{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T15:52:51Z","timestamp":1753890771434,"version":"3.41.2"},"reference-count":44,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2023,1,12]],"date-time":"2023-01-12T00:00:00Z","timestamp":1673481600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100017700","name":"Henan Provincial Science and Technology Research Project","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100017700","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Endocrinol."],"abstract":"<jats:sec><jats:title>Objective<\/jats:title><jats:p>This study aimed to determine whether controlled ovarian hyperstimulation (COH) parameters influence the incidence of <jats:italic>de novo<\/jats:italic> chromosomal abnormalities (&amp;gt; 4 Mb) in blastocysts and, thus, clinical pregnancy outcomes in preimplantation genetic testing (PGT).<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Couples who underwent preimplantation genetic testing for structural chromosome rearrangements (PGT-SR) and monogenic disorders (PGT-M) were included in this study. The relationships of maternal age, paternal age, stimulation protocol, exogenous gonadotropin dosage, duration of stimulation, number of oocytes retrieved and estradiol (E<jats:sub>2<\/jats:sub>) levels on human chorionic gonadotropin (hCG) trigger day with the incidence of <jats:italic>de novo<\/jats:italic> chromosomal abnormalities were assessed. Blastocysts were biopsied, and nuclear DNA was sequenced using next-generation sequencing (NGS). Clinical pregnancy outcomes after single euploid blastocyst transfers under different COH parameters were assessed. <\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>A total of 1,710 and 190 blastocysts were biopsied for PGT-SR and PGT-M, respectively. The rate of <jats:italic>de novo<\/jats:italic> chromosomal abnormalities was found to increase with maternal age (<jats:italic>p&amp;lt;<\/jats:italic> 0.001) and paternal age (<jats:italic>p<\/jats:italic> = 0.019) in the PGT-SR group. No significant differences in the incidence of <jats:italic>de novo<\/jats:italic> chromosomal abnormalities were seen for different maternal or paternal age groups between the PGT-SR and PGT-M groups (<jats:italic>p<\/jats:italic> &amp;gt; 0.05). Stratification analysis by gonadotropin dosage, stimulation protocol, duration of stimulation, number of retrieved oocytes and E<jats:sub>2<\/jats:sub> levels on hCG trigger day revealed that <jats:italic>de novo<\/jats:italic> chromosomal abnormalities and clinical pregnancy outcomes were not correlated with COH parameters after adjusting for various confounding factors.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>The rate of <jats:italic>de novo<\/jats:italic> chromosomal abnormalities was found to increase with maternal or paternal age. COH parameters were found to not influence the incidence of <jats:italic>de novo<\/jats:italic> chromosomal abnormalities or clinical pregnancy outcomes.<\/jats:p><\/jats:sec>","DOI":"10.3389\/fendo.2022.1080843","type":"journal-article","created":{"date-parts":[[2023,1,12]],"date-time":"2023-01-12T04:52:35Z","timestamp":1673499155000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":1,"title":["Controlled ovarian hyperstimulation parameters are not associated with de novo chromosomal abnormality rates and clinical pregnancy outcomes in preimplantation genetic 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