calcium, phosphorus, manganese, iron, copper, and zinc concentrations in the cortical bone zone, cancellous bone zone, and junction zone between cortical and cancellous bone were determined by energy-dispersive X-ray fluorescence (EDX). The differences in trace element concentrations in the three regions were compared, and the correlation between gender and bone trace element contents of the bones was analyzed using the Kruskal-Wallis\u2019s test. The correlation between age, body mass index (BMI), and bone calcium, phosphorus concentrations, and trace elements in three bone zones was determined using Spearman correlation analysis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The Kruskal-Wallis test showed no difference in bone phosphorus concentration among the three regions. In contrast, the difference in the concentrations of bone calcium and four metal elements was statistically significant (<jats:italic>P<\/jats:italic>&amp;lt;0.01). In addition, no statistical differences were observed in the concentrations of trace elements among the three regions in elderly male and female patients. Spearman correlation analysis showed a strong negative correlation between bone calcium and phosphorus in three bone regions (r=-0.999, -0.95, -0.998, <jats:italic>P<\/jats:italic> &amp;lt; 0.01) and a significant positive correlation between trace metal elements in the cancellous bone zone. In the junction zone, the BMI showed a strong positive correlation with bone calcium content (r=0.347, <jats:italic>P<\/jats:italic>=0.009) and a significant negative correlation with phosphorus content (r=-0.349, <jats:italic>P<\/jats:italic>=0.009).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Bone calcium and phosphorus were the main components of hydroxyapatite, and these two elements accounted for the majority of bone mineral salts. Trace metal elements are essential for bone metabolism and specific synergistic interactions. BMI may be associated with bone calcium and phosphorus contents in elderly patients with osteoporosis.<\/jats:p><\/jats:sec>","DOI":"10.3389\/fendo.2022.1005637","type":"journal-article","created":{"date-parts":[[2022,12,13]],"date-time":"2022-12-13T04:55:17Z","timestamp":1670907317000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":9,"title":["The concentrations of bone calcium, phosphorus and trace metal elements in elderly patients with intertrochanteric hip fractures"],"prefix":"10.3389","volume":"13","author":[{"given":"Shangjin","family":"Lin","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cong","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoxi","family":"Cai","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fengjian","family":"Yang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"YongQian","family":"Fan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2022,12,13]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"53","DOI":"10.5468\/ogs.2017.60.1.53","article-title":"Effects of vitamin d deficiency and daily calcium intake on bone mineral density and osteoporosis in Korean postmenopausal woman","volume":"60","author":"Chon","year":"2017","journal-title":"ObstetGynecol Sci"},{"key":"B2","doi-asserted-by":"publisher","first-page":"86","DOI":"10.1016\/j.jtemb.2015.06.005","article-title":"Effects of thirty elements on bone metabolism","volume":"32","author":"Dermience","year":"2015","journal-title":"J Trace Elem Med Biol"},{"key":"B3","doi-asserted-by":"publisher","first-page":"39","DOI":"10.1016\/j.jtemb.2013.07.004","article-title":"The concentration of manganese, iron, and strontium in hip joint bone obtained from patients undergoing hip replacement surgery","volume":"28","author":"Budis","year":"2014","journal-title":"J Trace Elem Med Biol"},{"key":"B4","doi-asserted-by":"publisher","DOI":"10.1007\/s10534-016-9952-2","article-title":"Iron uptake and transport across physiological barriers","volume":"29","author":"Duck","year":"2016","journal-title":"Biometals"},{"key":"B5","doi-asserted-by":"publisher","first-page":"695","DOI":"10.1007\/s00394-012-0375-8","article-title":"Relationship between vitamin d deficiency, bone remodelling and iron status in iron-deficient young men consuming an iron-fortified food","volume":"52","author":"Blanco-Rojo","year":"2013","journal-title":"Eur J Nutr"},{"key":"B6","doi-asserted-by":"publisher","first-page":"7","DOI":"10.3892\/etm.2015.2484","article-title":"Reducing iron accumulation: A potential approach for the prevention and treatment of postmenopausal osteoporosis","volume":"10","author":"Chen","year":"2015","journal-title":"Exp Ther Med"},{"key":"B7","doi-asserted-by":"publisher","DOI":"10.1007\/s12011-021-02845-5","article-title":"Associations of copper intake with bone mineral density and osteoporosis in adults: Data from the national health and nutrition examination survey","volume":"200","author":"Fan","year":"2022","journal-title":"Biol Trace Elem Res"},{"key":"B8","doi-asserted-by":"publisher","DOI":"10.1002\/jbm.a.35032","article-title":"Effects of Cu-doped 45S5 bioactive glass on the lipid peroxidation-associated growth of human osteoblast-like cells in vitro","volume":"102","author":"Milkovic","year":"2014","journal-title":"J BioMed Mater Res A"},{"key":"B9","doi-asserted-by":"publisher","DOI":"10.1002\/biof.5520300405","article-title":"Role of endogenous zinc in bones of newborn rats","volume":"30","author":"Drzazga","year":"2007","journal-title":"Biofactors"},{"key":"B10","doi-asserted-by":"publisher","first-page":"77","DOI":"10.1016\/j.jsbmb.2016.04.006","article-title":"Vitamin D3 transactivates the zinc and manganese transporter SLC30A10 via the vitamin d receptor","volume":"163","author":"Claro da Silva","year":"2016","journal-title":"J Steroid BiochemMolBiol"},{"key":"B11","doi-asserted-by":"publisher","DOI":"10.1177\/003335491513000408","article-title":"U.S. department of health and human services federal panel on community water fluoridation. 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