{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,19]],"date-time":"2025-11-19T09:39:20Z","timestamp":1763545160735,"version":"3.41.2"},"reference-count":53,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2022,12,5]],"date-time":"2022-12-05T00:00:00Z","timestamp":1670198400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Endocrinol."],"abstract":"<jats:p>Polo-like kinase 1 (<jats:italic>Plk1<\/jats:italic>) has multiple functions in the cell cycle, including in the maturation of centrosomes during the G2\/M transition, the separation of centrosomes, and the activation of cyclin-dependent kinase 1 expression and spindle assembly. In this study, we investigated the potential regulatory roles of <jats:italic>Plk1<\/jats:italic> in the reproductive development of the male oriental river prawn (<jats:italic>Machrobrachium nipponense<\/jats:italic>). The full cDNA sequence of <jats:italic>Mn<\/jats:italic>-<jats:italic>Plk1<\/jats:italic> was 2360 base pairs long, with an open reading frame of 1836 base pairs encoding 611 amino acids. Protein sequence alignment identified a conserved serine\/threonine kinase domain and two Polo-boxes. Phylogenetic tree analysis revealed that <jats:italic>Mn<\/jats:italic>-<jats:italic>Plk1<\/jats:italic> had the closest evolutionary distance with <jats:italic>Plk1<\/jats:italic>s of freshwater prawns and then with those of crustacean species, whereas the evolutionary distance with mollusks was much more distant. Quantitative PCR analysis predicted that <jats:italic>Mn<\/jats:italic>-<jats:italic>Plk1<\/jats:italic> plays essential roles in the regulation of gonad development. RNA interference analysis and histological observations showed that expression of insulin-like androgenic gland hormone decreased as the expression of <jats:italic>Mn-Plk1<\/jats:italic> decreased, and fewer than 5% of cells were sperm cells at day 14 in the <jats:italic>dsPlk1<\/jats:italic> injected prawns. This result indicated that <jats:italic>Plk1<\/jats:italic> positively regulated testis development in <jats:italic>M. nipponense<\/jats:italic> by affecting the expression of this hormone. Our results highlight the functions of <jats:italic>Plk1<\/jats:italic> in <jats:italic>M<\/jats:italic>. <jats:italic>nipponense<\/jats:italic> and provide valuable information that can be applied to establish artificial techniques to regulate testis development in this species.<\/jats:p>","DOI":"10.3389\/fendo.2022.1084802","type":"journal-article","created":{"date-parts":[[2022,12,5]],"date-time":"2022-12-05T04:44:18Z","timestamp":1670215458000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":4,"title":["Identification of potential functions of polo-like kinase 1 in male reproductive development of the oriental river prawn (Macrobrachium nipponense) by RNA interference 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